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The map label for this gene is amaB [H]

Identifier: 94312374

GI number: 94312374

Start: 3717368

End: 3718624

Strand: Reverse

Name: amaB [H]

Synonym: Rmet_3443

Alternate gene names: 94312374

Gene position: 3718624-3717368 (Counterclockwise)

Preceding gene: 94312377

Following gene: 94312373

Centisome position: 94.67

GC content: 67.78

Gene sequence:

>1257_bases
ATGGCAGCAACCCCTACGCCGTTGTCCGCTGAAACCGGCACGCGCATCATGACCTGGGCCGACGCCCTGGCCGTTCATAC
CGATCAGCCCGGCATCCTTACCCGCACCTATCTGACCGAGGCCCATCACGGCGCCGCCGCGCTGCTGACCGAATGGATGG
AAGCTGCGGGCATGACCGTGCGCCGCGATGCCGCCGGCAACGTGATCGGCCGCTATGAAGGCACCACGCCAAACGCCCCG
GCGCTGCTGACGGGTTCCCACTTCGACACCGTGCGCGACGCGGGTCGCTACGACGGCAACCTCGGCGTGATCCTGCCGAT
CGCCTGTGTGGCCGAGTGGAACCGCCAGGGCAAGCGCTTTCCGTTCGCGCTCGAAGTGGTCGGGTTTGCCGAGGAAGAGG
GCGTGCGCTTCAAGGCCACGCTGCTGGGCAGCCGCGCCATCGCGGGCACGTTCGATACCAATGTGCTCGACAACGTCGAT
GACTCGGGCAAGACCATGCGCGAAGTGATGCGCGCGGCCGGGTTCGATGCCGGGCAACTGCCCGCCGCGAAGCACGATCG
CAGCCAGGTGCTGGCCTTCATCGAGGTTCACATCGAGCAAGGTCCGGTGCTGCTGAACGAAGGCCTGCCCGTGGGAGTGG
TTACCGCCATCTCTGGCGCCACGCGCTTCATCGTCGAGCTGGAAGGCCTGGCCGGCCACGCCGGCACGGTGCCGATGGAC
ATGCGCCGCGACGCCGCGATGGCCGGCGCGGAGATCGGCCTCTTCATCGAGAAGCGCTGCGGCGGCAAGCCGGGCCTGGT
CGGCACGGTCGGCCAGTTCAATGTGCCGAACGGCGCCACCAATGTGGTTCCGGGCCGCGCGGTGTTTTCCATCGATATCC
GCGCTGGCGATGACGCCGAGCGCGAGGCCGCCGTCAACGACGTGCTGGCCGAAATCGAACGTGTTTGCGCGCGCCGCAAC
GTCCGCTCGCAGATCCGCAAGACGCACGAAGCCGCCAGCGTTCCGTGCGCGCCGTGGCTGCAGTCCCAATGGGCCGCGGC
AGTAGAGCGCCAGGGCGTGCCCGTGCATCACCTGCCCTCCGGCGCGGGCCATGACGCCATGGCCATTGCCGCCATCGCCG
ATGTGGCCATGCTGTTCGTTCGCTGCGGCAACGGCGGCATCAGCCATCACCCCACCGAAACCATGACGTCGGAAGACGCC
CAGACGGCCGCGCGTGTGTTCGCCGATTTCGTCGAGCACTTCCATCGCCCCCAATAA

Upstream 100 bases:

>100_bases
CACAGAATAGAGCTACGGCACCGGGCGCCTGCCTTGACTGGCCCTCCCGCCAGGATTTCCTCGACCGAGCTCACGCACCA
CCAGCAGGAGAGACACACAC

Downstream 100 bases:

>100_bases
GCGCCGCCATCGGCGTCGCCCCGAAAACTCCAGAGATAACGAAGATGACCGCACGAGACAACATGAATCCCGTTGAAACC
ACGCTGACGCAATATATCGA

Product: allantoate amidohydrolase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTVRRDAAGNVIGRYEGTTPNAP
ALLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRFPFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVD
DSGKTMREVMRAAGFDAGQLPAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMD
MRRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAEREAAVNDVLAEIERVCARRN
VRSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPSGAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDA
QTAARVFADFVEHFHRPQ

Sequences:

>Translated_418_residues
MAATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTVRRDAAGNVIGRYEGTTPNAP
ALLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRFPFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVD
DSGKTMREVMRAAGFDAGQLPAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMD
MRRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAEREAAVNDVLAEIERVCARRN
VRSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPSGAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDA
QTAARVFADFVEHFHRPQ
>Mature_417_residues
AATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTVRRDAAGNVIGRYEGTTPNAPA
LLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRFPFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVDD
SGKTMREVMRAAGFDAGQLPAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMDM
RRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAEREAAVNDVLAEIERVCARRNV
RSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPSGAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDAQ
TAARVFADFVEHFHRPQ

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=385, Percent_Identity=33.2467532467532, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 44416; Mature: 44285

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTV
CCCCCCCCCCCCCCEEEEEHHHHEEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCEE
RRDAAGNVIGRYEGTTPNAPALLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRF
EECCCCCEEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCC
PFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVDDSGKTMREVMRAAGFDAGQL
CEEEEEEEECCCCCCEEEEEEECCCEECCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCC
PAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMD
CCCCCCHHHEEEEEEEEECCCCEEEECCCCEEHHHHHCCCEEEEEEEECCCCCCCCCCHH
MRRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAE
HHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHH
REAAVNDVLAEIERVCARRNVRSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCC
GAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDAQTAARVFADFVEHFHRPQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTV
CCCCCCCCCCCCCEEEEEHHHHEEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCEE
RRDAAGNVIGRYEGTTPNAPALLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRF
EECCCCCEEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCC
PFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVDDSGKTMREVMRAAGFDAGQL
CEEEEEEEECCCCCCEEEEEEECCCEECCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCC
PAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMD
CCCCCCHHHEEEEEEEEECCCCEEEECCCCEEHHHHHCCCEEEEEEEECCCCCCCCCCHH
MRRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAE
HHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHH
REAAVNDVLAEIERVCARRNVRSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCC
GAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDAQTAARVFADFVEHFHRPQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]