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The map label for this gene is pilQ [H]

Identifier: 94312199

GI number: 94312199

Start: 3542536

End: 3544668

Strand: Reverse

Name: pilQ [H]

Synonym: Rmet_3268

Alternate gene names: 94312199

Gene position: 3544668-3542536 (Counterclockwise)

Preceding gene: 94312200

Following gene: 94312198

Centisome position: 90.24

GC content: 62.45

Gene sequence:

>2133_bases
ATGATCACGGCACGATGGGTCAAGGCGCTCGCGGGCGCGGCAACGATGGTGCTGGTGCTCGCCTCTTCGGCGGCGCTGGC
GCAGAACAACCAGGTCAAGCAGATGGAAGCCAACGTGCTTGGCGAGCAGACCGTATTTACGGTCGATCTGCAGGGACCAT
TGGCGCAGAAACCAGCGGACTTCACCACGCAGAATCCGGCGAAGATCGCGATCGACTTCTTCGACACGGGCTTCGCGGCG
GGGCGCGCCCAGTACGAGTACGGCGGCAAGGTGCTCAAGAGCGCCAACGTGATGCAGATTGGGGACCGCACACGCGTGGT
GCTGAACCTGAGCCGGTCGACAACATATCGCACCGAGGTGCGTGGCAACCAGTTCGTGGTGATGCTGGACAACGTCCAGC
AGGCCGCGACCGGCGCGGTGCCGACGTTCGCGCCGGCGACGACGGTGCCGACATCGGCGATGGCAGCGAATCGTCCGTCG
ATTCGGAACATCGATTTCCGTCGCGGGGGCGATGGCGCCGGGCGTGTGGTGGTGGATCTCTCGTCGCGTGATTCAGGCAT
CAACATCGCGCAGCAGGGCCAGAACCTCGTGGTCGACTTCATGGGCACGTCGGTGCCGGCGGATCTGCGCCGCCGCTTCG
ATGTGACCGATTTCGGCACGCCAATCCAGAGCATGCGCGCGAGCGATGCCAATGGCAGCGCGAAACTGACGATCGAGCCG
CGTGGCAACTGGGAGTACAGCTCGTACCAGACGGACAACCAGTTCGTGGTGGAGGTGCGGCAGGTCAAGGATGACCCGAA
CAAGCTGATCGCGGGCCAAGGCTTCCGCGGCGAGCGGCTATCCCTGAACTTCCAGAACATCGACATCCGCTCGCTGCTGC
AGGTGTTCGCCGACTTCACGAACCTGAACATCATCACGAGCGACACGGTCCAGGGCAACCTGACGCTGCGCCTGAAGGAT
GTGCCGTGGGACCAGGCGCTGCAGATTGTGATGGATTCGAAGGGCCTGGCCTCGCGCCGCAACGGCAATGTGCTGTGGGT
GGCGCCCAAGGCGGAACTGGCGACCAAGGAGAAGCTTGAACTCGAGGCGATGCAGCAGATCAACGAACTCGAGCCGATCC
GCAGCCAGGTGTTCCAGCTCAACTACCAGCGTGCCGACGACGTTCGCAAGATGCTGCTCGGCATGGTTACAGGCACTGCC
GGGGCGGCAGGTGCGATGCCGGTGCCGGTTGGTGGCGCAGCAGGCATGATGGGCGCGACGCGGATGCTGTCCAAACGCGG
TTCGCTGACGGCGGATCCCCGCACGAACCAGCTGTTCGTGTCCGATGTATCGAGCAAGCTGGAGGAAGTGCAGATCTTCC
TGCAGAAGATCGATATCCCGGTTCGGCAGGTGATCATCGAGGCACGCATCGTTGAGGCTAGCGATACCTTCAGCCGGAAC
CTCGGTGTGAAGCTCGGCTTTGCGTTGCCGAAGTCCGGCAGCTCGCTGAGCAGCAACTACGGTAACTCGCTGCCGAATGC
CACGAACGACGGTACCCCGGCACTGAGCCTGCCGGCGGCAGCCATCAACAGCGTGAACCCGGCTACGGCGGCATTCAGTA
TCTTCAACGCCGCCGCGGGCCGATTCCTGGATCTGGAACTGTCCGCACTGGAAGCCGACGGCAAGGGCAAGATCATCTCG
AGCCCGCGCGTCGTGACGGCCAACAACATCAAGGCACTGATCGAGCAGGGTACCGAACTGCCGTACCAGTCGGCCACCTC
AAGCGGCGCGACCTCGGTGCAGTTCCGCAAGGCCAACCTGAAGCTTGAAGTCACGCCGCAGATCACGCCGGAAGGCAATG
TGCTGCTGGACGTCGACGTGAACAAGGACAGCGTCGGTATCCGAACGGACAACGGCTTCGCCATCGACACCAAGCACGTG
CAGACGCAGGTGCTGGTGGAGAACGGCGGCACGGTCGTAATCGGCGGTATCTATAGCCAGGAAGAGTCCAATACCGTGAA
CAAGGTACCTTGGCTGGGCGACCTCCCGGTGCTTGGCAACTTCTTCAAGAACAACGCCAAGGTCAACAACCGGACCGAAC
TGCTGGTGTTCCTGACGCCGCGTGTCCTGAACGATAACGTGTCGCTCAAATAA

Upstream 100 bases:

>100_bases
TCCGCATCACCGATCAGGAGATCGTGCTGCGCGAATTGGTCCGGGAAGGGGTCTCAGAGTGGAAAGAGAAAATGACCAGC
CTGAAGCTGGAGGCATCGGC

Downstream 100 bases:

>100_bases
CGCCGGCCGGGTGCAGCAGGGGGCCCGGATGCAGTAGACTGGCAGGCCGCGTCGCAAAACGACGCGGCCTTTTTCTATTT
GGCGGCAACGTTGGCGGCTC

Product: pili assembly protein PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MITARWVKALAGAATMVLVLASSAALAQNNQVKQMEANVLGEQTVFTVDLQGPLAQKPADFTTQNPAKIAIDFFDTGFAA
GRAQYEYGGKVLKSANVMQIGDRTRVVLNLSRSTTYRTEVRGNQFVVMLDNVQQAATGAVPTFAPATTVPTSAMAANRPS
IRNIDFRRGGDGAGRVVVDLSSRDSGINIAQQGQNLVVDFMGTSVPADLRRRFDVTDFGTPIQSMRASDANGSAKLTIEP
RGNWEYSSYQTDNQFVVEVRQVKDDPNKLIAGQGFRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNLTLRLKD
VPWDQALQIVMDSKGLASRRNGNVLWVAPKAELATKEKLELEAMQQINELEPIRSQVFQLNYQRADDVRKMLLGMVTGTA
GAAGAMPVPVGGAAGMMGATRMLSKRGSLTADPRTNQLFVSDVSSKLEEVQIFLQKIDIPVRQVIIEARIVEASDTFSRN
LGVKLGFALPKSGSSLSSNYGNSLPNATNDGTPALSLPAAAINSVNPATAAFSIFNAAAGRFLDLELSALEADGKGKIIS
SPRVVTANNIKALIEQGTELPYQSATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIRTDNGFAIDTKHV
QTQVLVENGGTVVIGGIYSQEESNTVNKVPWLGDLPVLGNFFKNNAKVNNRTELLVFLTPRVLNDNVSLK

Sequences:

>Translated_710_residues
MITARWVKALAGAATMVLVLASSAALAQNNQVKQMEANVLGEQTVFTVDLQGPLAQKPADFTTQNPAKIAIDFFDTGFAA
GRAQYEYGGKVLKSANVMQIGDRTRVVLNLSRSTTYRTEVRGNQFVVMLDNVQQAATGAVPTFAPATTVPTSAMAANRPS
IRNIDFRRGGDGAGRVVVDLSSRDSGINIAQQGQNLVVDFMGTSVPADLRRRFDVTDFGTPIQSMRASDANGSAKLTIEP
RGNWEYSSYQTDNQFVVEVRQVKDDPNKLIAGQGFRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNLTLRLKD
VPWDQALQIVMDSKGLASRRNGNVLWVAPKAELATKEKLELEAMQQINELEPIRSQVFQLNYQRADDVRKMLLGMVTGTA
GAAGAMPVPVGGAAGMMGATRMLSKRGSLTADPRTNQLFVSDVSSKLEEVQIFLQKIDIPVRQVIIEARIVEASDTFSRN
LGVKLGFALPKSGSSLSSNYGNSLPNATNDGTPALSLPAAAINSVNPATAAFSIFNAAAGRFLDLELSALEADGKGKIIS
SPRVVTANNIKALIEQGTELPYQSATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIRTDNGFAIDTKHV
QTQVLVENGGTVVIGGIYSQEESNTVNKVPWLGDLPVLGNFFKNNAKVNNRTELLVFLTPRVLNDNVSLK
>Mature_710_residues
MITARWVKALAGAATMVLVLASSAALAQNNQVKQMEANVLGEQTVFTVDLQGPLAQKPADFTTQNPAKIAIDFFDTGFAA
GRAQYEYGGKVLKSANVMQIGDRTRVVLNLSRSTTYRTEVRGNQFVVMLDNVQQAATGAVPTFAPATTVPTSAMAANRPS
IRNIDFRRGGDGAGRVVVDLSSRDSGINIAQQGQNLVVDFMGTSVPADLRRRFDVTDFGTPIQSMRASDANGSAKLTIEP
RGNWEYSSYQTDNQFVVEVRQVKDDPNKLIAGQGFRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNLTLRLKD
VPWDQALQIVMDSKGLASRRNGNVLWVAPKAELATKEKLELEAMQQINELEPIRSQVFQLNYQRADDVRKMLLGMVTGTA
GAAGAMPVPVGGAAGMMGATRMLSKRGSLTADPRTNQLFVSDVSSKLEEVQIFLQKIDIPVRQVIIEARIVEASDTFSRN
LGVKLGFALPKSGSSLSSNYGNSLPNATNDGTPALSLPAAAINSVNPATAAFSIFNAAAGRFLDLELSALEADGKGKIIS
SPRVVTANNIKALIEQGTELPYQSATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIRTDNGFAIDTKHV
QTQVLVENGGTVVIGGIYSQEESNTVNKVPWLGDLPVLGNFFKNNAKVNNRTELLVFLTPRVLNDNVSLK

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=449, Percent_Identity=32.293986636971, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082242, Length=388, Percent_Identity=29.1237113402062, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 76511; Mature: 76511

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITARWVKALAGAATMVLVLASSAALAQNNQVKQMEANVLGEQTVFTVDLQGPLAQKPAD
CCCHHHHHHHHHHHHEEHHHHCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
FTTQNPAKIAIDFFDTGFAAGRAQYEYGGKVLKSANVMQIGDRTRVVLNLSRSTTYRTEV
CCCCCCCEEEEEEECCCCCCCCCCHHCCCCEECCCCEEEECCCEEEEEEECCCCEEEEEE
RGNQFVVMLDNVQQAATGAVPTFAPATTVPTSAMAANRPSIRNIDFRRGGDGAGRVVVDL
CCCEEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
SSRDSGINIAQQGQNLVVDFMGTSVPADLRRRFDVTDFGTPIQSMRASDANGSAKLTIEP
CCCCCCCCHHHCCCEEEEEECCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEEEEC
RGNWEYSSYQTDNQFVVEVRQVKDDPNKLIAGQGFRGERLSLNFQNIDIRSLLQVFADFT
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHC
NLNIITSDTVQGNLTLRLKDVPWDQALQIVMDSKGLASRRNGNVLWVAPKAELATKEKLE
CCEEEEECCEECEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEECCHHHCCHHHHH
LEAMQQINELEPIRSQVFQLNYQRADDVRKMLLGMVTGTAGAAGAMPVPVGGAAGMMGAT
HHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
RMLSKRGSLTADPRTNQLFVSDVSSKLEEVQIFLQKIDIPVRQVIIEARIVEASDTFSRN
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCHHCC
LGVKLGFALPKSGSSLSSNYGNSLPNATNDGTPALSLPAAAINSVNPATAAFSIFNAAAG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHCCCCCHHHHHHHHHHCCC
RFLDLELSALEADGKGKIISSPRVVTANNIKALIEQGTELPYQSATSSGATSVQFRKANL
CEEEEEEEEEECCCCCCEECCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCE
KLEVTPQITPEGNVLLDVDVNKDSVGIRTDNGFAIDTKHVQTQVLVENGGTVVIGGIYSQ
EEEECCEECCCCCEEEEEECCCCCCEEECCCCEEEECCCCEEEEEEECCCEEEEEEEECC
EESNTVNKVPWLGDLPVLGNFFKNNAKVNNRTELLVFLTPRVLNDNVSLK
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCC
>Mature Secondary Structure
MITARWVKALAGAATMVLVLASSAALAQNNQVKQMEANVLGEQTVFTVDLQGPLAQKPAD
CCCHHHHHHHHHHHHEEHHHHCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
FTTQNPAKIAIDFFDTGFAAGRAQYEYGGKVLKSANVMQIGDRTRVVLNLSRSTTYRTEV
CCCCCCCEEEEEEECCCCCCCCCCHHCCCCEECCCCEEEECCCEEEEEEECCCCEEEEEE
RGNQFVVMLDNVQQAATGAVPTFAPATTVPTSAMAANRPSIRNIDFRRGGDGAGRVVVDL
CCCEEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
SSRDSGINIAQQGQNLVVDFMGTSVPADLRRRFDVTDFGTPIQSMRASDANGSAKLTIEP
CCCCCCCCHHHCCCEEEEEECCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEEEEC
RGNWEYSSYQTDNQFVVEVRQVKDDPNKLIAGQGFRGERLSLNFQNIDIRSLLQVFADFT
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHC
NLNIITSDTVQGNLTLRLKDVPWDQALQIVMDSKGLASRRNGNVLWVAPKAELATKEKLE
CCEEEEECCEECEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEECCHHHCCHHHHH
LEAMQQINELEPIRSQVFQLNYQRADDVRKMLLGMVTGTAGAAGAMPVPVGGAAGMMGAT
HHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
RMLSKRGSLTADPRTNQLFVSDVSSKLEEVQIFLQKIDIPVRQVIIEARIVEASDTFSRN
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCHHCC
LGVKLGFALPKSGSSLSSNYGNSLPNATNDGTPALSLPAAAINSVNPATAAFSIFNAAAG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHCCCCCHHHHHHHHHHCCC
RFLDLELSALEADGKGKIISSPRVVTANNIKALIEQGTELPYQSATSSGATSVQFRKANL
CEEEEEEEEEECCCCCCEECCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCE
KLEVTPQITPEGNVLLDVDVNKDSVGIRTDNGFAIDTKHVQTQVLVENGGTVVIGGIYSQ
EEEECCEECCCCCEEEEEECCCCCCEEECCCCEEEECCCCEEEEEEECCCEEEEEEEECC
EESNTVNKVPWLGDLPVLGNFFKNNAKVNNRTELLVFLTPRVLNDNVSLK
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]