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The map label for this gene is lip2 [H]

Identifier: 94312423

GI number: 94312423

Start: 3782733

End: 3783689

Strand: Reverse

Name: lip2 [H]

Synonym: Rmet_3492

Alternate gene names: 94312423

Gene position: 3783689-3782733 (Counterclockwise)

Preceding gene: 94312424

Following gene: 94312422

Centisome position: 96.32

GC content: 65.94

Gene sequence:

>957_bases
ATGGCCATCGACCCCCAGCTACTCAGCTACTACCGCCAGATGGCGGACCTGTACGCCAACGAACCCATTCCCGAGGATGC
CGCAGCGCGGCGCTCGCGCTTCGAGGATGTGGCCGCGCGCTCGCGACAGCCGGACACCGAGGAGATCTCCGTCACCGATC
TCGCGATCCCCGTGGACGGCGCGGCGTTGACGGTGCGCTTGTTCAGGCCCGCCAATGTTGAGCAGCCTCGACTGATTGTC
TATTTCCACGGTGGTGGCTGGGTCGTCGGCTCGCCGGAAACGCATCACACGGTGGCGGCGCTGCTGGCAGCCGACACACA
GTGCGTCGTTGCCAGTGTCGACTACAGGCTGGCCCCGGAATACGCGTTTCCAACCCCTTGCGACGATGCGGTGGCAGCCG
TGCAATGGCTCGCTGCATCGCGCGAGTCGCTTGGCGTGCATCCAGATTTTCTCGCCGTGGCTGGCGACAGCGCAGGCGCG
CATCTGGCTGCGGCAGCGGCCCGCGTCGTCAACGAACAGCGCCGTGCGCATATCGTCCAGGCGCAGTTGCTGATCTACCC
CGTGGCTGCGCCATTGCTCACGACCGAGAGCTATCGGGCGTTCGCGAACGGGCCTGGTCTCACCCGAGACGAAATGGCGT
GGTACTGGACGCAGTTCATCGGCGCCGAAGAACTGGCCAAGGGCGCCGAGCAGGACGATCCCCGGATCCATCTGATGGCA
TCGCCGCCCGCCATGTTGCCCCCCGCCAGCGTCGTGCTTGTGGCGGCCAATGACGTGCTGCGTGATGACGGCCTGGCCTA
CGCCAACTTCCTCGTGCGCCACGATGCACCGGTGATCACGATCGAGGCGTCAGGTATGACACATGGCTTTGCGCGTCTGC
AACCGGACGCACCACGCGCACGGGAGTGGATGCGCCGCGCCGCCGAGGCGTTCACAGGTGTGTTCGACGACCTCTGA

Upstream 100 bases:

>100_bases
ATTCGGGGGATAAGAAAAATCGGGTTTCTGATTTTGTGTTCGGCAGCGCATTCAGCGTTACGATTCGAAACGGTTAGCAA
AGCGATTTTCGAAGTTTCCG

Downstream 100 bases:

>100_bases
GGACCGCCGAGGACCGCTGAGGCCCGATCCAGCGCAAGCATTCGTGTGGATATCACTCGCCATAGGGCTTGCGGGTGCGG
GCATCCCTTAGAATGTCGGT

Product: esterase/lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDGAALTVRLFRPANVEQPRLIV
YFHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPEYAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGA
HLAAAAARVVNEQRRAHIVQAQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMA
SPPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRAREWMRRAAEAFTGVFDDL

Sequences:

>Translated_318_residues
MAIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDGAALTVRLFRPANVEQPRLIV
YFHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPEYAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGA
HLAAAAARVVNEQRRAHIVQAQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMA
SPPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRAREWMRRAAEAFTGVFDDL
>Mature_317_residues
AIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDGAALTVRLFRPANVEQPRLIVY
FHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPEYAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGAH
LAAAAARVVNEQRRAHIVQAQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMAS
PPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRAREWMRRAAEAFTGVFDDL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=294, Percent_Identity=26.1904761904762, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI157041239, Length=158, Percent_Identity=34.1772151898734, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI206597554, Length=180, Percent_Identity=36.6666666666667, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI68051721, Length=182, Percent_Identity=32.4175824175824, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI61966717, Length=169, Percent_Identity=32.5443786982249, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI226423947, Length=190, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157041237, Length=143, Percent_Identity=31.4685314685315, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786682, Length=268, Percent_Identity=28.7313432835821, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17567059, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=141, Percent_Identity=34.0425531914894, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=167, Percent_Identity=30.5389221556886, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=310, Percent_Identity=23.8709677419355, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=85, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=85, Percent_Identity=35.2941176470588, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=37.7551020408163, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=37.7551020408163, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=37.7551020408163, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34535; Mature: 34404

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDG
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
AALTVRLFRPANVEQPRLIVYFHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPE
CEEEEEEECCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCEEEEEECCCEECCC
YAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGAHLAAAAARVVNEQRRAHIVQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMA
HHHHEEEHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEE
SPPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRA
CCCCCCCCCCEEEEECCHHHHCCCHHHHHHHEECCCCEEEEEECCCCCCHHCCCCCCHHH
REWMRRAAEAFTGVFDDL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDG
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
AALTVRLFRPANVEQPRLIVYFHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPE
CEEEEEEECCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCEEEEEECCCEECCC
YAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGAHLAAAAARVVNEQRRAHIVQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMA
HHHHEEEHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEE
SPPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRA
CCCCCCCCCCEEEEECCHHHHCCCHHHHHHHEECCCCEEEEEECCCCCCHHCCCCCCHHH
REWMRRAAEAFTGVFDDL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]