BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is paaF [H]

Identifier: 94312093

GI number: 94312093

Start: 3433325

End: 3434101

Strand: Reverse

Name: paaF [H]

Synonym: Rmet_3162

Alternate gene names: 94312093

Gene position: 3434101-3433325 (Counterclockwise)

Preceding gene: 94312097

Following gene: 335055651

Centisome position: 87.42

GC content: 62.81

Gene sequence:

>777_bases
ATGTCGTACGAGAACATCCTGGTGGAGACGCGAGGTCGCGTTGGTCTGGTGACGCTGAACCGGCCCAAAGCGCTCAATGC
GCTCAATGACGCGCTGATGGACGAACTGGGCGCGGCGCTGACGGCATTCGACCAGGACGAGGCAATCGGCTGCATCGTCA
TCACGGGGAGCGAGCGTGCCTTCGCCGCAGGGGCCGATATCAGCATGATGGCGAAGTACTCCTATATGGATGTCTACAAG
GGCGACTACATCACCCGCAACTGGGAGACAATCCGCCGCATCCGCAAACCGGTGATCGCCGCCGTGGCCGGCTATGCGCT
GGGCGGTGGTTGCGAACTGGCGATGATGTGCGACATTGTGATTGCCGCCGACAGCGCGAAGTTCGGCCAGCCGGAAGTGA
AGCTCGGCACGATGCCTGGCGCCGGCGGCACGCAGCGGCTGCCGCGTGCCGTCTCCAAGGCCAAGGCGATGGACCTGTGC
CTGACCGCGCGCATGATGGATGCCACCGAGGCCGAGCGCGCCGGGCTGGTTTCCCGGATCGTCCCGGCAGACAAACTGGT
AGACGAAGCCGTGGCCGCGGCCGAGACTATCGCCGGTTACTCGCTGCCGGTTGTCATGATGATCAAGGAGTCGGTGAACG
CTGCCTATGAGACGACTCTGAATGAAGGGGTCCACTTCGAGCGCCGACTGTTCCACTCGACCTTCGCCACCGAAGACCAG
AAGGAGGGTATGGCAGCGTTTGTGGAGAAGCGTGCACCCAATTTCCAGAATCGGTGA

Upstream 100 bases:

>100_bases
GCAAGAGGCTGGTGCGCTTGACCTGCTGGGCGCAAGCACGCCAGTATCGTCCCCGCTGACACTCGACAAATAGAACCCAA
CCGCATTGCAAGGAGCAGAC

Downstream 100 bases:

>100_bases
CCCTGGACTAGGGAATACCCGAGGATAATCAAGGGTTATTAAATGGGCCGGAAAGTTATTTCCGGCCCATTTTTTCTTTA
TGATCAATGACTTGCCGAGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALTAFDQDEAIGCIVITGSERAFAAGADISMMAKYSYMDVYK
GDYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIVIAADSAKFGQPEVKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMDATEAERAGLVSRIVPADKLVDEAVAAAETIAGYSLPVVMMIKESVNAAYETTLNEGVHFERRLFHSTFATEDQ
KEGMAAFVEKRAPNFQNR

Sequences:

>Translated_258_residues
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALTAFDQDEAIGCIVITGSERAFAAGADISMMAKYSYMDVYK
GDYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIVIAADSAKFGQPEVKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMDATEAERAGLVSRIVPADKLVDEAVAAAETIAGYSLPVVMMIKESVNAAYETTLNEGVHFERRLFHSTFATEDQ
KEGMAAFVEKRAPNFQNR
>Mature_257_residues
SYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALTAFDQDEAIGCIVITGSERAFAAGADISMMAKYSYMDVYKG
DYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIVIAADSAKFGQPEVKLGTMPGAGGTQRLPRAVSKAKAMDLCL
TARMMDATEAERAGLVSRIVPADKLVDEAVAAAETIAGYSLPVVMMIKESVNAAYETTLNEGVHFERRLFHSTFATEDQK
EGMAAFVEKRAPNFQNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=56.5384615384615, Blast_Score=303, Evalue=7e-83,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=38.1322957198444, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=37.037037037037, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=33.4586466165414, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=28.6885245901639, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=36.1581920903955, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.9672131147541, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=32.1052631578947, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=32.1052631578947, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=23.7547892720307, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=23.7547892720307, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=23.8461538461538, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=23.8461538461538, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45643119, Length=190, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=190, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=23.9316239316239, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=23.9316239316239, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=25.5924170616114, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=25.5924170616114, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=42.2680412371134, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=23.1660231660232, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=23.1660231660232, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.2687747035573, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=35.2272727272727, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=35.1145038167939, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=30.3846153846154, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=31.8407960199005, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=32.2222222222222, Blast_Score=98, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=56.5737051792829, Blast_Score=295, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=56.198347107438, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=34.2629482071713, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=30, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=203, Percent_Identity=35.4679802955665, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=201, Percent_Identity=37.3134328358209, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=201, Percent_Identity=37.3134328358209, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=201, Percent_Identity=37.3134328358209, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=273, Percent_Identity=27.8388278388278, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=34.0782122905028, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=30.5699481865285, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=30.5699481865285, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=262, Percent_Identity=25.5725190839695, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=28.3505154639175, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=57.9150579150579, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=57.9150579150579, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=34.5864661654135, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=30.3501945525292, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=28.0632411067194, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=29.5774647887324, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=32.1285140562249, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=30.4812834224599, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=190, Percent_Identity=27.8947368421053, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571730, Length=190, Percent_Identity=27.8947368421053, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27902; Mature: 27771

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALTAFDQDEAIGCIVITGSERA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADISMMAKYSYMDVYKGDYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIV
EECCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IAADSAKFGQPEVKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMDATEAERAGLVSRI
EEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADKLVDEAVAAAETIAGYSLPVVMMIKESVNAAYETTLNEGVHFERRLFHSTFATEDQ
CCHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRAPNFQNR
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALTAFDQDEAIGCIVITGSERA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADISMMAKYSYMDVYKGDYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIV
EECCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IAADSAKFGQPEVKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMDATEAERAGLVSRI
EEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADKLVDEAVAAAETIAGYSLPVVMMIKESVNAAYETTLNEGVHFERRLFHSTFATEDQ
CCHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRAPNFQNR
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]