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The map label for this gene is rep [H]

Identifier: 94312409

GI number: 94312409

Start: 3758541

End: 3760628

Strand: Reverse

Name: rep [H]

Synonym: Rmet_3478

Alternate gene names: 94312409

Gene position: 3760628-3758541 (Counterclockwise)

Preceding gene: 94312416

Following gene: 94312408

Centisome position: 95.74

GC content: 63.75

Gene sequence:

>2088_bases
ATGTCCCACGGGCTCAATTCCGCGCAATCCGAAGCCGTTCGCTATTTCGACGGGCCATGCCTCGTCCTGGCGGGTGCCGG
ATCGGGCAAGACGCGCGTGATCACGCAGAAGATCGCGCACCTGATCGAGGACAAGGGGTTCGAGCCGCGCCACATCGCCG
CCGTCACGTTCACCAACAAGGCGGCCAAGGAGATGCAGGAGCGCATCGGCAAGCTGATGGAGGGCAAGACCACGCGCGAG
GGCAAGCGGATTCCGCTCAAGCAGCTCACGGTCTGCACGTTCCATTCGCTCGGCGTGCAGATCCTGCGCATGGAAGCCGA
GCACCTTGGCCTGAAGCCGCAGTTCTCGATCATGGATTCCGATGACTGCTTCGGCCTGGTGCAGGAGCAGCTTGGCACCA
CGGACAAGAAGCTGATCCGCAGCGTGCAGGGCACGATCTCGCTGTGGAAGAACGGCATGATCGATCCGGAGACGGCGCTC
GCGCAGGCGAGCGATGCCGACGAGCACCAGGCCGCGATGGTGTATCGCAATTACGTGGCCACGCTGCACGCGTACCAGGC
GGTGGACTTCGACGACCTGATCCGCCTGCCCGCCGAATTGTTCGCGCGCGACGAAGCCGTGCAATTGAAGTGGCAGAACC
GCCTGCGCTACTTCCTCGTCGACGAATACCAGGACACCAACGCGTGCCAGTACCAGTTGCTCAAGCTGCTGGCCGGTGGC
TCGCATCTGCGCGCGCCGGCGTTCACCGCAGTGGGTGATGACGATCAGGCGATCTATGGTTGGCGTGGCGCCACGCTCGA
CAACCTGCGCCTGCTGCAGACCGATTTTCCGAGTCTGAAGGTGGTCAAGCTCGAACAGAACTACCGTTCGACCGTGCGGA
TTCTGGAAGCCGCCAACGCGGTGATCGCCAACAACCCGAAGCTGTTCGACAAGAAGCTGTGGTCGGAACACGGCATGGGC
GATCCGATCGCGATCCACCCGATGAACGACGAGGAGCACGAGGCAGAGTCAGTGGTATTCCGGCTGTCCGCGCACAAGTT
CGAGCGGCGCGCGCAGTTCCGTGACTACGCAATCCTTTATCGCGGCAATCACCAGGCCCGGCTGTTCGAACAGATCCTGC
GCCGCGAGCGCATTCCGTATGTGCTCTCGGGCGGTCAGAGCTTCTTCGACAAGGCCGAGATCAAGGACATCTGCGCGTAC
CTGCGCCTGATCGCCAACCCCGACGACGATCCCGCATTCATCCGCGCGATCACCACGCCGCGTCGTGGCGTGGGCAACAC
CACGCTCGAAGTGCTCGGCACGTTCGCGGGCCAGGCCAAGGTATCGCTGTTCGAGGCGGCCATGATGGGTGGCATCGAGG
CGAAGCTGCAGCCGCGCCAGCTTGAGCCGCTGCGCACGTTCTGCGAGTCGATGGTACGGCTGGCCGATCGTGCGAAGACA
GACCCGGCCACGGTGGTGCTCGACGATCTCATGGAAGGCATCCACTACGAGGCCTACCTCTACGACACCTTCGACGAACG
GCAAGCGCAATCGCGCTGGACCAACACGCTCGAATTCCTCGACTGGCTCAAACGCAAGGGCACGCGTCCCGAGCCGGAAG
GCGAGGGCGAAGCCACCGGATTCGACACCGCCGATGGTTTCGGCGACGAAGGCAAGAACCTGCTCGAGCTTACGCAGACC
GTGGCGCTGATGAGCATGCTGGAAGGCCGCGAGGAAGATCCCGATGCGGTGCGACTGTCGACGCTGCACGCGTCGAAAGG
GCTCGAGTATCCGCACGTGTTCCTGGTCGGCGTGGAGGAGGGCATCCTGCCTCACTCGCGCGAGGACGAGGAAATGGGCG
ACGAGAAGATCGAGGAGGAGCGCCGCCTGATGTATGTGGGCATCACGCGCGCGCAGCGCAGTCTGCAGATCAGCTGGTGC
AGGAAGCGCAAGCGCGCCCGCGATACGTATGCGTGCGAGCCGTCGCGTTTCATCGCCGAGATGAAGCTCGAGGACAGTCC
CGCCCCGAAGGACGAGACTCCGGCCATGAGCCCGAAGGATCGTCTGGCCGGGCTCAAGGCGCTGCTCGGCAAGCCTGCGG
CGGAATGA

Upstream 100 bases:

>100_bases
ACGAGGCGCGTGTTAACATCGCCAGCTTTGGCGCACCGGCTCCGGTGTGCTTCATCCGCGCCTTTTCGGCCGATCCAGGC
CAATCCTCATCAAAGATCAC

Downstream 100 bases:

>100_bases
TTGATACAAGCCGGACAAGCTTCGCGATGACCGGCTCTCTACGATTGGAATGCGCGCTTTCGTCTCGACGTCTGTCGCGA
CACACTGCGCCTACCCTTCG

Product: DNA helicase and single-stranded DNA-dependent ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSHGLNSAQSEAVRYFDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTRE
GKRIPLKQLTVCTFHSLGVQILRMEAEHLGLKPQFSIMDSDDCFGLVQEQLGTTDKKLIRSVQGTISLWKNGMIDPETAL
AQASDADEHQAAMVYRNYVATLHAYQAVDFDDLIRLPAELFARDEAVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPSLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMG
DPIAIHPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANPDDDPAFIRAITTPRRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRTFCESMVRLADRAKT
DPATVVLDDLMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTRPEPEGEGEATGFDTADGFGDEGKNLLELTQT
VALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHSREDEEMGDEKIEEERRLMYVGITRAQRSLQISWC
RKRKRARDTYACEPSRFIAEMKLEDSPAPKDETPAMSPKDRLAGLKALLGKPAAE

Sequences:

>Translated_695_residues
MSHGLNSAQSEAVRYFDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTRE
GKRIPLKQLTVCTFHSLGVQILRMEAEHLGLKPQFSIMDSDDCFGLVQEQLGTTDKKLIRSVQGTISLWKNGMIDPETAL
AQASDADEHQAAMVYRNYVATLHAYQAVDFDDLIRLPAELFARDEAVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPSLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMG
DPIAIHPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANPDDDPAFIRAITTPRRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRTFCESMVRLADRAKT
DPATVVLDDLMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTRPEPEGEGEATGFDTADGFGDEGKNLLELTQT
VALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHSREDEEMGDEKIEEERRLMYVGITRAQRSLQISWC
RKRKRARDTYACEPSRFIAEMKLEDSPAPKDETPAMSPKDRLAGLKALLGKPAAE
>Mature_694_residues
SHGLNSAQSEAVRYFDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTREG
KRIPLKQLTVCTFHSLGVQILRMEAEHLGLKPQFSIMDSDDCFGLVQEQLGTTDKKLIRSVQGTISLWKNGMIDPETALA
QASDADEHQAAMVYRNYVATLHAYQAVDFDDLIRLPAELFARDEAVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGGS
HLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPSLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMGD
PIAIHPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYL
RLIANPDDDPAFIRAITTPRRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRTFCESMVRLADRAKTD
PATVVLDDLMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTRPEPEGEGEATGFDTADGFGDEGKNLLELTQTV
ALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHSREDEEMGDEKIEEERRLMYVGITRAQRSLQISWCR
KRKRARDTYACEPSRFIAEMKLEDSPAPKDETPAMSPKDRLAGLKALLGKPAAE

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=699, Percent_Identity=46.20886981402, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI2367296, Length=664, Percent_Identity=36.144578313253, Blast_Score=354, Evalue=9e-99,
Organism=Saccharomyces cerevisiae, GI6322369, Length=738, Percent_Identity=27.3712737127371, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=654, Percent_Identity=23.7003058103976, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78375; Mature: 78244

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHGLNSAQSEAVRYFDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNK
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCH
AAKEMQERIGKLMEGKTTREGKRIPLKQLTVCTFHSLGVQILRMEAEHLGLKPQFSIMDS
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
DDCFGLVQEQLGTTDKKLIRSVQGTISLWKNGMIDPETALAQASDADEHQAAMVYRNYVA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
TLHAYQAVDFDDLIRLPAELFARDEAVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
HHHHHHCCCHHHHHHCCHHHHCCCHHEEEEHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPSLKVVKLEQNYRSTVRILEAANA
CCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHCH
VIANNPKLFDKKLWSEHGMGDPIAIHPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANPDDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRTFCESMVRLADRAKT
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DPATVVLDDLMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTRPEPEGEGEATG
CCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FDTADGFGDEGKNLLELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEEEEEEECC
GILPHSREDEEMGDEKIEEERRLMYVGITRAQRSLQISWCRKRKRARDTYACEPSRFIAE
CCCCCCCCCHHHCHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MKLEDSPAPKDETPAMSPKDRLAGLKALLGKPAAE
EECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHGLNSAQSEAVRYFDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNK
CCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCH
AAKEMQERIGKLMEGKTTREGKRIPLKQLTVCTFHSLGVQILRMEAEHLGLKPQFSIMDS
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
DDCFGLVQEQLGTTDKKLIRSVQGTISLWKNGMIDPETALAQASDADEHQAAMVYRNYVA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
TLHAYQAVDFDDLIRLPAELFARDEAVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
HHHHHHCCCHHHHHHCCHHHHCCCHHEEEEHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPSLKVVKLEQNYRSTVRILEAANA
CCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHCH
VIANNPKLFDKKLWSEHGMGDPIAIHPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANPDDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRTFCESMVRLADRAKT
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DPATVVLDDLMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTRPEPEGEGEATG
CCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FDTADGFGDEGKNLLELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEEEEEEECC
GILPHSREDEEMGDEKIEEERRLMYVGITRAQRSLQISWCRKRKRARDTYACEPSRFIAE
CCCCCCCCCHHHCHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MKLEDSPAPKDETPAMSPKDRLAGLKALLGKPAAE
EECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]