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The map label for this gene is petB [H]

Identifier: 94312160

GI number: 94312160

Start: 3505176

End: 3506579

Strand: Reverse

Name: petB [H]

Synonym: Rmet_3229

Alternate gene names: 94312160

Gene position: 3506579-3505176 (Counterclockwise)

Preceding gene: 94312161

Following gene: 94312159

Centisome position: 89.27

GC content: 60.83

Gene sequence:

>1404_bases
ATGGCGGCCGAAAAAGAAGTCAAGACTACCGGGCTGCTGGGTTGGATCGACGCCCGCTTCCCAGCCACCCAACTTTGGGA
AGACCACCTCTCCAAGTACTACGCGCCGAAGAACTTCAACTTCTGGTACTTCTTTGGTTCGCTCGCACTGCTGGTGCTGG
TGATCCAGATCGTCACCGGCATCTTCCTGGTGATGAACTACAAGCCGGACGGCACGCTGAACGCGGCTGGCATTCCAGTG
GCATTCGCCAGCGTGGAATTCATCATGCGCGAAGTGCCATGGGGCTGGCTGATCCGCTACATGCACTCCACCGGCGCTTC
GGCGTTCTTCGTCGTGGTGTACCTGCACATGTTCCGGGGGCTGCTGTACGGTTCGTACCGAAAGCCGCGCGAACTGGTCT
GGATCTTCGGCTGCCTGATCTTCCTGTGCCTGATGGCCGAAGCGTTCATGGGCTACCTGCTGCCCTGGGGCCAGATGTCC
TATTGGGGCGCGCAGGTGATCGTGAACCTGTTCTCGGCGATTCCGGTGATCGGCCAGGACCTGTCGCTGTTCATCCGCGG
TGACTACGTGGTGAGCGATGCCACGCTGAACCGATTCTTCTCCTTCCACGTGATCGCGGTGCCGCTGGTGCTGCTGGGCC
TGGTGGTGGCGCACATCATCGCGCTGCACGAAGTGGGCTCGAACAACCCGGACGGCGTGGAAATCAAGGCGAAGAAGGAT
GAAAACGGCATTCCGCTCGATGGCATCCCGTTCCACCCGTACTACTCGGTGCATGACCTGCTGGGCGTGGCCGGCTTCCT
GATCATCTTCTCGGCCGTGATCTTCTTCTTCCCGGAGGTGGGCGGCTATTTCCTGGAAGCCAACAACTTCTTCCCGGCGG
ATCCGTTGAAGACACCGCCGCACATCGCGCCGGTGTGGTACTTCACGCCGTTCTACTCGATGCTGCGCGCAACCACGGCG
AACTTCCTGCCGATCCTGTGGGCGTTCTTCGCCATCGTGCTGGGCATGGTGTTCCTGCGCACCAAGGATGCACGCATCAA
GATGGGTTCGGTGGCGATCCTGGTGATCCTGGCCGTGGGCTTCTATTTCATCGACGCCAAGTTCTGGGGCGTGCTGGTGA
TGGGCGGATCGGTGATCGTGCTGTTCTTCATGCCGTGGCTCGATTGCTCGCCGGTCAAGTCCATCCGCTACCGTCCCGCA
TTCCACAAGACGATCCTGGTTGTCTTCGTGGTGGTGTTCCTGATCCTCGGCTACCTCGGCGTGCAACCGCCGTCGCCGGT
GGGTGAGAAGGTGTCGCAGCTCGGTACGCTGCTGTACTTCGCCTTCTTCCTGACCATGCCGCTGTGGAGCCGTGCCGGCA
CGTTCAAGCCGGTGCCGGAGCGCGTCACGTTCCATCCGCACTAA

Upstream 100 bases:

>100_bases
AGAACAAACCGGCCCCGCAGAACCTTGACGTACCCCCGTACCAGTTCCTGACCGACACCAAGCTCGTGATCGGCAAGGAC
GAGAAAGGAGAAGCCTGATC

Downstream 100 bases:

>100_bases
GACGCTGCGCGACCCTACAAGAGGACAAGGACACAAGATGAAAAAGTTGCTTTCGATCTTCGCACTGGTCGGCGCTTGTT
TCGCGGCAATGCCCGCGATG

Product: cytochrome b subunit, Ubiquinol-cytochrome-c reductase

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAAEKEVKTTGLLGWIDARFPATQLWEDHLSKYYAPKNFNFWYFFGSLALLVLVIQIVTGIFLVMNYKPDGTLNAAGIPV
AFASVEFIMREVPWGWLIRYMHSTGASAFFVVVYLHMFRGLLYGSYRKPRELVWIFGCLIFLCLMAEAFMGYLLPWGQMS
YWGAQVIVNLFSAIPVIGQDLSLFIRGDYVVSDATLNRFFSFHVIAVPLVLLGLVVAHIIALHEVGSNNPDGVEIKAKKD
ENGIPLDGIPFHPYYSVHDLLGVAGFLIIFSAVIFFFPEVGGYFLEANNFFPADPLKTPPHIAPVWYFTPFYSMLRATTA
NFLPILWAFFAIVLGMVFLRTKDARIKMGSVAILVILAVGFYFIDAKFWGVLVMGGSVIVLFFMPWLDCSPVKSIRYRPA
FHKTILVVFVVVFLILGYLGVQPPSPVGEKVSQLGTLLYFAFFLTMPLWSRAGTFKPVPERVTFHPH

Sequences:

>Translated_467_residues
MAAEKEVKTTGLLGWIDARFPATQLWEDHLSKYYAPKNFNFWYFFGSLALLVLVIQIVTGIFLVMNYKPDGTLNAAGIPV
AFASVEFIMREVPWGWLIRYMHSTGASAFFVVVYLHMFRGLLYGSYRKPRELVWIFGCLIFLCLMAEAFMGYLLPWGQMS
YWGAQVIVNLFSAIPVIGQDLSLFIRGDYVVSDATLNRFFSFHVIAVPLVLLGLVVAHIIALHEVGSNNPDGVEIKAKKD
ENGIPLDGIPFHPYYSVHDLLGVAGFLIIFSAVIFFFPEVGGYFLEANNFFPADPLKTPPHIAPVWYFTPFYSMLRATTA
NFLPILWAFFAIVLGMVFLRTKDARIKMGSVAILVILAVGFYFIDAKFWGVLVMGGSVIVLFFMPWLDCSPVKSIRYRPA
FHKTILVVFVVVFLILGYLGVQPPSPVGEKVSQLGTLLYFAFFLTMPLWSRAGTFKPVPERVTFHPH
>Mature_466_residues
AAEKEVKTTGLLGWIDARFPATQLWEDHLSKYYAPKNFNFWYFFGSLALLVLVIQIVTGIFLVMNYKPDGTLNAAGIPVA
FASVEFIMREVPWGWLIRYMHSTGASAFFVVVYLHMFRGLLYGSYRKPRELVWIFGCLIFLCLMAEAFMGYLLPWGQMSY
WGAQVIVNLFSAIPVIGQDLSLFIRGDYVVSDATLNRFFSFHVIAVPLVLLGLVVAHIIALHEVGSNNPDGVEIKAKKDE
NGIPLDGIPFHPYYSVHDLLGVAGFLIIFSAVIFFFPEVGGYFLEANNFFPADPLKTPPHIAPVWYFTPFYSMLRATTAN
FLPILWAFFAIVLGMVFLRTKDARIKMGSVAILVILAVGFYFIDAKFWGVLVMGGSVIVLFFMPWLDCSPVKSIRYRPAF
HKTILVVFVVVFLILGYLGVQPPSPVGEKVSQLGTLLYFAFFLTMPLWSRAGTFKPVPERVTFHPH

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=412, Percent_Identity=37.378640776699, Blast_Score=238, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6226529, Length=319, Percent_Identity=41.692789968652, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6226532, Length=248, Percent_Identity=44.758064516129, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6226531, Length=159, Percent_Identity=49.685534591195, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6226530, Length=144, Percent_Identity=47.9166666666667, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 52650; Mature: 52519

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAEKEVKTTGLLGWIDARFPATQLWEDHLSKYYAPKNFNFWYFFGSLALLVLVIQIVTG
CCCHHHHHHCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA