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The map label for this gene is phcR [H]

Identifier: 94311882

GI number: 94311882

Start: 3207687

End: 3208823

Strand: Reverse

Name: phcR [H]

Synonym: Rmet_2950

Alternate gene names: 94311882

Gene position: 3208823-3207687 (Counterclockwise)

Preceding gene: 94311883

Following gene: 94311881

Centisome position: 81.69

GC content: 63.15

Gene sequence:

>1137_bases
GTGACGACCGATACGAGCCAGCCGCGCGCGACCATTCTCTACGTCGATGACGAGGAGATGGCGTGCAAGTATTTCGCGCG
CGCGGCCGGCAGCGAACACGAAGTACTTTCCGCCACCGGCGCGGATGAGGCGATAGCCATCCTGCAGGAGCATCAAGCGA
AGATCTCGGTGCTGGTCACCGACTATCGGATGCCGGGCCGCGATGGTGGCGACCTGCTGCGACAGGTGGCGCTGGAGTAT
CCGCAGATCGTGCGGATACTGGTCACGGCCTATGCCGACAAGGACATGCTGCTCGAGACCGTCAATAGCGGCGAAGTCTT
CAAGATTCTCGAGAAGCCGATCACCGTGACCGATGTGCGCGAGGTGCTGCGCCTGGCCGGAGAGCGTTATCGCGAACGCG
CGGTGCGTCAGCAAAGGCTGATGGCGATCGACGAGACGCTGGCCTTTCTGGCCCATGAACTGAATACGCCGCTGGCGGCG
ATTGCCAACTTCGCACGCGGGATCGAGGCGCGCGTGGCTGGCGAGTACAACCAGCAGCGTAATGCCGAGATCGGCCGCGC
GGCCGGTGCCATGCATGACAACGCCCAGTACTGCCTTGCAGTGCTGTCGTCGTTCCTGCAGTCCGTGCGCAGCAGCGCGG
GCGGTGCCAAGCCGAAGCCGGGTCCGGGCAATGACGTCAGTGCGGGGGCATTGGTGGCATCCCTGCTGGATAGCTATCCG
TTTGCCGATAACCAACGTAACTGGGTGCAAGTCGAGTTGCACGACGATTTTCCCGTGCCGACGCTGCCGAACTGCGTGGC
GCTGGTGCTGTCGTCGGTGATGAGCAATGCGCTGCGCGCGCTGGCCGATGTGGACAACCCGTCGCTGCGCTTTGTCGTGG
TGGCTGGAGATCGGCCGGAAATCCGCATCGCGGACAACGGGCCCGGCATTCCGCCGGAGGTGATGGATCGTCTGCTCGTC
GACCCCGTCACCACGCACGCCTCGGCGGGCGGCAGCGGGATGGGCATGATCTTCTGTAACCGCGTCATGCAATCTTTTGG
TGGCGGCATCCGGATCGCTTCGGCTTCGGGCGCAGGCACCACCGTAACCCTCGATTTCCCCAATATGAAGAATCGTATGA
ACAGGAGTGATCGATGA

Upstream 100 bases:

>100_bases
CTACGCCGTGATCGAACGTCATGGCGGCGTGCTGTATGCCGAGAGCGAAGTGGGCCAGTGGGCCGCCTTCAGCTTCGACC
TGCCGCGTGCGGAGTAAGCC

Downstream 100 bases:

>100_bases
GCGAACCGAATACCACGCAGGCACCCCCGGCAATTCTGTTCGTCGATGACGAGGCCACGGCCGTCAAGTATTTCCAGCGC
GCCATTGGCCAGCTTGCCCC

Product: signal transduction histidine kinase containing a receiver domain (hybrid)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVTDYRMPGRDGGDLLRQVALEY
PQIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVREVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAA
IANFARGIEARVAGEYNQQRNAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYP
FADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPEIRIADNGPGIPPEVMDRLLV
DPVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNMKNRMNRSDR

Sequences:

>Translated_378_residues
MTTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVTDYRMPGRDGGDLLRQVALEY
PQIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVREVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAA
IANFARGIEARVAGEYNQQRNAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYP
FADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPEIRIADNGPGIPPEVMDRLLV
DPVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNMKNRMNRSDR
>Mature_377_residues
TTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVTDYRMPGRDGGDLLRQVALEYP
QIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVREVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAAI
ANFARGIEARVAGEYNQQRNAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYPF
ADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPEIRIADNGPGIPPEVMDRLLVD
PVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNMKNRMNRSDR

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=24.5901639344262, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 40872; Mature: 40741

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVT
CCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCEEEEEEE
DYRMPGRDGGDLLRQVALEYPQIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVR
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCHHHHH
EVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAAIANFARGIEARVAGEYNQQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHCCCCCHHH
NAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYP
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPE
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
IRIADNGPGIPPEVMDRLLVDPVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGT
EEECCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
TVTLDFPNMKNRMNRSDR
EEEEECCCHHHHCCCCCC
>Mature Secondary Structure 
TTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVT
CCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCEEEEEEE
DYRMPGRDGGDLLRQVALEYPQIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVR
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCHHHHH
EVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAAIANFARGIEARVAGEYNQQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHCCCCCHHH
NAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYP
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPE
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
IRIADNGPGIPPEVMDRLLVDPVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGT
EEECCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
TVTLDFPNMKNRMNRSDR
EEEEECCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]