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The map label for this gene is menB [C]

Identifier: 94312401

GI number: 94312401

Start: 3749855

End: 3750655

Strand: Reverse

Name: menB [C]

Synonym: Rmet_3470

Alternate gene names: 94312401

Gene position: 3750655-3749855 (Counterclockwise)

Preceding gene: 94312405

Following gene: 94312400

Centisome position: 95.48

GC content: 65.79

Gene sequence:

>801_bases
GTGGAAATGGAATCCGGCGGCAAGGCTGTTGACCCCTTCGTGTTACGAGAGACCCATGATGGCGTGGTGACGCTACGGCT
GAACCGGCCGCAGCAGTTCAACGCGCTGTCCGAAGCCATGCTCGAAGCGCTGCGCGTGGCCTTCGAGGATGTCGCCCGGG
ACGACTCGGTACGTTGCGTCGTGCTGGCCGCCGAAGGCAAGGCGTTCTGCGCCGGGCACGATCTGCGCGAGATGCGCGGT
CGCCCGGACCTGGCTTACTACCGCACGCTGTTCGCGCAATGCAGCGTGGTCATGCAGGCGATCCAGGCATTGCCGGTGCC
GGTCATCGCGCGGGTGCACGGCATTGCCACGGCCGCCGGCTGCCAGCTCGTGGCGAGTTGCGACCTGGCTATTGCGTCGG
TTGCGGCGAAGTTCGCGGTGTCCGGCATCAACGTCGGCTTGTTCTGCTCCACGCCATCGGTGGCGTTGTCGCGCAATGTC
TCGACCAAACGCGCGTTCGACCTGCTCGTAACCGGCCGCTTCATCGATGCCGAGACGGCAGCGGATTGGGGCCTGATCAA
CGAGGCCGTGCCCGAGCAGGAGCTTGACGCCGCCGTGGCGCGCAAGGCGGAGGCGATTCTGTCCAAGAGCCCGGCAGCGA
TTCGCTACGGCAAGGCGATGTTCTATCGCCAGCGCCTGATGGAGCTGGGCGATGCCTATGCATTCGCGGGCGACGTCATG
GCGCGGAACATGATGGAGGAGGATGCGGGCGAGGGCATCGATGCGTTCCTGCAGAAGCGCCCCCCTCGCTGGCAGCGATA
A

Upstream 100 bases:

>100_bases
AATAGGGTCTGGATGGTTCGCCGGCCGGATATCTGCCCGAGTTGACCGGACATTCCTGCAGGTCCGAACCTGGACACTCA
CCCCTTGACGGAGCGCAGAC

Downstream 100 bases:

>100_bases
AGTCTGTCTGCCAGCGTGACCGTGCCGGGCGCGCGGATGTCCCTTTCACGCCGTCCCAACTTTGCAACAGGTCGGGCCAA
CTCGCCTCGGCGTTGCTACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCVVLAAEGKAFCAGHDLREMRG
RPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAGCQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNV
STKRAFDLLVTGRFIDAETAADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVM
ARNMMEEDAGEGIDAFLQKRPPRWQR

Sequences:

>Translated_266_residues
MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCVVLAAEGKAFCAGHDLREMRG
RPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAGCQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNV
STKRAFDLLVTGRFIDAETAADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVM
ARNMMEEDAGEGIDAFLQKRPPRWQR
>Mature_266_residues
MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCVVLAAEGKAFCAGHDLREMRG
RPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAGCQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNV
STKRAFDLLVTGRFIDAETAADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVM
ARNMMEEDAGEGIDAFLQKRPPRWQR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=46.1224489795918, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=28.2868525896414, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=29.1828793774319, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.1828793774319, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=23.5294117647059, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=27.3224043715847, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=31.640625, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=29.7297297297297, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=31.8367346938775, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=29.8449612403101, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788682, Length=236, Percent_Identity=24.1525423728814, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=264, Percent_Identity=39.0151515151515, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=26.530612244898, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=26.9076305220884, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=262, Percent_Identity=26.7175572519084, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=180, Percent_Identity=30.5555555555556, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=25.1824817518248, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=275, Percent_Identity=22.9090909090909, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=27.3584905660377, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=27.906976744186, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=40.1574803149606, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.1338582677165, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=30.188679245283, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=187, Percent_Identity=28.3422459893048, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=190, Percent_Identity=27.3684210526316, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28902; Mature: 28902

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCV
CCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VLAAEGKAFCAGHDLREMRGRPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAG
EEEECCCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNVSTKRAFDLLVTGRFIDAETA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHEECCEECCCCC
ADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVM
CHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNMMEEDAGEGIDAFLQKRPPRWQR
HHHHHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCV
CCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VLAAEGKAFCAGHDLREMRGRPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAG
EEEECCCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNVSTKRAFDLLVTGRFIDAETA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHEECCEECCCCC
ADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVM
CHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNMMEEDAGEGIDAFLQKRPPRWQR
HHHHHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA