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The map label for this gene is cysG [H]

Identifier: 94311743

GI number: 94311743

Start: 3058372

End: 3059136

Strand: Reverse

Name: cysG [H]

Synonym: Rmet_2811

Alternate gene names: 94311743

Gene position: 3059136-3058372 (Counterclockwise)

Preceding gene: 94311744

Following gene: 94311739

Centisome position: 77.88

GC content: 68.5

Gene sequence:

>765_bases
ATGGACAGGCAGCACGGCAAGGCCACGGGCAAGGTGTACCTGATTGGTGCGGGTCCCGGCGCGGCCGATCTCATTACGGT
GCGCGGCGCACGACTGTTGGGCGAGGCGCAGGTCGTGCTGCACGATGCACTCGTATCGCCCGAGATGCTGGCGTGGTGTC
CGCAGGCCGAACTGGTCGAGGTGGGCAAGCGCTGCGGCCAGCGTTCCACGGCGCAGCTCTTCATCAATCGCCAGATCGTC
GACCTGGCCACAAAGCACGAGCGCGTGGTGCGGCTCAAGGGCGGCGACCCGATGCTGTTTGGCCGCGCCGACGAGGAGTT
GCAGGCGCTGGAAGCGGCCGGTATCGAGTACGAGGTGGTGCCCGGCATCACCGCGGCGCTCGCGGCGGCCAGCGCGATCG
GCCGGCCCTTGACCAAGCGTGGTGTATCGCGCAGCGTGGCGTTCGCTACCCAGGCCAAGGCGGCCGATGCCGTCGACGTG
GAGGCCGATGTGAAGGCAGACACGATCATCTACTACATGGGCCGTGATCAGGCGGCGAACATTGCGACGCAGTTGATCGC
TCGCGGCAAGCCGGCGTCGACACCCGCATGGGTAGTGGAAGCCGCTACGACGCCGCGCCAGCGCAGCCACCAGTTCACGC
TGGGCCAGATGGCGGCAGGCGAGGCGGCACGCTGGATGGACCCGGTGCAGCCCAGCCTGCTGATGATCGGCGAGGCCCTG
GGGAGCAGGGCGACGGAGTACGTGGAAGCCGAACGCGCCGCGTGA

Upstream 100 bases:

>100_bases
GCTGATCGACGAAGCCACCAACCACACGGTGGCGGCGGGCATGATCCGTGCGTATGCCTGATATCGTCTGAGTCGGGTAC
TTCATACAGCGAGGGTCAGG

Downstream 100 bases:

>100_bases
TCGGTTTGCCCGGTGGAAAAAAGGCCAGAGATTGACTCTGGCCTTTTTGCATTTTGTGTCCCGGGCTAATGTCCAGAATC
GGGGGCGGGAGCGGGAGAAA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVEVGKRCGQRSTAQLFINRQIV
DLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVVPGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDV
EADVKADTIIYYMGRDQAANIATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEAL
GSRATEYVEAERAA

Sequences:

>Translated_254_residues
MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVEVGKRCGQRSTAQLFINRQIV
DLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVVPGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDV
EADVKADTIIYYMGRDQAANIATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEAL
GSRATEYVEAERAA
>Mature_254_residues
MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVEVGKRCGQRSTAQLFINRQIV
DLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVVPGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDV
EADVKADTIIYYMGRDQAANIATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEAL
GSRATEYVEAERAA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=237, Percent_Identity=43.0379746835443, Blast_Score=161, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=28.1512605042017, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 26982; Mature: 26982

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVE
CCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHH
VGKRCGQRSTAQLFINRQIVDLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVV
HHHHHCCCCCCHHEEHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEC
PGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDVEADVKADTIIYYMGRDQAAN
CCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHH
IATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEAL
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHCCCHHHHCCCCCHHHHHHHHHH
GSRATEYVEAERAA
HHHHHHHHHHHCCC
>Mature Secondary Structure
MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVE
CCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHH
VGKRCGQRSTAQLFINRQIVDLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVV
HHHHHCCCCCCHHEEHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEC
PGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDVEADVKADTIIYYMGRDQAAN
CCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHH
IATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEAL
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHCCCHHHHCCCCCHHHHHHHHHH
GSRATEYVEAERAA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA