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The map label for this gene is hemN [H]

Identifier: 94312405

GI number: 94312405

Start: 3753227

End: 3754729

Strand: Reverse

Name: hemN [H]

Synonym: Rmet_3474

Alternate gene names: 94312405

Gene position: 3754729-3753227 (Counterclockwise)

Preceding gene: 94312407

Following gene: 94312401

Centisome position: 95.59

GC content: 63.74

Gene sequence:

>1503_bases
ATGTCCGCCCCCATGTCCGACACCCCCGATACCGCTATTCCTTCCGGCCTCGCATCCTCGTCTACCGCCTTCGACCGCAC
CGCACTCGCCGACTTCCGCGAACTTGCCGGCCGCATCGATGGCAACGGCCCGCGCTACACGTCGTATCCCACCGCCGATC
GCTTTCACGGTGAAGTCGGCGACGCCGCCTACCGGCGCGCCCTGGATGCCTGCCGTGCGAACGTACCGGCACCGCTGTCG
CTGTACGTCCACATCCCGTTCTGCGAGAACATCTGCTACTACTGCGGCTGCAACAAGATCATCACCAAGGATCACACCCG
CAGCGCGCGTTACGTCGACTATCTGGCACGCGAAATGGCCATGGCCGCCGAGCAGCTTGGCGAGCGCCGCGCGGTCATGC
AGTCGCATTGGGGCGGCGGCACGCCGACGTTCCTCGATCCTGTTGAAATGCGCCACGTGATGTCGGCGTTGCATAAGCAT
TTCGAGCTGCTGCCTGAGGGAGAGCACGCGATCGAGATCGACCCGCGCCGTGTGGGCGACGATCGCATGGAACTGCTCGG
CGAACTTGGCTTCAACCGGATCAGCCTTGGCGTGCAGGACTTCGATCCCGAAGTGCAGCAGGCCATCCACCGGGTGCAGT
CATATGAGGAAACCAACGGCGTGGTGCGGGCCGCGCGACGCCTCGGGTTCCGTTCGATCAGCATGGACCTGATCTACGGA
CTGCCGCACCAGACCACGCAACGCTTCTCGGCAACCATCGACCGCGTGCTGGAAATGCGCCCGGACCGCCTGTCGGTCTA
CAGCTACGCGCACCTCCCGCATGTGTTCAAACCGCAACGCCGCATCGATCCCGCCGCATTGCCACCTGCCGCGGAAAAAC
TCGACATCCTCGTTTCGACGATCGAGCGCCTGACCGCTGCTGGCTATGTCTATATCGGCATGGACCATTTCGCGTTGCCG
AATGACGATCTGGCCATCGCCCAGCGCGAAGGTCGGCTGCAACGCAATTTCCAGGGCTATGCCACCCACGCGGGCTACGA
CCAGCTTGGCTTCGGCGTCTCGGCGATCGGCGCTGTGGCGGGCCACTACGCGCAGAACGCGCGCACCACGGACGAGTACT
ACGCCGCGCTCGACAAAGGCCAACTGCCGATCGTGCGCGGGTTCGAGACCTCGGAAGACGACCGCCTGCGCAAGTTCATC
ATCGGCGAACTGATGTGCAACGGCACGCTCGATATTCCTGCCGTGGAAGCCTCGTACAGCATCAATTTCGCGCAGAAATT
CGCCGCCGAACTGGTCGACCTCGACAAGCTGGCCCTGGACCATCTGCTTCGGCGCACACGGAACCGGATCGAGATCACGC
CGCTGGGTCGACTGCTGGTGCGTCGCATCGCGATGGTGTTCGATCACTATCTGCGTGAAGACGCGCTGCGTCCCAAGCCG
GCAGCGCCGGCGGCCAACGACGCGAAGGTCATCCCGCTGGTGCGCTACTCACGCGTCGTTTAA

Upstream 100 bases:

>100_bases
TGGCGGGCGCACGCATGACGTGGCCTTGATGCTTATCAAGATGGCGTTCCCGGGCGAGGCATAGGATCGGACGTACTTCC
GCCTAGAAAGCACCACAATC

Downstream 100 bases:

>100_bases
AGAAATTTTGATGCTCAGCAAATGAGCCAATAAAAAAAGCCCGCGAGATATTCGCGGGCTTTTTTTGTGGAGCCGGGGTG
ACTTCCGGGGTTGCCCCCGG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVGDAAYRRALDACRANVPAPLS
LYVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMAMAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKH
FELLPEGEHAIEIDPRRVGDDRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYG
LPHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVSTIERLTAAGYVYIGMDHFALP
NDDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVAGHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFI
IGELMCNGTLDIPAVEASYSINFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKP
AAPAANDAKVIPLVRYSRVV

Sequences:

>Translated_500_residues
MSAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVGDAAYRRALDACRANVPAPLS
LYVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMAMAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKH
FELLPEGEHAIEIDPRRVGDDRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYG
LPHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVSTIERLTAAGYVYIGMDHFALP
NDDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVAGHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFI
IGELMCNGTLDIPAVEASYSINFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKP
AAPAANDAKVIPLVRYSRVV
>Mature_499_residues
SAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVGDAAYRRALDACRANVPAPLSL
YVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMAMAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKHF
ELLPEGEHAIEIDPRRVGDDRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYGL
PHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVSTIERLTAAGYVYIGMDHFALPN
DDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVAGHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFII
GELMCNGTLDIPAVEASYSINFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKPA
APAANDAKVIPLVRYSRVV

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=191, Percent_Identity=30.3664921465969, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI87082341, Length=442, Percent_Identity=46.606334841629, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1789325, Length=293, Percent_Identity=29.6928327645051, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 55947; Mature: 55816

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCC
DAAYRRALDACRANVPAPLSLYVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMA
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHH
MAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKHFELLPEGEHAIEIDPRRVGD
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCH
DRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYG
HHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHCC
LPHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVST
CCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHH
IERLTAAGYVYIGMDHFALPNDDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVA
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHH
GHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFIIGELMCNGTLDIPAVEASYS
HHHHHCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
INFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAPAANDAKVIPLVRYSRVV
CCCCCCCCEEEEHHHHHCCC
>Mature Secondary Structure 
SAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCC
DAAYRRALDACRANVPAPLSLYVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMA
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHH
MAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKHFELLPEGEHAIEIDPRRVGD
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCH
DRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYG
HHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHCC
LPHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVST
CCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHH
IERLTAAGYVYIGMDHFALPNDDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVA
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHH
GHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFIIGELMCNGTLDIPAVEASYS
HHHHHCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
INFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAPAANDAKVIPLVRYSRVV
CCCCCCCCEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9396835 [H]