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The map label for this gene is miaA

Identifier: 94311841

GI number: 94311841

Start: 3163803

End: 3164750

Strand: Direct

Name: miaA

Synonym: Rmet_2909

Alternate gene names: 94311841

Gene position: 3163803-3164750 (Clockwise)

Preceding gene: 94311840

Following gene: 94311842

Centisome position: 80.54

GC content: 69.41

Gene sequence:

>948_bases
ATGTCCGTACAGTCCCACCCGCCCGTCGTCTGCCTGCTCGGCCCCACCGCATCGGGCAAGACGGCGGCCGCGCTCGAACT
GGCCGCACGCACGCCGGTCGAGATCATCAGCCTCGATTCAGCGCTGGTCTATCGCGAGATGGACATCGGCACGGCCAAGC
CCACGCACGAGGAACTGGCCAGCGTGCCGCATCACCTGATCGACATCATCGATCCGCTCGAAAGCTATTCGGCGGCGCAG
TTCATGACCGACGCCGAGCGGCTGATCGGCGAGATCCGCGCACGCGGCCGCACGCCGCTGATCGTCGGCGGCACGATGCT
CTATTACAAGGCGCTAACGCAAGGGCTCAACGACCTGCCTCAGGCCGATGCCACGGTGCGCGCGGAACTCGACGCGCTGG
CGGCGGAGCGAGGCTGGCCGGCGCTTCATGCGATGCTGGCCGAGGTCGACCCCATCACGGCGGCGCGGCTCGCGCCCAAC
GATGCCCAGCGCATCCAGCGCGCGCTGGAGATTCATCGCCTCAGCGGTCAGCCGATGTCCGCGCTGCTGGCACGGCAGGC
CGACGCCCGCACGTTCACCGGCGCGGCCGACGCACGCTACCGCGTGATCGCCCTGGAGCCGTCGGACCGCGCCGTGCTGC
ACGCGCGCATTGCCGACCGCTACGACGCGATGCTCAAGGGCGGGTTGATCGAGGAAGTGGAGCGGCTGCGCGCGCGCGGC
GACCTGCACCTGGGTCTGCCGTCAATCCGCTGCGTGGGCTATCGCCAGGTGTGGGAATACCTCGATGGCGACTGCGACTT
CGCCACGATGCGTGAGCGCGGCATCGCCGCCACGCGCCAGCTGTGCAAGCGACAGCTGACCTGGTTGCGCAGCACCCCGG
AGCGCATTGGCATCGACTGCCTGGCGCCGGACTACGTTGCAAGGGTTGCCGACGCGGCGGGGTGGTGA

Upstream 100 bases:

>100_bases
GCCGACCAGTGCAACCACGGCCGCCCGACATGGGTGCAGTTGACGGTTGCGGAACTCGACCGCCTGTTCCTGCGCGGCCA
GTAATCTCCCCAGCTTCAAG

Downstream 100 bases:

>100_bases
CGGCGTGCGGAGTGGACGATAATCGTCGCTCAAACCTTTTTGGCCTGCTTGCCGCCCGCCCCAGATGACCACGTTCACCA
TCCGCCCCGCCGCCCCCGCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELASVPHHLIDIIDPLESYSAAQ
FMTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLPQADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPN
DAQRIQRALEIHRLSGQPMSALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARG
DLHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDCLAPDYVARVADAAGW

Sequences:

>Translated_315_residues
MSVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELASVPHHLIDIIDPLESYSAAQ
FMTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLPQADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPN
DAQRIQRALEIHRLSGQPMSALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARG
DLHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDCLAPDYVARVADAAGW
>Mature_314_residues
SVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELASVPHHLIDIIDPLESYSAAQF
MTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLPQADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPND
AQRIQRALEIHRLSGQPMSALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARGD
LHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDCLAPDYVARVADAAGW

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=336, Percent_Identity=31.8452380952381, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=53.448275862069, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25144712, Length=278, Percent_Identity=27.6978417266187, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=324, Percent_Identity=28.0864197530864, Blast_Score=132, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24649830, Length=277, Percent_Identity=32.4909747292419, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RALME (Q1LJ94)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585051.1
- ProteinModelPortal:   Q1LJ94
- SMR:   Q1LJ94
- STRING:   Q1LJ94
- GeneID:   4039737
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2909
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q1LJ94
- ProtClustDB:   PRK00091
- BioCyc:   RMET266264:RMET_2909-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34490; Mature: 34359

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCHHHHEEECCCCCCCCCHHHHH
SVPHHLIDIIDPLESYSAAQFMTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCC
QADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPNDAQRIQRALEIHRLSGQPMS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARG
HHHHHHCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
DLHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDC
CEEECCCCHHEHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHCCCC
LAPDYVARVADAAGW
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
SVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELA
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCHHHHEEECCCCCCCCCHHHHH
SVPHHLIDIIDPLESYSAAQFMTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCC
QADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPNDAQRIQRALEIHRLSGQPMS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARG
HHHHHHCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
DLHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDC
CEEECCCCHHEHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHCCCC
LAPDYVARVADAAGW
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA