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The map label for this gene is mlaA [H]

Identifier: 94312187

GI number: 94312187

Start: 3526948

End: 3527748

Strand: Reverse

Name: mlaA [H]

Synonym: Rmet_3256

Alternate gene names: 94312187

Gene position: 3527748-3526948 (Counterclockwise)

Preceding gene: 94312188

Following gene: 94312186

Centisome position: 89.81

GC content: 65.92

Gene sequence:

>801_bases
ATGATGGTCTTGCAGCGCCTGGCCGTTGCATCGCTGGCGGCGCTTGCCCTGGCGGGTTGCGCAACGGGCCCGAACGCGAA
TCCCGCGGATCCGCTCGAGCCGTTCAACCGCGGCGTCAGCACGTTCAACGAGAAGCTCGACACCTATGCACTGAAGCCGG
TGGCCACGGTCTATTCGGACTACACGCCGACCCCGGTGCAAATTGCGGTCGGCAATTTCTTCTCGAATGTCAGTGACGTC
TACTCCGCGGCGAATAATCTGCTGCAAGGCAAGCCGTCGCGCGCGGCCGAGGACACGATGCGCGTGGCGATCAACAGTGT
GCTCGGCATCGGGGGGCTGATCGACATCGCCACGCCGGCCGGCCTGCCGAAGTACAAGGAAGACTTCGGCCAGACCATGG
GCGTGTGGGGTGTGCCTGCGGGGCCGTACCTGGTGCTGCCGCTGTTCGGGCCGAGCAGCGTGCGGGATACCGCAGGCATG
ATCGTGGACCGCTTCTCGGATCCGACCACCTATATCTCGCCCTGGTATGCCGGCTTGGGCCTGCAAACCGTCCGCGTGAT
CGACACGCGCGCGCAATTGCTTGGCGCGTCGAGCCTGATCGAGGCGGCGGCGCTGGACCGCTATTCGTTCCTGCGCGATT
CATACCTGCAGCGGCGCAACTACCTGATCCACGACGGTGAGGTGCCGGACAAGGATGGTGGCGCGCCGGACGGAAAGCAG
GACGACAACAGCGCGCCCGCGCAGCCATCCGCACCGGAGCCCGCCACGCCCACGACCCCAGCAAGTCCGAAGGCGGGTTG
A

Upstream 100 bases:

>100_bases
ACAACAAGGCGGCCGATGCCGGCGCTGGTGGCGGATCGACCTCGTCAGGCTCGGGTGCCGGAGCGCTGCCTGCAGCGCCC
GGTACTGGCGGGAGCGGCAA

Downstream 100 bases:

>100_bases
TTGCCCCATCAGGCCAGTGCTGACAACGCCTGTGTCGCCTGTGAGCACGCTGTGAGCCCTGCAACAAAGTGTTTGAATGT
GGTGTTTGGAACATTTCGCG

Product: VacJ-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMVLQRLAVASLAALALAGCATGPNANPADPLEPFNRGVSTFNEKLDTYALKPVATVYSDYTPTPVQIAVGNFFSNVSDV
YSAANNLLQGKPSRAAEDTMRVAINSVLGIGGLIDIATPAGLPKYKEDFGQTMGVWGVPAGPYLVLPLFGPSSVRDTAGM
IVDRFSDPTTYISPWYAGLGLQTVRVIDTRAQLLGASSLIEAAALDRYSFLRDSYLQRRNYLIHDGEVPDKDGGAPDGKQ
DDNSAPAQPSAPEPATPTTPASPKAG

Sequences:

>Translated_266_residues
MMVLQRLAVASLAALALAGCATGPNANPADPLEPFNRGVSTFNEKLDTYALKPVATVYSDYTPTPVQIAVGNFFSNVSDV
YSAANNLLQGKPSRAAEDTMRVAINSVLGIGGLIDIATPAGLPKYKEDFGQTMGVWGVPAGPYLVLPLFGPSSVRDTAGM
IVDRFSDPTTYISPWYAGLGLQTVRVIDTRAQLLGASSLIEAAALDRYSFLRDSYLQRRNYLIHDGEVPDKDGGAPDGKQ
DDNSAPAQPSAPEPATPTTPASPKAG
>Mature_266_residues
MMVLQRLAVASLAALALAGCATGPNANPADPLEPFNRGVSTFNEKLDTYALKPVATVYSDYTPTPVQIAVGNFFSNVSDV
YSAANNLLQGKPSRAAEDTMRVAINSVLGIGGLIDIATPAGLPKYKEDFGQTMGVWGVPAGPYLVLPLFGPSSVRDTAGM
IVDRFSDPTTYISPWYAGLGLQTVRVIDTRAQLLGASSLIEAAALDRYSFLRDSYLQRRNYLIHDGEVPDKDGGAPDGKQ
DDNSAPAQPSAPEPATPTTPASPKAG

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=244, Percent_Identity=34.8360655737705, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28032; Mature: 28032

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVLQRLAVASLAALALAGCATGPNANPADPLEPFNRGVSTFNEKLDTYALKPVATVYSD
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YTPTPVQIAVGNFFSNVSDVYSAANNLLQGKPSRAAEDTMRVAINSVLGIGGLIDIATPA
CCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
GLPKYKEDFGQTMGVWGVPAGPYLVLPLFGPSSVRDTAGMIVDRFSDPTTYISPWYAGLG
CCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCC
LQTVRVIDTRAQLLGASSLIEAAALDRYSFLRDSYLQRRNYLIHDGEVPDKDGGAPDGKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
DDNSAPAQPSAPEPATPTTPASPKAG
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMVLQRLAVASLAALALAGCATGPNANPADPLEPFNRGVSTFNEKLDTYALKPVATVYSD
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YTPTPVQIAVGNFFSNVSDVYSAANNLLQGKPSRAAEDTMRVAINSVLGIGGLIDIATPA
CCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
GLPKYKEDFGQTMGVWGVPAGPYLVLPLFGPSSVRDTAGMIVDRFSDPTTYISPWYAGLG
CCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCC
LQTVRVIDTRAQLLGASSLIEAAALDRYSFLRDSYLQRRNYLIHDGEVPDKDGGAPDGKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
DDNSAPAQPSAPEPATPTTPASPKAG
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]