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The map label for this gene is phcA [H]

Identifier: 94311909

GI number: 94311909

Start: 3235793

End: 3236794

Strand: Reverse

Name: phcA [H]

Synonym: Rmet_2977

Alternate gene names: 94311909

Gene position: 3236794-3235793 (Counterclockwise)

Preceding gene: 335055640

Following gene: 94311907

Centisome position: 82.4

GC content: 60.58

Gene sequence:

>1002_bases
ATGGTGAACGTGGATACCAAGCTGCTCGTGATCTTCACCGAGTTGCTCAGCAAGCGTAACGCCACTTATGTGGCGGAAAA
GATGCACATGACCGCGCCGGCCGTTTCTCACTCGCTCGGCCGACTGCGCGAAATCTTCGACGATCCCCTTTTCATTCGGG
TGCCTCATGGTCTCACGCCAACGCCGCGCGCGCTGGAACTGGGCCCGAAGATCCGGGACATGCTCGACCTCTGGTCGTCG
ATCAACGAGGGGGATGCCGATAATTTCGATCCGGCCATCGCCACCGGCACGCTGAATATCGGTTTTGCCGCCGAACTTGG
TGACACGGTTTTCAACCGCTTTGTCCTGCGCATCAAGCAACTGGCTCCGGACCTGCATATCCGCCTGGTCGAATCGCATT
CGTGGGAAGCCGACGTCGCGTCGATGCGTGCAAACGAACTCGATCTGGCCTTTTCGCCGTTCCCGACCCGCCATCCCGAA
ATCGTTGAGGAAGTGGTGACGTCGCTGAACCTGTGGGTTTGCGCACGCAAGAATCATCCGCTCCTGAAGAACGATTGCAC
GCTCGAGCAATACCTCGACTGCAGCCATATCTTTATGGCGCATTCCGGTGGCGCGGGCCGTCCGGCACCGGCACTGATTC
CGTTGGACTACGCGCTGCAGCAGCGGGGCCTGAAGCGGAATGCCACGCTGACCGTTCATTCGTGGCGCGCCCAGGCCGAA
CTGGCTTCCCAGACCGACATGATCTTCACGGTCAACGCGCTGACCAAGGACATGGCCTGTGAAACGTATGGCCTGAAGGC
CTACCCGCTGCCGGCGGAGCTCAATACGATGCTGGGCCTGAATATGTTCTGGCACCGCAGCCGCAATACCCATCCGATGC
TGGTTTGGGCACGTGGTCTCTTCCGTCAGGTGGTTGGCGAGTTTCTGGGCCTGCCTGCGGCAGCCGCTGCCGTGCCGCGT
AGCCTGCGCGTCGTGGCCGAGCAGGATCTGACGCAGGAATAA

Upstream 100 bases:

>100_bases
ATTTTCTCTTACACTCGGAAGGATCTATTAATGTCTGATGTGCAGCCAGTGAAAGTGGTGGGGCCATCGAAGCGTAAGAC
ATAACAAACCGGGCCTGATC

Downstream 100 bases:

>100_bases
CGCTGTGTCGACCCAATAAAAAACCGGCGCCTTGGCGCCGGTTTTTTATTGCCTGCTGCTGGGCGGGTACCGCAGCGGGG
TTATCGTCTCGGTCTGTGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Virulence genes transcriptional regulator phcA [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQLAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPE
IVEEVVTSLNLWVCARKNHPLLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAE
LASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGLFRQVVGEFLGLPAAAAAVPR
SLRVVAEQDLTQE

Sequences:

>Translated_333_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQLAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPE
IVEEVVTSLNLWVCARKNHPLLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAE
LASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGLFRQVVGEFLGLPAAAAAVPR
SLRVVAEQDLTQE
>Mature_333_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQLAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPE
IVEEVVTSLNLWVCARKNHPLLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAE
LASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGLFRQVVGEFLGLPAAAAAVPR
SLRVVAEQDLTQE

Specific function: Regulates the transcription of one or more of the genes involved in virulence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=289, Percent_Identity=25.2595155709343, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790147, Length=316, Percent_Identity=26.2658227848101, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081766, Length=296, Percent_Identity=22.6351351351351, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37182; Mature: 37182

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPRALELGPKIRDMLDLWSSINEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQ
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
LAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPEIVEEVVTSLNLWVCARKNHP
HCCCEEEEEEECCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCEEEEEECCCCC
LLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAE
CCCCCCCHHHHCCHHHEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCEEEEECCHHHHH
LASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGL
HHCCCCEEEEEHHHHHHHHHHHCCCEECCCCHHHHHHHCCHHHEECCCCCCCEEHHHHHH
FRQVVGEFLGLPAAAAAVPRSLRVVAEQDLTQE
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPRALELGPKIRDMLDLWSSINEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQ
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
LAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPEIVEEVVTSLNLWVCARKNHP
HCCCEEEEEEECCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCEEEEEECCCCC
LLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAE
CCCCCCCHHHHCCHHHEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCEEEEECCHHHHH
LASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGL
HHCCCCEEEEEHHHHHHHHHHHCCCEECCCCHHHHHHHCCHHHEECCCCCCCEEHHHHHH
FRQVVGEFLGLPAAAAAVPRSLRVVAEQDLTQE
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366033; 10913109; 11823852 [H]