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The map label for this gene is panB

Identifier: 94311849

GI number: 94311849

Start: 3171044

End: 3171865

Strand: Direct

Name: panB

Synonym: Rmet_2917

Alternate gene names: 94311849

Gene position: 3171044-3171865 (Clockwise)

Preceding gene: 94311848

Following gene: 94311850

Centisome position: 80.73

GC content: 64.84

Gene sequence:

>822_bases
ATGAGTTACCTCCTCGATCCCTCCCGCAAGACCGTCACCATCCCCAAACTGCAGGCCATGCGTGAAGCCGGCGAAAAGAT
CGCCATGCTCACCGCCTACGATTCCAGCTTCTCCGCGCTGCTCGATCTCTGCGGCGTGGAAGTGATCCTGGTCGGCGATT
CGCTCGGCAACGTGATGCAGGGCCAGCAGACCACGCTGCCCGTCACGCTCGAGCACATGGTCTATCACACCGAATGCGTG
TCGCGCGGCAACCAGACCGCGCTGCTCGTATCCGACCTGCCGTTCGGCACGTATCCCACCCCCGAGAAGGCCTTCGAAAG
CGCCGCCGCGCTGATGCGTGCTGGCGCGCAGATGGTCAAGCTCGAAGGCGGAGACTGGCTGGCGCCGACGGTGAAGTTCC
TGGTCGAGCGCAGCATTCCGGTATGCGCGCATATCGGGCTGACACCGCAATCGGTACACGCGCTCGGCGGCTTCAAGGTG
CAGGGCAAGACCGACGAGAGCGCCGCGCAGCTGAAGCGTGACGCGCTGGCGCTGCAGCAAGCCGGCGCGCAGCTGATCGT
CATGGAGGCCGTGCCGGCCAAGCTCGCCGCCGAAGTGACGGCGCAACTCTCCATCCCGACCATCGGTATCGGCGCTGGTG
TCGATTGCTCGGGCCAGGTGCTGGTGCTGCAGGACATGATCAACATCTACCCGGGCCGCAAGGCCAAGTTCGTACGCAAC
TTCATGGAGGGACAGCCCTCGATCGAAGCGGCGATCCGCGCCTATGTGGCTGCTGTGAAGGACGGCAGCTTCCCGGCCGT
CGAGCACACGTTCTCGGCCTGA

Upstream 100 bases:

>100_bases
TGAAGCGGCTTTCGCCAGGCAGCACAACACACGAAAAAGACAGCGGTCGACCCTGGCCATCCGATGCACGGGTCCGAACC
GATCAACCACGGGTACTGCC

Downstream 100 bases:

>100_bases
ACCCCGCGTGGAGATCGGCGCCGCCACGCTCGAAGCATTCCGCCTGCTGGCCAGCGGCAACGGCGCCTTATGGTTCATTG
TCTGGACTTCGCTGATGGTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQGQQTTLPVTLEHMVYHTECV
SRGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKV
QGKTDESAAQLKRDALALQQAGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRN
FMEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA

Sequences:

>Translated_273_residues
MSYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQGQQTTLPVTLEHMVYHTECV
SRGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKV
QGKTDESAAQLKRDALALQQAGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRN
FMEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA
>Mature_272_residues
SYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQGQQTTLPVTLEHMVYHTECVS
RGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKVQ
GKTDESAAQLKRDALALQQAGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRNF
MEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=52.6717557251908, Blast_Score=283, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6319653, Length=272, Percent_Identity=36.7647058823529, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RALME (Q1LJ86)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585059.1
- ProteinModelPortal:   Q1LJ86
- SMR:   Q1LJ86
- STRING:   Q1LJ86
- GeneID:   4039745
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2917
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   AKFVRNF
- PhylomeDB:   Q1LJ86
- ProtClustDB:   PRK00311
- BioCyc:   RMET266264:RMET_2917-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29018; Mature: 28887

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 92-92 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQ
CCCCCCCCCCEEECHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCEEEEEECCCCHHHC
GQQTTLPVTLEHMVYHTECVSRGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVK
CCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKVQGKTDESAAQLKRDALALQQ
ECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRN
CCCEEEEEECCCHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEHHHHHCCCCHHHHHHH
FMEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA
HHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQ
CCCCCCCCCEEECHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCEEEEEECCCCHHHC
GQQTTLPVTLEHMVYHTECVSRGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVK
CCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKVQGKTDESAAQLKRDALALQQ
ECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRN
CCCEEEEEECCCHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEHHHHHCCCCHHHHHHH
FMEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA
HHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA