Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 94311861

GI number: 94311861

Start: 3185602

End: 3186873

Strand: Direct

Name: glcF [H]

Synonym: Rmet_2929

Alternate gene names: 94311861

Gene position: 3185602-3186873 (Clockwise)

Preceding gene: 94311860

Following gene: 94311862

Centisome position: 81.1

GC content: 66.67

Gene sequence:

>1272_bases
ATGCAAACCACACTGGCCGATTTCCTGCGCAACACGCCCGAGGGCGAAGAGGCGAAATCCATCGTCGGCAAATGCGTGCA
CTGCGGTTTCTGCACGGCCACGTGCCCGACCTACCAGTTGCTCGGCGACGAACTCGACGGGCCGCGCGGCCGCATCTACC
TGATGAAGCAGGTGCTCGAGGGCAACCCCGTCACGGAAAGCACGCGCCTGCATCTGGATCGCTGCCTGACCTGCCGCAAT
TGCGAATCCACCTGCCCGTCTGGCGTGACCTATGGCCACCTGGTGGACATCGGCCGCAAGATCGTTGATGACCGGCTGGA
GGCGCAAGGCATCAAGCGCCCGGCCAAGGAACGCATCGTGCGCTGGGTGCTGCGCGAAGGCCTGACGCGCCCAGCGCTGT
TCGGCCCCGCACTGAGACTCGGCCAGATCGTGCGCCCCTGGCTGCCGCAGATGCTGCGCAACAAGGTACCCGCGAGCGAG
CCCGTGGCGGGCACCTGGCCGCGCACCGAGCATCCCCGCAAAATGCTGTTGCTCGACGGCTGCGTGCAACCGGCCATGTC
GCCCAACATCAATGCCGCCACGGCACGTGTGTTCGACAAACTCGGCGTACAACTGGTGGTGGCGCGGGAAGCCGGTTGTT
GTGGCGCCATCCGCTTCCACAATGGCGACCATCCAGGCGGGCTCGACAATATGCGCGCGAACATCGACGCATGGTGGCCG
CATATCGAGGCCGGAGCCGAAGCGATCGTCATGACCGCGTCGGGCTGCGGCGCGATGGTCAAGGACTACGGCCACCTGCT
GCGCAACGATCCGACGTACGCATCGCGCGCCGCGCGTGTATCGGCCATGACACGCGACTTGTCGGAGCTGCTGCCCGACT
TTACCGATGAACTGCACCGCCTGGCCGGCGCCGTGCCGCGCGATAGCCGCCGCGTGGCGTGGCATCCGCCGTGCACGCTG
CAGCACGGGCAACAGATCCGCGGCAAGGTCGAAGCGCTGCTGACCCGCTTGGGTGTGGACGTGCGCCTCTGCGCGGACAG
CCACCTGTGCTGCGGCTCCGCCGGCACCTACTCGGTGCTGCAGCCCGAACTGTCGTACAAGCTGCGCGACGACAAACTCG
CCAAGCTGCAGGCCACCTCGCCGGAATCCATCGTCACGGCCAATATCGGCTGCATCACGCATCTGCAAAGCGGCACGGAA
ACGCCGGTGATGCACTGGGTCGAGCTGGTGGACCGGATGCTGGCGCCGGTGGTGGCGGCGCAGGCGGCCTAA

Upstream 100 bases:

>100_bases
TCGACCCTGCGGGCATTTTCAATCCGCAGCGCATGTACAACGGACTCTGAGTCCGCAGGCAACCCCATAGCGACCTTCGC
GACCACAGCGACCCCATATC

Downstream 100 bases:

>100_bases
GCCGCGGGTGATGGCGGTCCCCCTCTCCCGCGAATGGGCGAGGGGAGCAGGAAGCCAACGGCAGCACTGACGTCCGGCTT
GCACGCATCTTGTACGGGAG

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNPVTESTRLHLDRCLTCRN
CESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIVRWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASE
PVAGTWPRTEHPRKMLLLDGCVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHRLAGAVPRDSRRVAWHPPCTL
QHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVLQPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTE
TPVMHWVELVDRMLAPVVAAQAA

Sequences:

>Translated_423_residues
MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNPVTESTRLHLDRCLTCRN
CESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIVRWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASE
PVAGTWPRTEHPRKMLLLDGCVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHRLAGAVPRDSRRVAWHPPCTL
QHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVLQPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTE
TPVMHWVELVDRMLAPVVAAQAA
>Mature_423_residues
MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNPVTESTRLHLDRCLTCRN
CESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIVRWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASE
PVAGTWPRTEHPRKMLLLDGCVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHRLAGAVPRDSRRVAWHPPCTL
QHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVLQPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTE
TPVMHWVELVDRMLAPVVAAQAA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=414, Percent_Identity=56.0386473429952, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=26.7782426778243, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 46351; Mature: 46351

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNPVTESTRLHLDRCLTCRNCESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASEPVAGTWPRTEHPRKMLLLDG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
CVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWP
HHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHCCCHHHCC
HIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHR
CHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGAVPRDSRRVAWHPPCTLQHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVL
HHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCHHC
QPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTETPVMHWVELVDRMLAPVVAA
CCHHHCEECCCHHHHEECCCCCCEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QAA
CCC
>Mature Secondary Structure
MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNPVTESTRLHLDRCLTCRNCESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASEPVAGTWPRTEHPRKMLLLDG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
CVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWP
HHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHCCCHHHCC
HIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHR
CHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGAVPRDSRRVAWHPPCTLQHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVL
HHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCHHC
QPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTETPVMHWVELVDRMLAPVVAA
CCHHHCEECCCHHHHEECCCCCCEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]