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The map label for this gene is mpl [H]

Identifier: 94311991

GI number: 94311991

Start: 3317073

End: 3318506

Strand: Reverse

Name: mpl [H]

Synonym: Rmet_3060

Alternate gene names: 94311991

Gene position: 3318506-3317073 (Counterclockwise)

Preceding gene: 94312002

Following gene: 94311990

Centisome position: 84.48

GC content: 66.53

Gene sequence:

>1434_bases
TTGCCGCGCCACGCATCGCGCCCGGCGCGGCCCCACGCATTTTCCCGAGCTTCCATGCATATTCATATCCTTGGCATCTG
CGGTACGTTCATGGGCGGCCTGGCTGTCCTGGCCCGCCAGGCGGGGCACCGCGTGACTGGCTGCGACGCCAATGTCTATC
CGCCCATGAGCACCCAGCTCGAAGCCCAGGGCATCGACCTGATCGAGGGGTTCGACCCCGACCAGCTTTCGCTGAAGCCG
GACCTGTTCGTGATCGGCAATGTGGTCTCGCGCGGCAATCCGCTGATGGAAGCGATCCTGAACCGCAACCTGCCGTACGT
TTCGGGGCCGCAATGGCTCGGCGAGCACGTGCTCAATGGCAAGTGGACGCTGGCCGTGGCTGGCACGCATGGCAAGACCA
CGACCACGTCGATGCTGGCGTGGATTCTGGAAGACGCCGGCTACAACCCCGGCTTTCTGGTGGGCGGGGTGCCGCAGAAC
TTCGGCATTTCGGCGCGCCTGACGGAATCGGACTTCTTCGTTATCGAGGCCGACGAGTACGACACCGCGTTCTTCGACAA
GCGCAGCAAGTTCGTCCACTACCGTCCGCGTACGGCGATTCTCAACAACCTTGAGTACGATCACGCCGACATCTTCCCGG
ATCTGACCGCGATCGAGACCCAATTCCATCATCTGGTGCGTACCGTGCCGGGCGAGGGGCGGCTGGTCGTCAACGGCCAG
GAGCCCGCACTGGCACGCGTGCTGGAGCGCGGCTGCTGGAGCGAGGTGGAGCAGTTCGGCGTGGGCGACTGGAGCGAGAC
CGATCCGAAGACCGCCGCGCCAGAGGGCAAGGATGCCTTCGACATCTGGTTCCGCGAGGCGCTGCAGGGCACGCTGGTCT
GGGACCTTCAGGGTACCCACAACCGCCTGAACGCCATTGCCGCGATTGCCGCCGCGCGCCACGTGGGCGTGCCGCCGGCG
CAGGCGATCGAGTCGCTTGGCCGTTTCTCCAACGTCAAGCGCCGGATGGAAGTACGCGGCGTGGCGGGTGGTGTGACGGT
CTACGACGACTTCGCGCACCATCCCACGGCCATCCAGACCACGGTCGACGGGCTGCGCCGCCGGGTGGGCGACGCACGCA
TCCTCGCCGTATTGGAGCCGCGTTCGAACACCATGAAGCTTGGCGTGATGGCTGCCCAATTGCCGGCCAGCCTGCGCGAG
GCTGACCTTGTGTTCGGATATGGCGCGCCCTCTGGCAAGGACGCGCTGGGGTGGAACCTGGCCGAAGCGCTGGCCCCCCT
CGGCGAGAAGGCCGCGGCGTTCCAGGACCTCGGCACGCTGGTGGAAGCCGTGCGCGCTGCCGCCCAGCCCGGCGACCAGG
TGCTCGTGATGAGCAACGGCGGCTTCGGCGGGGTACATCAGAAACTGCTCGACGCGCTCGGCGCGAAGTCCTGA

Upstream 100 bases:

>100_bases
GATGGGGTCGGAAGGCATACGTCGGGACATGCTGCGATGATACCCGCGCGGACGCAGTCGGGGCCTTCGACCCACGCGGT
ACAATCGGGACCTTGTTCAG

Downstream 100 bases:

>100_bases
CCGGGAATCCACATGCTGCTGTACCTGCACGGTTTCCGTTCCTCGCCGCAGTCGTTCAAGTCGCGGGTCGTGCAGGACCG
GATGCGCGCCTGGGGCGTGG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPRHASRPARPHAFSRASMHIHILGICGTFMGGLAVLARQAGHRVTGCDANVYPPMSTQLEAQGIDLIEGFDPDQLSLKP
DLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLNGKWTLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQN
FGISARLTESDFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLTAIETQFHHLVRTVPGEGRLVVNGQ
EPALARVLERGCWSEVEQFGVGDWSETDPKTAAPEGKDAFDIWFREALQGTLVWDLQGTHNRLNAIAAIAAARHVGVPPA
QAIESLGRFSNVKRRMEVRGVAGGVTVYDDFAHHPTAIQTTVDGLRRRVGDARILAVLEPRSNTMKLGVMAAQLPASLRE
ADLVFGYGAPSGKDALGWNLAEALAPLGEKAAAFQDLGTLVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGAKS

Sequences:

>Translated_477_residues
MPRHASRPARPHAFSRASMHIHILGICGTFMGGLAVLARQAGHRVTGCDANVYPPMSTQLEAQGIDLIEGFDPDQLSLKP
DLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLNGKWTLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQN
FGISARLTESDFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLTAIETQFHHLVRTVPGEGRLVVNGQ
EPALARVLERGCWSEVEQFGVGDWSETDPKTAAPEGKDAFDIWFREALQGTLVWDLQGTHNRLNAIAAIAAARHVGVPPA
QAIESLGRFSNVKRRMEVRGVAGGVTVYDDFAHHPTAIQTTVDGLRRRVGDARILAVLEPRSNTMKLGVMAAQLPASLRE
ADLVFGYGAPSGKDALGWNLAEALAPLGEKAAAFQDLGTLVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGAKS
>Mature_476_residues
PRHASRPARPHAFSRASMHIHILGICGTFMGGLAVLARQAGHRVTGCDANVYPPMSTQLEAQGIDLIEGFDPDQLSLKPD
LFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLNGKWTLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNF
GISARLTESDFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLTAIETQFHHLVRTVPGEGRLVVNGQE
PALARVLERGCWSEVEQFGVGDWSETDPKTAAPEGKDAFDIWFREALQGTLVWDLQGTHNRLNAIAAIAAARHVGVPPAQ
AIESLGRFSNVKRRMEVRGVAGGVTVYDDFAHHPTAIQTTVDGLRRRVGDARILAVLEPRSNTMKLGVMAAQLPASLREA
DLVFGYGAPSGKDALGWNLAEALAPLGEKAAAFQDLGTLVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGAKS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=461, Percent_Identity=59.4360086767896, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1786279, Length=383, Percent_Identity=28.9817232375979, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51530; Mature: 51399

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRHASRPARPHAFSRASMHIHILGICGTFMGGLAVLARQAGHRVTGCDANVYPPMSTQL
CCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
EAQGIDLIEGFDPDQLSLKPDLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLNG
CCCCCHHCCCCCCCCCCCCCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCC
KWTLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNFGISARLTESDFFVIEADEY
EEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCC
DTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLTAIETQFHHLVRTVPGEGRLVVNGQ
CHHHHHCCCCEEEECCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCC
EPALARVLERGCWSEVEQFGVGDWSETDPKTAAPEGKDAFDIWFREALQGTLVWDLQGTH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCH
NRLNAIAAIAAARHVGVPPAQAIESLGRFSNVKRRMEVRGVAGGVTVYDDFAHHPTAIQT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
TVDGLRRRVGDARILAVLEPRSNTMKLGVMAAQLPASLREADLVFGYGAPSGKDALGWNL
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEHHHCCHHHHCCCEEEECCCCCCCCCCCCCH
AEALAPLGEKAAAFQDLGTLVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGAKS
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRHASRPARPHAFSRASMHIHILGICGTFMGGLAVLARQAGHRVTGCDANVYPPMSTQL
CCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
EAQGIDLIEGFDPDQLSLKPDLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLNG
CCCCCHHCCCCCCCCCCCCCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCC
KWTLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNFGISARLTESDFFVIEADEY
EEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCC
DTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLTAIETQFHHLVRTVPGEGRLVVNGQ
CHHHHHCCCCEEEECCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCC
EPALARVLERGCWSEVEQFGVGDWSETDPKTAAPEGKDAFDIWFREALQGTLVWDLQGTH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCH
NRLNAIAAIAAARHVGVPPAQAIESLGRFSNVKRRMEVRGVAGGVTVYDDFAHHPTAIQT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
TVDGLRRRVGDARILAVLEPRSNTMKLGVMAAQLPASLREADLVFGYGAPSGKDALGWNL
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEHHHCCHHHHCCCEEEECCCCCCCCCCCCCH
AEALAPLGEKAAAFQDLGTLVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGAKS
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]