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The map label for this gene is aroK [H]

Identifier: 94312198

GI number: 94312198

Start: 3541717

End: 3542406

Strand: Reverse

Name: aroK [H]

Synonym: Rmet_3267

Alternate gene names: 94312198

Gene position: 3542406-3541717 (Counterclockwise)

Preceding gene: 94312199

Following gene: 94312197

Centisome position: 90.18

GC content: 62.75

Gene sequence:

>690_bases
ATGATGCACAATGGCGTATGCATCACGCCCCTTGCGGGTTCCCGGCCTGACGCGGGTGCCCGCCGCCTTATCGTGCAAGA
CCTCAGTCCAGTGACAGCCAATCGCCCGAATATCTTCTTTGTTGGCCTGATGGGCGCCGGCAAGACCACCGTCGGCCGCA
CCGTGGCCCGTCGTCTCAACTATCCGTTCTTCGATTCCGATCATGAACTTGAGGCGCGCTGCGGCGTACGAATTCCGATA
ATTTTCGAGCATGAGGGCGAAGCGGGGTTCCGCGATCGCGAGGCCCAGGTGATTGCCGAGCTGACCGGGCGCAGTGGCAT
CGTTCTGGCCACAGGCGGCGGCGCGGTGCTCCGCGAGGTCAACCGCGCGGCGTTGAAGGCCAATGGCACTGTGGTCTATC
TGCGCGCCAGCCCGCACGATCTGTGGCTGCGCACGCGGCATGACCGTAACCGACCGCTCCTGCAGACGGAGGATCCGAAG
GGTCGCCTCGAGGCACTCTATGCCGAGCGCGATCCGCTCTATCGCGAGGTGGCGGACTTCATTATCGAAACCGGCAAGCC
GACCGTGGCGCAGCTTGCTAATATGGTTCTTATGCAACTTGAAATGGCCGGCTTTGTGTTTCCGCCCGCGGAAATCGTGC
CCGAATCTGCCGAGGATGGTGGGCCGCCGGCGCAGGACACCCATTCATGA

Upstream 100 bases:

>100_bases
TGCAGTAGACTGGCAGGCCGCGTCGCAAAACGACGCGGCCTTTTTCTATTTGGCGGCAACGTTGGCGGCTCTGAGGGTCG
GCCAGGACCAGCGCGGCGGG

Downstream 100 bases:

>100_bases
TCACGCTTGAAGTCGATCTCGGGACGCGTAGTTACCCGATCCACATTGGCAGCGGCCTGCTGGACCGCGCCGACCTGCTG
GCCCCGCATGTACGCGGCCA

Product: shikimate kinase

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLNYPFFDSDHELEARCGVRIPI
IFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREVNRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPK
GRLEALYAERDPLYREVADFIIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS

Sequences:

>Translated_229_residues
MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLNYPFFDSDHELEARCGVRIPI
IFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREVNRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPK
GRLEALYAERDPLYREVADFIIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS
>Mature_229_residues
MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLNYPFFDSDHELEARCGVRIPI
IFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREVNRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPK
GRLEALYAERDPLYREVADFIIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=170, Percent_Identity=50, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1786587, Length=155, Percent_Identity=40, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 25063; Mature: 25063

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01128 SHIKIMATE_KINASE ; PS00267 TACHYKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLN
CCCCCEEEEECCCCCCCCHHHEEHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
YPFFDSDHELEARCGVRIPIIFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREV
CCCCCCCCCCHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHH
NRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGRLEALYAERDPLYREVADF
HHHHEECCCEEEEEEECCCEEEEEECCCCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHH
IIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLN
CCCCCEEEEECCCCCCCCHHHEEHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
YPFFDSDHELEARCGVRIPIIFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREV
CCCCCCCCCCHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHH
NRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGRLEALYAERDPLYREVADF
HHHHEECCCEEEEEEECCCEEEEEECCCCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHH
IIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA