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The map label for this gene is dmlR [H]

Identifier: 94312377

GI number: 94312377

Start: 3721412

End: 3722407

Strand: Reverse

Name: dmlR [H]

Synonym: Rmet_3446

Alternate gene names: 94312377

Gene position: 3722407-3721412 (Counterclockwise)

Preceding gene: 94312378

Following gene: 94312374

Centisome position: 94.76

GC content: 64.06

Gene sequence:

>996_bases
ATGGGCGTCATAGCGCCCCGGGGATGCGCGATATGGCTCCGATGCCTCACAATGTGCCGGCAGGAGACATTTAGCTGGGC
TGGCATGGACAAACTCAAACAGATCGAAGCATTCATCGCCGTGGTGGAACACGGCAGCATGGCCGCCGCCGCGCTGACGC
AGAACGTCACGCCGGTCATGATCGGCCGGCGCATCAACGCGCTGGAGGCACGCATCGGCGTCAAGCTGCTGCATCGCTCC
ACGCGCCGCATTGTGGTGACCGAGCAGGGCGCCGCGTTCATGGAGCAGTGCAAGAAGGCGCTGGCCGACCTGGACCGCGC
CGAGATGCTGATTGCCGAAGGCAAGCACAAGGCCACCGGTCACCTGATCGTGTCGGCACCGGCCGCATTCGGGCGCAAGC
ACGTGGCGCCGCACGCGCCGGCCTTTCTCGCCGCCAATCCCGAGGTGCAGATTTCATTCAACCTGACCGACCGCGTGGTG
GATCTGGTGCGCGAGGGCTATGACGTCGGCATCCGCATCGGGGGCGCGATCGACCCGAACTTCGTGGCGATCAAGCTGGC
GACCAATAAGCGCGTGGTATGCGGCACGCCCGCGTACTTTGCCAGGCACGGCGTGCCGCACACGCTCGAGGATCTGGAGA
AGCACAACTGCCTCGCGTTCAACCTGCAAGGCGGGCAGCAGCGTGGCTGGTACTTCCAGCAAAACGGTAAGACCGTGACG
GTGCGCGTGAACGGCAATCTCGACTGCAACGACGGCGAGCTATTGCATCGCTGGACTGGCGAGAGCCTGGGACTGGGCTG
GCGTTCGACGTGGGAGATCCTGCCGCAACTGGAAAGTGGGGAGCTGATCACGGTACTCGACGAGTACGCGCTGCCGGATT
ACGACATCCTCGCGGTGTACCCGCAGCAGCGGCCGGTGCCGGCCAAGATCCGCTTCTTCATCGAGCACTTGAAACAGGCG
TTTGCAAAGCCGGGGTATTGGAGCGGCAGGGTCTGA

Upstream 100 bases:

>100_bases
TGGGTGAAGCAGCGGAAATCAACGAGGCCGTGAACCGCCAGCGTCAGCCGTAGCAGTCGTCCAGCACGGAGCTTCCGGGG
CGTGACGATTGGTGGGCCGG

Downstream 100 bases:

>100_bases
GGGGTTTGAAGCGTTGGTGCTATGGCGCCGTCAGCGCGGAACCAGATCCGCAAAAACCGAACCCCCAAAAGAAAAAGCCC
GCAGCCATTGCGGTCTGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MGVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVMIGRRINALEARIGVKLLHRS
TRRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVV
DLVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVT
VRVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKQA
FAKPGYWSGRV

Sequences:

>Translated_331_residues
MGVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVMIGRRINALEARIGVKLLHRS
TRRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVV
DLVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVT
VRVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKQA
FAKPGYWSGRV
>Mature_330_residues
GVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVMIGRRINALEARIGVKLLHRST
RRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVD
LVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVTV
RVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKQAF
AKPGYWSGRV

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=35.6164383561644, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=33.6666666666667, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=29.0102389078498, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=26.6211604095563, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.7397260273973, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.1168384879725, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=25.2525252525253, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789173, Length=207, Percent_Identity=27.0531400966184, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=161, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36716; Mature: 36585

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEE
IGRRINALEARIGVKLLHRSTRRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATG
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
HLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPN
EEEEECCHHHCCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHCCCEEEEEECCCCCCC
FVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVT
EEEEEEECCCEEEECCCHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCEEECCCCEEE
VRVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVY
EEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEEHHHCCCCCCEEEEC
PQQRPVPAKIRFFIEHLKQAFAKPGYWSGRV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVM
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEE
IGRRINALEARIGVKLLHRSTRRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATG
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
HLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPN
EEEEECCHHHCCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHCCCEEEEEECCCCCCC
FVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVT
EEEEEEECCCEEEECCCHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCEEECCCCEEE
VRVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVY
EEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEEHHHCCCCCCEEEEC
PQQRPVPAKIRFFIEHLKQAFAKPGYWSGRV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]