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The map label for this gene is hisF [H]

Identifier: 94312173

GI number: 94312173

Start: 3515139

End: 3515909

Strand: Reverse

Name: hisF [H]

Synonym: Rmet_3242

Alternate gene names: 94312173

Gene position: 3515909-3515139 (Counterclockwise)

Preceding gene: 94312174

Following gene: 94312172

Centisome position: 89.51

GC content: 65.11

Gene sequence:

>771_bases
ATGTTAGCCAAACGTATCATCCCCTGCCTGGACGTGACCAATGGGCGGGTGGTCAAGGGTGTCAATTTCGTCGAGCTGCG
CGACGCGGGCGATCCCGTCGAGATCGCGCGGCGCTACGACGAGCAGGGTGCTGATGAAATCACATTTCTCGACATCACGG
CAACCAGCGACGGCCGCGACCTGATCCTGCACATCATCGAGGCGGTGGCGTCGCAGGTGTTCATACCGCTGACCGTCGGG
GGTGGCGTGCGCACTGTGGAAGATGTGCGGCGACTGCTGAATGCAGGCGCAGACAAGATCAGCGTGAACTCGTCGGCCAT
CGCCAATCCGCAGCTCGTGTCCGACGCAACGGGCAAGTACGGCTCGCAGTGCATCGTTGTGGCCATCGACGCCAAGCGCA
GCTCCGCGCCCGGCGAACCGCCGCGCTGGGAAGTGTTCACTCACGGCGGCCGCAAGGCCACCGGACTCGATGCGGTGGAG
TGGGCCCGCGAGATGGCACGGCGCGGCGCGGGCGAGATTCTGCTGACCAGCATGGATCGCGATGGCACGAAGAGCGGGTT
CGACCTCGAACTCACGCGTGCTGTCAGCGACGCGGTGCCCGTGCCCGTTATTGCATCTGGCGGGGTCGGTGGCCTGCAGG
ATCTGGCCGATGGCATCCGCCTGGGACACGCCGACGCCGTACTGGCCGCCAGCATCTTCCACTATGGCGAGCACACCGTT
GGTGAGGCAAAGGCTTTCATGGCCCGCGAGGGCATTCCCGTCCGGATGTGA

Upstream 100 bases:

>100_bases
GCGGCCCAGGCCCACGCGGACAAGCTGGGCGCGGAGTAATCCAAGCTCGCGCGCCACAACCAGCCGTCGCCGATGCGGGC
GACGGCGGGCAAGGAAACTC

Downstream 100 bases:

>100_bases
TCATGGCGAAGAAGTGGTTGAACAAGGTGAAGTGGGACGACAACGGTCTGGTGCCGGTGATCGTCCAGGAAGTCGGTTCC
AACGACGTCCTGATGTTCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGEHTV
GEAKAFMAREGIPVRM

Sequences:

>Translated_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGEHTV
GEAKAFMAREGIPVRM
>Mature_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGEHTV
GEAKAFMAREGIPVRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=43.4615384615385, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=30.327868852459, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.3503184713376, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27127; Mature: 27127

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
GSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVEWAREMARRGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGEHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
GEAKAFMAREGIPVRM
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
GSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVEWAREMARRGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGEHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
GEAKAFMAREGIPVRM
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA