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The map label for this gene is epsM [H]

Identifier: 94311657

GI number: 94311657

Start: 2961081

End: 2961626

Strand: Reverse

Name: epsM [H]

Synonym: Rmet_2725

Alternate gene names: 94311657

Gene position: 2961626-2961081 (Counterclockwise)

Preceding gene: 94311658

Following gene: 94311656

Centisome position: 75.4

GC content: 48.72

Gene sequence:

>546_bases
TTGGGAAAGCGTCATTTTTTTGATGATGCTTGGCCCAATATTAATATGAATGGCCGCTGGCAGGTGGTCGGTGATTTTTC
GGCCTTACTTGCATATGCGCAAGTATTCGATGCCGCATTTGTCTCGATAGGAAATTGTCGTGTGCGATGGGCGAAGCACC
AAGCCTTGCTTGCTGCTGGCATACCGATAGCCACTGTCATACATCCGCGTGCGTACATAAGTCAATTTGCACGCTTAGGT
ATTGGAAGCATTGCGATGGCAGGGGTTGTTATCAATGCTGATGTTGGTGACGGAGGCATTGTAAACACTGATGCCACCAT
AGATCATGACTGCATATTGGATAATGGAGTACATGTCAGCCCGGGAGCGCATCTAAGCGGAAGCGTAAGTGTTGGAAAAT
GTTCATGGATTGCTGTTGGTGCTTCGATAAAACAGGGTATCACGGTAGGTTCTGATGCAATTGTTGGTGCAGGTGCTGTA
GTTGTGAGACCAGTTCGAGATGGCGTGACAGTGATGGGTTGTCCCGCACGGGAGCAGAGAGTCTAG

Upstream 100 bases:

>100_bases
GACAATGCCGAAGTTCACGGGGAAGAATGAATAAACGATTCGCCCTCTTTGGCGCCAGCGGCCATGGCAAAGTTGTAGCC
GACGCAGGATTGGAGGCTGG

Downstream 100 bases:

>100_bases
GCGACTCTTGGGAACCTATAATACAGTTGAAGAAAATATCATCTGATTTCTAATTCTACTTATGCTGAACACTTCATTTT
CTCCGTGGCCTAGTTTCACC

Product: Transferase (hexapeptide repeat)

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MGKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAGIPIATVIHPRAYISQFARLG
IGSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVSPGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAV
VVRPVRDGVTVMGCPAREQRV

Sequences:

>Translated_181_residues
MGKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAGIPIATVIHPRAYISQFARLG
IGSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVSPGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAV
VVRPVRDGVTVMGCPAREQRV
>Mature_180_residues
GKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAGIPIATVIHPRAYISQFARLGI
GSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVSPGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAVV
VRPVRDGVTVMGCPAREQRV

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 18874; Mature: 18743

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAG
CCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEHHHEEECCC
IPIATVIHPRAYISQFARLGIGSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVS
CCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCEEECCCEEEC
PGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAVVVRPVRDGVTVMGCPAREQR
CCCCCCCCEEECCEEEEEECCHHHCCEEECCCCEECCCEEEEEECCCCEEEEECCCHHCC
V
C
>Mature Secondary Structure 
GKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAG
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEHHHEEECCC
IPIATVIHPRAYISQFARLGIGSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVS
CCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCEEECCCEEEC
PGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAVVVRPVRDGVTVMGCPAREQR
CCCCCCCCEEECCEEEEEECCHHHCCEEECCCCEECCCEEEEEECCCCEEEEECCCHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]