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The map label for this gene is cysI [H]

Identifier: 94311748

GI number: 94311748

Start: 3062895

End: 3064577

Strand: Reverse

Name: cysI [H]

Synonym: Rmet_2816

Alternate gene names: 94311748

Gene position: 3064577-3062895 (Counterclockwise)

Preceding gene: 94311749

Following gene: 94311747

Centisome position: 78.02

GC content: 63.64

Gene sequence:

>1683_bases
ATGTATCAGTACGATCAATATGACCAGCGTATCGTCCAGGAGCGCGTTGCCCAGTTCCGTGACCAGGTGCGTCGCCGCCT
GTCCGATGAGCTGACCGAGGAAGAATTCCTGCCGCTGCGCCTGCAGAACGGCCTGTACATGCAGCGTCACGCCTACATGC
TGCGCGTGGCGATTCCGTACGGCCTGCTGGCCTCGAAGCAGATGCGCATGCTGGCCCATATCGCCCGCACCTACGACCGC
GACTACGGCCATTTCTCCACGCGCCAGAACATCCAGTACAACTGGATGGATCTGGAGCGCGTGCCCGACGTGCTGGACGA
CCTCGCCAGCGTCGAAATGCACGGCATCCAGACCTCCGGCAACTGCGTGCGCAACATCACCACCGACCAGTTCGCCGGCG
TGGCGCCGGACGAGACGGTGGACCCGCGCGTGCTGGCCGAACTGCTGCGCCAGTGGAGCACGTTCCAGCCCGAATTCGCG
TTCCTGCCGCGCAAGTTCAAGATCGCCATCTCGTCGTCGGCCGATGACCGCGCCGTGGTGCAGATGCACGATATCGGCAT
CTATGCGTACAAGAACGCCGCTGGCGAGACGCGCCTGCGCATCCTTGCTGGTGGAGGTCTGGGCCGCACGCCGATTCTGG
GCACGATCATCAAGGAAGATCTGCCGTGGCAGCACATGCTGACCTATGTGGAGTCCGCGATCCGCGTGTACAACCGCTAC
GGCCGCCGCGACAACAAGTACAAGGCCCGCATCAAGATCTTGGTGAAGGCCATCGGCGCCGAGAAGTTCGCCGCCGAAGT
GGAAGAAGAGTGGAAGTTCAGCAAGGACGGCCCGGGCACGCTGACGCAGGCCGAGTTCGATCGCGTGGCGCAGTACTTCC
AGCCGCCCGCCTACGAAAAGCTGCCCGACACCGACGCATCGTTCGAAAAGCACCTGCTCGAGAACAAGGCGTTCGCACGC
TGGGTCAACCGCAACGTGCACGCGCACAAGGTGCCGGGCTATGCATCCGTGACGCTGTCGACCAAGCCGGGTCCGGTGTC
GCCCCCGGGCGATGCCACGTCCGCGCAAATGGACACGGTTGCCGATTTCGCTGATCGCTATGGCTATGGCGAACTGCGCG
TGGCGCATGAGCAGAACCTGGTGCTGCCCGATGTGAAAAAGCGTGACCTGTTCGCGCTGTGGGAAGACGCGAAGAAGGCC
GGTCTGGCCACGGCCAACATCGGCCTGCTGACCGACATCATCGCTTGCCCGGGCGGCGACTTCTGCTCGCTGGCCAACGC
CAAGTCGATTCCGATCGCGCTGGCGATCCAGGAGCGCTTCGACAACCTCGACTACGTCTACGACCTCGGCGACATCTCGC
TGAACATCTCGGGTTGCATCAACGCCTGCGGCCACCACCACGTCGGCAACATCGGCGTGCTGGGCGTCGACAAGGATGGC
GCCGAGTGGTACCAGGTCACGATCGGCGGCGCCCAGGGCAATGACACGGCGCTGGGCAAGGTGATCGGCCCGTCGTTCAG
CGCCCACGAGATGCCCGATGTGGTGTCGCGCATCATCGAGACATTCGTGGCCAACCGTGTTGGCGACGAGCGCTTTATCG
AAACCGTTGGCCGGATTGGCATCGCGCCGTTCAAGGAACGGGTGTACGCGGACAAGCAGCGCGAAGAGCGCGCCGAAGCC
TGA

Upstream 100 bases:

>100_bases
ATGAATGATTCAGGCGTCGTTACACGGCCTTGCCAGAACCGCTTCGCTTCATTCCTGACCAGCCTCCCTAAAGTTACCGA
ATACTGAACGGACCCACACC

Downstream 100 bases:

>100_bases
CGAACGGAGTTACCGAACATGGCAAAGATCATTCAACTGCAACGCGACGCCGCCGAATTCCGTCCTGTCATCGTCGACGA
TAGCTGGACCGTGCTGCGTG

Product: sulfite reductase, subunit beta (hemoprotein with two 4Fe-4S domains)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MYQYDQYDQRIVQERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQMRMLAHIARTYDR
DYGHFSTRQNIQYNWMDLERVPDVLDDLASVEMHGIQTSGNCVRNITTDQFAGVAPDETVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISSSADDRAVVQMHDIGIYAYKNAAGETRLRILAGGGLGRTPILGTIIKEDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAAEVEEEWKFSKDGPGTLTQAEFDRVAQYFQPPAYEKLPDTDASFEKHLLENKAFAR
WVNRNVHAHKVPGYASVTLSTKPGPVSPPGDATSAQMDTVADFADRYGYGELRVAHEQNLVLPDVKKRDLFALWEDAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDYVYDLGDISLNISGCINACGHHHVGNIGVLGVDKDG
AEWYQVTIGGAQGNDTALGKVIGPSFSAHEMPDVVSRIIETFVANRVGDERFIETVGRIGIAPFKERVYADKQREERAEA

Sequences:

>Translated_560_residues
MYQYDQYDQRIVQERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQMRMLAHIARTYDR
DYGHFSTRQNIQYNWMDLERVPDVLDDLASVEMHGIQTSGNCVRNITTDQFAGVAPDETVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISSSADDRAVVQMHDIGIYAYKNAAGETRLRILAGGGLGRTPILGTIIKEDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAAEVEEEWKFSKDGPGTLTQAEFDRVAQYFQPPAYEKLPDTDASFEKHLLENKAFAR
WVNRNVHAHKVPGYASVTLSTKPGPVSPPGDATSAQMDTVADFADRYGYGELRVAHEQNLVLPDVKKRDLFALWEDAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDYVYDLGDISLNISGCINACGHHHVGNIGVLGVDKDG
AEWYQVTIGGAQGNDTALGKVIGPSFSAHEMPDVVSRIIETFVANRVGDERFIETVGRIGIAPFKERVYADKQREERAEA
>Mature_560_residues
MYQYDQYDQRIVQERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQMRMLAHIARTYDR
DYGHFSTRQNIQYNWMDLERVPDVLDDLASVEMHGIQTSGNCVRNITTDQFAGVAPDETVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISSSADDRAVVQMHDIGIYAYKNAAGETRLRILAGGGLGRTPILGTIIKEDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAAEVEEEWKFSKDGPGTLTQAEFDRVAQYFQPPAYEKLPDTDASFEKHLLENKAFAR
WVNRNVHAHKVPGYASVTLSTKPGPVSPPGDATSAQMDTVADFADRYGYGELRVAHEQNLVLPDVKKRDLFALWEDAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDYVYDLGDISLNISGCINACGHHHVGNIGVLGVDKDG
AEWYQVTIGGAQGNDTALGKVIGPSFSAHEMPDVVSRIIETFVANRVGDERFIETVGRIGIAPFKERVYADKQREERAEA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 63000; Mature: 63000

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQYDQYDQRIVQERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCEEEHHHHEEEEEECCH
GLLASKQMRMLAHIARTYDRDYGHFSTRQNIQYNWMDLERVPDVLDDLASVEMHGIQTSG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCC
NCVRNITTDQFAGVAPDETVDPRVLAELLRQWSTFQPEFAFLPRKFKIAISSSADDRAVV
CEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCCCEEE
QMHDIGIYAYKNAAGETRLRILAGGGLGRTPILGTIIKEDLPWQHMLTYVESAIRVYNRY
EEEECEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKAIGAEKFAAEVEEEWKFSKDGPGTLTQAEFDRVAQYFQPPAYEK
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
LPDTDASFEKHLLENKAFARWVNRNVHAHKVPGYASVTLSTKPGPVSPPGDATSAQMDTV
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHH
ADFADRYGYGELRVAHEQNLVLPDVKKRDLFALWEDAKKAGLATANIGLLTDIIACPGGD
HHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
FCSLANAKSIPIALAIQERFDNLDYVYDLGDISLNISGCINACGHHHVGNIGVLGVDKDG
CCCCCCCCCCCEEEEEHHHCCCCCEEEECCCEEEEHHHHHHHCCCCCCCCEEEEEECCCC
AEWYQVTIGGAQGNDTALGKVIGPSFSAHEMPDVVSRIIETFVANRVGDERFIETVGRIG
CCEEEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
IAPFKERVYADKQREERAEA
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQYDQYDQRIVQERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCEEEHHHHEEEEEECCH
GLLASKQMRMLAHIARTYDRDYGHFSTRQNIQYNWMDLERVPDVLDDLASVEMHGIQTSG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCC
NCVRNITTDQFAGVAPDETVDPRVLAELLRQWSTFQPEFAFLPRKFKIAISSSADDRAVV
CEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCCCEEE
QMHDIGIYAYKNAAGETRLRILAGGGLGRTPILGTIIKEDLPWQHMLTYVESAIRVYNRY
EEEECEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKAIGAEKFAAEVEEEWKFSKDGPGTLTQAEFDRVAQYFQPPAYEK
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
LPDTDASFEKHLLENKAFARWVNRNVHAHKVPGYASVTLSTKPGPVSPPGDATSAQMDTV
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHH
ADFADRYGYGELRVAHEQNLVLPDVKKRDLFALWEDAKKAGLATANIGLLTDIIACPGGD
HHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
FCSLANAKSIPIALAIQERFDNLDYVYDLGDISLNISGCINACGHHHVGNIGVLGVDKDG
CCCCCCCCCCCEEEEEHHHCCCCCEEEECCCEEEEHHHHHHHCCCCCCCCEEEEEECCCC
AEWYQVTIGGAQGNDTALGKVIGPSFSAHEMPDVVSRIIETFVANRVGDERFIETVGRIG
CCEEEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
IAPFKERVYADKQREERAEA
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA