Click here to switch to the map view.

The map label for this gene is phcS [H]

Identifier: 94311883

GI number: 94311883

Start: 3208827

End: 3210215

Strand: Reverse

Name: phcS [H]

Synonym: Rmet_2951

Alternate gene names: 94311883

Gene position: 3210215-3208827 (Counterclockwise)

Preceding gene: 94311884

Following gene: 94311882

Centisome position: 81.72

GC content: 63.5

Gene sequence:

>1389_bases
ATGTCTTCGCCGAAGCCGCTATCCGTTGAGGATTCGGCCGCCCAACCGGCGAAGGCGATGAGTTCGAACAAGCTATACGC
CGGATACCAGTCCGAACTGGCCGATTTCCGCCTGGCATTCAGCCGGGGCGGCGCGTACACGGCTATCGTGCTGGTGCTGC
TCGGGGTTGGGCTCGACTACGGCCAGTATCCGCAGTGGCAAGTGCCATTCGCTGCGGCGCGCATCGTCGTTTCGCTGCTG
ATCGCAGGCGTGGTGGTCGCGCTCTACAGCCAGGCTGGCCGCCGCTTCGCGCCGTGGCTCACCATGACGTGGCTGCTGCT
GCCGCAGATCATGATCGCCTGGATGATCTCGCAGACCGAAGGCGTCGAATCGCCGTACTACGTCGGGCTGAATCTCGCGA
TCTTCGCCTCGGGCATCGCCTTGCCGTTTGGGCTGTGGCAAAACCTGGTGTTCGGCGTCCTGTCGTATGTGCTTTATGCC
GCCGCGTGCCTGCTGCACCCGGGCGGCATCGAGCCCGTGGGGACGTTCATCGTCAATTCGCTGTTCCTGCTGTTTGCCGC
GGCAGCGAGTGGCGTGTACACGTTCTTCAATGAACGCGCGCGCTTCATGCTGTTCCGCTTGAAGGCCGAGGTGGCGGACA
AGAACGCGGAGCTCGAGGTCATCAATCGCAAGCTGGTCGACATCAAGGGCCAGATGCTTCAGCAGGAGAAGATGGCGGCC
ATCGGCACGTTGGCGGCGGGCCTGCTGCACGAGGTGAACAATCCGGTCAACTTCTGCCTGATGGCGATCGAAGTGGCCAT
GGAGGAACCGGCCGCGAAGTCCGAACCGGGCATCCAGGAGTGTCTGGTCGATGCCAAACAGGGTATGCAGCGCATCCAGC
ATATCGTCTCCGACCTCAAGACATTCGCCTACCGCAAGCCGGGCGCCGAGGTGGAAGGCACCCCGTTCCTGTTCGAGAAG
GCACTTGATTCGTCGCAACGGCTGACTGCGCACGAACTGCGTGGGGTCAAGATGACGCGCGAGATGCCAGACGACACCCT
CGTCCTTGGCGACGAGGCCGCGATCATCGGCGTGCTGATCAACCTGTTCTCGAACGCCGCGCTGGCGATGCGCAAGGCGG
GTACGCAGAATCCGGCGATCCATACGACGGTGCGGTGGGAGGACAAACGCCTGCGCGTGACCGTGCGCGACAACGGTCCT
GGCATCGCGCAGGAGCATCTCGCCCGCGTGTTCGAGCCGTTCTTCACCACGCGGGAAGTGGGCCAGGGCCTGGGTCTTGG
CCTGTCGATCAGCTACGCCGTGATCGAACGTCATGGCGGCGTGCTGTATGCCGAGAGCGAAGTGGGCCAGTGGGCCGCCT
TCAGCTTCGACCTGCCGCGTGCGGAGTAA

Upstream 100 bases:

>100_bases
CTGGAGTGGTACCTGATCTATCGCGACGAGGCCGGTATGGCCTCGCTGTTGCCGGAGAACTCGCATGCCCACCGACTCTA
TGTCGATGAAACCGGCGTCA

Downstream 100 bases:

>100_bases
GCCGTGACGACCGATACGAGCCAGCCGCGCGCGACCATTCTCTACGTCGATGACGAGGAGATGGCGTGCAAGTATTTCGC
GCGCGCGGCCGGCAGCGAAC

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSSPKPLSVEDSAAQPAKAMSSNKLYAGYQSELADFRLAFSRGGAYTAIVLVLLGVGLDYGQYPQWQVPFAAARIVVSLL
IAGVVVALYSQAGRRFAPWLTMTWLLLPQIMIAWMISQTEGVESPYYVGLNLAIFASGIALPFGLWQNLVFGVLSYVLYA
AACLLHPGGIEPVGTFIVNSLFLLFAAAASGVYTFFNERARFMLFRLKAEVADKNAELEVINRKLVDIKGQMLQQEKMAA
IGTLAAGLLHEVNNPVNFCLMAIEVAMEEPAAKSEPGIQECLVDAKQGMQRIQHIVSDLKTFAYRKPGAEVEGTPFLFEK
ALDSSQRLTAHELRGVKMTREMPDDTLVLGDEAAIIGVLINLFSNAALAMRKAGTQNPAIHTTVRWEDKRLRVTVRDNGP
GIAQEHLARVFEPFFTTREVGQGLGLGLSISYAVIERHGGVLYAESEVGQWAAFSFDLPRAE

Sequences:

>Translated_462_residues
MSSPKPLSVEDSAAQPAKAMSSNKLYAGYQSELADFRLAFSRGGAYTAIVLVLLGVGLDYGQYPQWQVPFAAARIVVSLL
IAGVVVALYSQAGRRFAPWLTMTWLLLPQIMIAWMISQTEGVESPYYVGLNLAIFASGIALPFGLWQNLVFGVLSYVLYA
AACLLHPGGIEPVGTFIVNSLFLLFAAAASGVYTFFNERARFMLFRLKAEVADKNAELEVINRKLVDIKGQMLQQEKMAA
IGTLAAGLLHEVNNPVNFCLMAIEVAMEEPAAKSEPGIQECLVDAKQGMQRIQHIVSDLKTFAYRKPGAEVEGTPFLFEK
ALDSSQRLTAHELRGVKMTREMPDDTLVLGDEAAIIGVLINLFSNAALAMRKAGTQNPAIHTTVRWEDKRLRVTVRDNGP
GIAQEHLARVFEPFFTTREVGQGLGLGLSISYAVIERHGGVLYAESEVGQWAAFSFDLPRAE
>Mature_461_residues
SSPKPLSVEDSAAQPAKAMSSNKLYAGYQSELADFRLAFSRGGAYTAIVLVLLGVGLDYGQYPQWQVPFAAARIVVSLLI
AGVVVALYSQAGRRFAPWLTMTWLLLPQIMIAWMISQTEGVESPYYVGLNLAIFASGIALPFGLWQNLVFGVLSYVLYAA
ACLLHPGGIEPVGTFIVNSLFLLFAAAASGVYTFFNERARFMLFRLKAEVADKNAELEVINRKLVDIKGQMLQQEKMAAI
GTLAAGLLHEVNNPVNFCLMAIEVAMEEPAAKSEPGIQECLVDAKQGMQRIQHIVSDLKTFAYRKPGAEVEGTPFLFEKA
LDSSQRLTAHELRGVKMTREMPDDTLVLGDEAAIIGVLINLFSNAALAMRKAGTQNPAIHTTVRWEDKRLRVTVRDNGPG
IAQEHLARVFEPFFTTREVGQGLGLGLSISYAVIERHGGVLYAESEVGQWAAFSFDLPRAE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=294, Percent_Identity=29.5918367346939, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=28.0851063829787, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=28.8617886178862, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=297, Percent_Identity=26.5993265993266, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=241, Percent_Identity=24.0663900414938, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50524; Mature: 50393

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SSPKPLSVEDSAAQPAKAMSSNKLYAGYQSELADFRLAFSRGGAYTAIVLVLLGVGLDY
CCCCCCCCCCCCCCCHHHHCCCCEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
GQYPQWQVPFAAARIVVSLLIAGVVVALYSQAGRRFAPWLTMTWLLLPQIMIAWMISQTE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GVESPYYVGLNLAIFASGIALPFGLWQNLVFGVLSYVLYAAACLLHPGGIEPVGTFIVNS
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LFLLFAAAASGVYTFFNERARFMLFRLKAEVADKNAELEVINRKLVDIKGQMLQQEKMAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGTLAAGLLHEVNNPVNFCLMAIEVAMEEPAAKSEPGIQECLVDAKQGMQRIQHIVSDLK
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TFAYRKPGAEVEGTPFLFEKALDSSQRLTAHELRGVKMTREMPDDTLVLGDEAAIIGVLI
HHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCHHHHCCCCCEEEECCCHHHHHHHH
NLFSNAALAMRKAGTQNPAIHTTVRWEDKRLRVTVRDNGPGIAQEHLARVFEPFFTTREV
HHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GQGLGLGLSISYAVIERHGGVLYAESEVGQWAAFSFDLPRAE
HCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]