Click here to switch to the map view.

The map label for this gene is cysN [H]

Identifier: 94311744

GI number: 94311744

Start: 3059176

End: 3060480

Strand: Reverse

Name: cysN [H]

Synonym: Rmet_2812

Alternate gene names: 94311744

Gene position: 3060480-3059176 (Counterclockwise)

Preceding gene: 94311745

Following gene: 94311743

Centisome position: 77.91

GC content: 64.6

Gene sequence:

>1305_bases
ATGTCTCACCAATCCCAACATCAAGGCCTGCTGCGTTTCATCACCGCGGGCTCCGTGGACGACGGCAAGAGCACGCTGAT
CGGCCGTCTGTTGTTTGACAGCAAGGCAGTGCTGTCCGACCAGCTCACCGCGCTGGCCAACGCCAAGAACAAGCGCACCG
CTGGCGAGCAGATCGACTTCTCGCTGCTGACCGACGGCCTCGAGGCCGAGCGAGAGCAGGGCATCACGATCGACGTGGCT
TACCGCTATTTCTCGACCGCGCGCCGCAAGTTCATCATTGCCGATACGCCGGGCCACGAGCAGTACACGCGCAACATGGT
GACGGGCGCCTCGACTGCGCACGCCGCCATCGTGCTGGTGGATGCCACCCGCGTCACGGTCAAGGACGGCCGCGCCGAGC
TGCTGGCCCAGACCAAGCGTCACTCGGCAATCCTGAAGTTGCTGGAACTGCAGCACGTGATCGTCGCCGTGAACAAGATG
GACCTGGTTGACTACAGCGAGCAGCGCTTCAACGAGATCCGCGCCGCCTACATCGAACTGGCCGCGCAACTGGGCCTGAA
GGATGTGACCTACGTGCCGGTGTCGGCACTGCGTGGCGACAACATCGTCCATGCCAGCGACGCCATGCCGTGGTACCAGG
GCGAGCCGCTGCTGCCGCTGCTGGAATCGCTGCCGGTGGAAGATGTGGCGCCGGTAGGCGATGCCGCGCTGCGCTTCCCG
GTGCAACTGGTGGTTCGCCAGGACGGTTCCGCCGCCGACGACTTCCGTGGCTACGCTGGCCGTGTGGAAGCCGGCACCGT
GCGCGTGGGCCAGAAGCTGCGCGTGCTGCCCGCCAATCGCGAAGCCGTGGTGGCCGAAGTGCTGACGCCGAATGGATCGG
CCGATTCCGCCAGCGCCGGCGACACCGTCACGGTGCGTCTGGCCGAAGACGTTGATGTATCGCGTGGTGACATGTTCGTG
TCGGCCGATACCGCCGCCGAATCGGCGAAGAAGCTGACTGCCGACCTGTGCTGGTTCGACGATGAGTCGCTGAATCCGTC
GCGCAAGTACGTGCTCAAGCACACTACGGCCAGCGTGTTCGCGCGCGTGTCGGCGGTGGATCGCGTGCTGGATGTGCATA
CGCTGTCGCACGAAACCGGCCGCCATGAGATCAACCTGAACGACATCGGTTCGGTGCAGATCTCGCTGCAGAAGCCGATC
GTCTGCGATACATACGGCGACAATCCGGCGACGGGCGCGTTCGTGCTGATCGACGAAGCCACCAACCACACGGTGGCGGC
GGGCATGATCCGTGCGTATGCCTGA

Upstream 100 bases:

>100_bases
CGCGGCGCCACCCGCATGGACGACCAGACGAGCGAAGCGTCGATGGAACGCCGGAAAAAAGAAGGCTACTTCTAAATCGA
CGCAGCAGAGGAATACGACA

Downstream 100 bases:

>100_bases
TATCGTCTGAGTCGGGTACTTCATACAGCGAGGGTCAGGATGGACAGGCAGCACGGCAAGGCCACGGGCAAGGTGTACCT
GATTGGTGCGGGTCCCGGCG

Product: sulfate adenylyltransferase, subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDFSLLTDGLEAEREQGITIDVA
YRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLVDATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKM
DLVDYSEQRFNEIRAAYIELAAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFP
VQLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAGDTVTVRLAEDVDVSRGDMFV
SADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVFARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPI
VCDTYGDNPATGAFVLIDEATNHTVAAGMIRAYA

Sequences:

>Translated_434_residues
MSHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDFSLLTDGLEAEREQGITIDVA
YRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLVDATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKM
DLVDYSEQRFNEIRAAYIELAAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFP
VQLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAGDTVTVRLAEDVDVSRGDMFV
SADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVFARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPI
VCDTYGDNPATGAFVLIDEATNHTVAAGMIRAYA
>Mature_433_residues
SHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDFSLLTDGLEAEREQGITIDVAY
RYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLVDATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKMD
LVDYSEQRFNEIRAAYIELAAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFPV
QLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAGDTVTVRLAEDVDVSRGDMFVS
ADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVFARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPIV
CDTYGDNPATGAFVLIDEATNHTVAAGMIRAYA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=28.8329519450801, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=28.8329519450801, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI194018522, Length=424, Percent_Identity=26.8867924528302, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI46094014, Length=424, Percent_Identity=27.3584905660377, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI194018520, Length=424, Percent_Identity=26.8867924528302, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI194097354, Length=424, Percent_Identity=26.8867924528302, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=27.5938189845475, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=28.5714285714286, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI34147630, Length=311, Percent_Identity=29.2604501607717, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789108, Length=427, Percent_Identity=48.711943793911, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI2367247, Length=360, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=25.4901960784314, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=25.4901960784314, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115532067, Length=337, Percent_Identity=33.5311572700297, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI115532065, Length=337, Percent_Identity=33.5311572700297, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=29.0540540540541, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=29.0540540540541, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=441, Percent_Identity=27.437641723356, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=27.4774774774775, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=31.7241379310345, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=219, Percent_Identity=29.2237442922374, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=24.0601503759398, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=30.1762114537445, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325337, Length=468, Percent_Identity=27.991452991453, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=468, Percent_Identity=27.991452991453, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=439, Percent_Identity=28.0182232346241, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324761, Length=296, Percent_Identity=28.7162162162162, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=28.9237668161435, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=28.9237668161435, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=28.4116331096197, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=28.4116331096197, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=28.4116331096197, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=28.4116331096197, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17137380, Length=428, Percent_Identity=27.1028037383178, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=28.2758620689655, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI281363316, Length=314, Percent_Identity=28.6624203821656, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864358, Length=314, Percent_Identity=28.6624203821656, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921738, Length=159, Percent_Identity=32.0754716981132, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 47006; Mature: 46874

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00469 NDP_KINASES ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDF
CCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCH
SLLTDGLEAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLV
HHHHCCHHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEE
DATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKMDLVDYSEQRFNEIRAAYIEL
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCCCCHHHHHHHHHHHHHH
AAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFP
HHHCCCCCEEEEEHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCE
VQLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAG
EEEEEECCCCCCHHHCCCCCCEECCEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCC
DTVTVRLAEDVDVSRGDMFVSADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVF
CEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHEEECCCCCCCCCHHEEEEEHHHHHH
ARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPIVCDTYGDNPATGAFVLIDEA
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCCCCEEEEEECC
TNHTVAAGMIRAYA
CCCCHHHHHHHHCC
>Mature Secondary Structure 
SHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDF
CCCHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCH
SLLTDGLEAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLV
HHHHCCHHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEE
DATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKMDLVDYSEQRFNEIRAAYIEL
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCCCCHHHHHHHHHHHHHH
AAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFP
HHHCCCCCEEEEEHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCE
VQLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAG
EEEEEECCCCCCHHHCCCCCCEECCEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCC
DTVTVRLAEDVDVSRGDMFVSADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVF
CEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHEEECCCCCCCCCHHEEEEEHHHHHH
ARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPIVCDTYGDNPATGAFVLIDEA
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCCCCEEEEEECC
TNHTVAAGMIRAYA
CCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA