Click here to switch to the map view.

The map label for this gene is nirE [H]

Identifier: 94312099

GI number: 94312099

Start: 3440951

End: 3441808

Strand: Reverse

Name: nirE [H]

Synonym: Rmet_3168

Alternate gene names: 94312099

Gene position: 3441808-3440951 (Counterclockwise)

Preceding gene: 94312100

Following gene: 335055653

Centisome position: 87.62

GC content: 63.75

Gene sequence:

>858_bases
ATGCAAACGACCGGAAAGGTCTGGCTGGTTGGGGTGGGCCCGGGCGATCCGGAACTCGCCACCATGAAGGCAGTCAGGGC
GCTACGTGAAGCGGATGTCTGGCTAGTGGACGATCTCGTGCCGGAGGCCATGCTGTGCTATGCGAACCCCGGGACGCAAA
TCATCTCGGTCGGCAAGCGTGGCGGCCGCTGTTCGGTGAGTCAATCCAGCATTCACGAGCAGGCGCTCGAGCACGCGCAG
GCTGGCCGGTGCGTGGCGCGTGTCAAAGGCGGCGACCCATTGTTGTTCGGTCGCGGCGGCGAGGAAGCAGAGTTCCTGCG
CGGGCACGGCATCGAAGTGGAAATTGTCAACGGCATCAGCAGTGGCATGGCTGCTGCGCAGGCGCTTGGCATTGCGCTGA
CCCATCGTCAGCATTGCCACGGTGTGACCTTCGTTACCGCGCATACGCGTGACGATGGCGATCCCGATTGGGCCGCATTG
GTCGGCAGCCGGACGACGCTCGTCATCTACATGGGGATGAGCCGTCTTGCCGCCATTCGCGACGCCTTGCTGCAAGCGGG
TATGCGGTCGGAAATGCCGGTTGCGGTGGTGATGCATGCTAGCCGTGGGGATGAGAAGCGTTGGGTTGGCAGCCTGGCCA
CATTGACCGAGGCTGCGGATGCCGGGCTTGCCAGCCCGGCGGTCATTCTGGTTGGGGAAGTCGTTGATATCCCCAACCCT
GCTTCATTGACGGACCAGCACGCCTTCGGCGACCTTGCCGCTGGGCTGCCGTCCAAACAGCCCGAGCACGACCTGCAGCG
AGAACGACAGCGCGCCCACCAGGAAGACCACATCACCGAAGGTACGCATCCAGCGTAG

Upstream 100 bases:

>100_bases
AGGAAATCAAGCGACTGCCGATGGACAAGCCGATCGGCAAGTACAACGTATTCAACAAGACTACGCGCTCGTCTGGCACG
AGCCATTGAGGAGTACCAAC

Downstream 100 bases:

>100_bases
CGTTTGCAGGAGTGGCTGCTGCATGAAGGCCTCGCTGCGTGCATACCACAGGCCTTCACTTGCGCTGGCGATGAACTGGA
TGATACCCACCGGCAACAGG

Product: Uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKRGGRCSVSQSSIHEQALEHAQ
AGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGISSGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAAL
VGSRTTLVIYMGMSRLAAIRDALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNP
ASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA

Sequences:

>Translated_285_residues
MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKRGGRCSVSQSSIHEQALEHAQ
AGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGISSGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAAL
VGSRTTLVIYMGMSRLAAIRDALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNP
ASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA
>Mature_285_residues
MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKRGGRCSVSQSSIHEQALEHAQ
AGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGISSGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAAL
VGSRTTLVIYMGMSRLAAIRDALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNP
ASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=243, Percent_Identity=38.2716049382716, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=33.1932773109244, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 30229; Mature: 30229

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEHHHCCCCEEEECCCCCEEEEECCC
GGRCSVSQSSIHEQALEHAQAGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGIS
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHHHCCCCEEEEECCCH
SGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAALVGSRTTLVIYMGMSRLAAIR
HHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEECHHHHHHHH
DALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNP
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCC
ASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEHHHCCCCEEEECCCCCEEEEECCC
GGRCSVSQSSIHEQALEHAQAGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGIS
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHHHCCCCEEEEECCCH
SGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAALVGSRTTLVIYMGMSRLAAIR
HHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEECHHHHHHHH
DALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNP
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCC
ASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]