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The map label for this gene is hutC [H]

Identifier: 94311788

GI number: 94311788

Start: 3105744

End: 3106511

Strand: Reverse

Name: hutC [H]

Synonym: Rmet_2856

Alternate gene names: 94311788

Gene position: 3106511-3105744 (Counterclockwise)

Preceding gene: 94311789

Following gene: 94311787

Centisome position: 79.08

GC content: 66.15

Gene sequence:

>768_bases
ATGAACCAGTCCGCCAGCCAGGCACCCACCCCGACCAACGCCACCGCGCCTGCCGCGCTATATCGGCAGGTCAAGGAATA
CATCGCGCGCCAGATCCAGAGCGGCGTGTGGAAACCCGGTGACCGCGTGCCGTCCGAGCAGGAGCTCGTGAACCGCTTCT
CGGTATCGCGGATGACGGTCAATCGCGCGCTGCGCGAATTGTCGGAGCAGGGCAGGGTGGTGCGCGTGGCTGGTGTGGGG
ACGTTCGTGGCCGAGCAGAAGCCGCAGTCGACGCTGCTTTCCGTGGTCAACCTGCAGGACGAGATCCGTATGCGTGGGCA
TGACTACGCGTGCGACGTGATCCTGGTGGAGCGCGTCTCCGCGCCGATCGAGGTCGCCGCCGCACTGGAGTTGCACACGG
GCGAGTCGGTGTACCACTCGGTTTGTGTGCATCGCGAGGACGGCGTGCCAGTTCAGCTCGAAGACCGCTACGTGAACCCG
CGCGTGGCGCCAGATTTCATCAACCAGGATTTCAGCGGCACCAAGCCCGGCGAATACCTGTTGCGCAATGTGCCGTACGA
CCAGGTGGAGCATGTGGTCGATGCGATCTCCGCCACGCCCGAGCAGGCGGCGCAGCTTGAGATGCCCCCGACGCAGCCGT
GCCTGCTGCTGACGCGCCGCACATGGACCAACGGCGTGCCGGTGACGTTCGTGCGTTGCCTGCACCCGGGCAACCGCTAC
CGGCTTGGCAGCCGCTTCCGTGCCGACGGCAATCCTGCCTTCGGCTGA

Upstream 100 bases:

>100_bases
CACTACACGATCAAGACCTCTCCCGAGTTCTCCGCGCTGAAGGCACGGCTGACCGAGGAGATTCGCGCCGAAGCCATGGC
CTCGGCCGAGCATTGAGACC

Downstream 100 bases:

>100_bases
TATTTGCGCCTGAGGCCAGCAACAGCTGGTCATCGGGCTTATTTTTCGACTAACCTGTATAGACAGGCCCATACAAGGTC
TTTATCCAAGACCGACAAGC

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTVNRALRELSEQGRVVRVAGVG
TFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVSAPIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNP
RVAPDFINQDFSGTKPGEYLLRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRY
RLGSRFRADGNPAFG

Sequences:

>Translated_255_residues
MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTVNRALRELSEQGRVVRVAGVG
TFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVSAPIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNP
RVAPDFINQDFSGTKPGEYLLRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRY
RLGSRFRADGNPAFG
>Mature_255_residues
MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTVNRALRELSEQGRVVRVAGVG
TFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVSAPIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNP
RVAPDFINQDFSGTKPGEYLLRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRY
RLGSRFRADGNPAFG

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=198, Percent_Identity=28.2828282828283, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082252, Length=238, Percent_Identity=27.3109243697479, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790118, Length=156, Percent_Identity=27.5641025641026, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 28359; Mature: 28359

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NRALRELSEQGRVVRVAGVGTFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVS
HHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCC
APIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNPRVAPDFINQDFSGTKPGEYL
CCHHHHHEEEECCCHHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHCCCCCCCCCHHHH
LRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRY
HHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCE
RLGSRFRADGNPAFG
ECCCEECCCCCCCCC
>Mature Secondary Structure
MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NRALRELSEQGRVVRVAGVGTFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVS
HHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCC
APIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNPRVAPDFINQDFSGTKPGEYL
CCHHHHHEEEECCCHHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHCCCCCCCCCHHHH
LRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRY
HHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCE
RLGSRFRADGNPAFG
ECCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203753 [H]