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The map label for this gene is glcE [H]

Identifier: 94311860

GI number: 94311860

Start: 3184448

End: 3185551

Strand: Direct

Name: glcE [H]

Synonym: Rmet_2928

Alternate gene names: 94311860

Gene position: 3184448-3185551 (Clockwise)

Preceding gene: 94311859

Following gene: 94311861

Centisome position: 81.07

GC content: 68.48

Gene sequence:

>1104_bases
ATGCAAGCCACTCTCGACGCCTTTCGCGATGCGATCCGCCAAGCCGGCGACACCCGCACACCGCTGCGCCTGCGCGGTGG
CGGCAGCAAGGACTTCTATGGCCAGGCGCTGGCCGGCACCGTGCTCGATACGCGGGCCTATCGCGGCATCGTCGAATACG
ATCCCGCCGAACTCGTGATCACGGCACGCTGCGGCACGCCGCTGGCCGAGATCGAGGACGCGGTGGCGCAGAAGCGCCAG
ATGCTGGCCTTCGAGCCGCCGCATTTCGCGCTGCGCGGCCAGGCCAGCACGGCCACGATCGGCGGCGCGGTGGCGGCCGG
GTTGTCCGGTCCTCGCCGTCAGTCCGCGGGAGCGCTGCGCGACTTCGTGCTGGGCGCGCAATTGATGGACGGCCACGGCG
AGATCATGAACTTCGGTGGCCAGGTGATGAAGAACGTGGCCGGCTATGACGTGTCGCGCCTGCTGGCCGGCTCGCTCGGC
ACGCTCGGCCTGATTCTCGAGGTCTCGCTGAAGGTGCTGCCCGTGCCATTCGATGACGCCACGTTGCGCTTCGCGATGGA
CCAGGCCGAGGCGATCGACCAGCTCAACAAATGGGGCGGCCAGCCGTTGCCGATCGCGGCGTCGGCGTGGCACGACGGAT
TCCTGCATGTGCGACTCAGCGGCGCGACCGCCGCAGTTTGCGCCGCGCGTGACAACCTGGGCGGCGAGGTCGTCGACGCC
AGCCACGCCCAGCAGTTGTGGATGGTCTTGCGCGAACAGACGCACGCGTTCTTCTCCCCAGCAAACGATACGGGCCGCGC
CCTGTGGCGCATCGCCGTACCGACGGTCTGCAAGCCGCTGGACCTGCCCGGCGCTCAGCTCGTGGAATGGGGTGGCGGCC
AGCGCTGGTGGATCGCTCCGGATGACAGCGCCGCATCGGCGGATCGGGTACGCGCCGCAGCGCAGGCAGCCGGTGGTCAC
GCCACGCTGTTCCGCAATGGCGACAAGTCCGTTGGCGTGTTCACGCCGCTGTCATCGCCGCTGGCCGCGATCCACAAGCG
GCTCAAGGACACCTTCGACCCTGCGGGCATTTTCAATCCGCAGCGCATGTACAACGGACTCTGA

Upstream 100 bases:

>100_bases
GTTGCCAGAGTATCGAACCGAACTGGCCACCGACCGACGCGGCGACGACGGTAGCCGATCCCGCTGACGCCCCCCAATTC
CCAATTACGACAACGTCCTC

Downstream 100 bases:

>100_bases
GTCCGCAGGCAACCCCATAGCGACCTTCGCGACCACAGCGACCCCATATCATGCAAACCACACTGGCCGATTTCCTGCGC
AACACGCCCGAGGGCGAAGA

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVITARCGTPLAEIEDAVAQKRQ
MLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALRDFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPVPFDDATLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDA
SHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAPDDSAASADRVRAAAQAAGGH
ATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNPQRMYNGL

Sequences:

>Translated_367_residues
MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVITARCGTPLAEIEDAVAQKRQ
MLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALRDFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPVPFDDATLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDA
SHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAPDDSAASADRVRAAAQAAGGH
ATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNPQRMYNGL
>Mature_367_residues
MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVITARCGTPLAEIEDAVAQKRQ
MLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALRDFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPVPFDDATLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDA
SHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAPDDSAASADRVRAAAQAAGGH
ATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNPQRMYNGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=362, Percent_Identity=47.7900552486188, Blast_Score=317, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 39033; Mature: 39033

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVI
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEE
TARCGTPLAEIEDAVAQKRQMLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALR
EECCCCCHHHHHHHHHHHHHHEEECCCCCEEECCCCCCHHCHHHHHCCCCCCHHHHHHHH
DFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPVPFDDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHEEEEEEEEECCCCCC
TLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDA
HHEEHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEECCCCEEEEECCCCCCCCEECH
SHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAP
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHCCCCCCEEEECC
DDSAASADRVRAAAQAAGGHATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCH
QRMYNGL
HHHHCCC
>Mature Secondary Structure
MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVI
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEE
TARCGTPLAEIEDAVAQKRQMLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALR
EECCCCCHHHHHHHHHHHHHHEEECCCCCEEECCCCCCHHCHHHHHCCCCCCHHHHHHHH
DFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPVPFDDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHEEEEEEEEECCCCCC
TLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDA
HHEEHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEECCCCEEEEECCCCCCCCEECH
SHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAP
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHCCCCCCEEEECC
DDSAASADRVRAAAQAAGGHATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCH
QRMYNGL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]