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The map label for this gene is kdtA [H]

Identifier: 94311646

GI number: 94311646

Start: 2946890

End: 2948164

Strand: Reverse

Name: kdtA [H]

Synonym: Rmet_2714

Alternate gene names: 94311646

Gene position: 2948164-2946890 (Counterclockwise)

Preceding gene: 94311647

Following gene: 94311645

Centisome position: 75.05

GC content: 67.69

Gene sequence:

>1275_bases
ATGCTGCGTTTCGTCTATTCGTTGCTGTGGCTGCTGATCTTGCCACTGGCGTTGCTGCGGCTGGTCTGGCGTTCGCGCAA
GGAGTCGGGCTACATCCAGCATGTTGGCGAGCGGCTGGGGCGCTATGGCGATCTATCGGCCGCCGGCCCATGGCTGTGGG
TCCACGCGGTGTCCGTTGGAGAAACGCGGGCGGCGCAACCGCTGATCGACGCGCTGCTGGCGGCGTATCCGCGGCACCGG
CTGCTGCTGACGCACATGACGCCGACCGGTCGTCAGACTGGCGCGCAGCTCTACGGTGCCAACCCACGCGTGCAGCAGTG
CTACCTGCCGTATGACCTGCCCTGGCTGGTCCGACGCTTTCTTGCCTACTTCCAGCCGGATGCCGGCCTGATCATGGAAA
CGGAAGTCTGGCCGAACCTGGTGCACGTCACGCGTCGCATGGGCGTGCCACTGTTCCTGGTCAACGCTCGTCTGTCGCCG
CGCAGCTATCGGCGTACCGCGCGATTCGGCGGTGCCGCCGCAGCGCTGTACAACGACTTCACGATGGTGCTGGCCCAGAC
CGCTGGTGACGCGGAACGCTATCGCGCGTTGGGCGTGAAGTCGGTGCAGGTCACGGGCAACCTGAAGTTCGATATGCAAC
CGCCCGAGGCCGGCGTGGCACGCGGACAGCTTCTGCGGCAGGCTTTCGGCAACCGGCATGTGCTGGCGGCAGCGAGCACG
CGCGAGGGCGAGGAGCCGTTGATCCTGGAGGCATTCTCGCGCTGGCCGGGCACGGATCGCCCCGCATTGCTGCTGGTGCC
GCGCCATCCGCAGCGCTTTGACGAGGTGGCCGCGATGGCGACGCGCGCCGGCTTCAGCGTGCAGCGGCGCAGCGCGCTCG
ATATTGAGCAATTGTCGGCGCCGATTACCGCCGACGTCGTGCTTGGTGATTCGATGGGCGAGATGGCGATGTACTACGCG
GCCTCGGACATCGCGTATATCGGCGGCAGCCTGATCCCGCTCGGCGGTCAGAACCTGATCGAGGCCTGCGCCGTGGGCAC
GCCGGTGCTGATGGGGCCACACACGTTCAACTTCGCGCAGGCGACCGAGGACGCCATTGCCGCGGGCGCGTGCCAGCGCG
TGGCCGATGCGGACGAACTGATGGTTGCCGCTGCTGCCATCCTCGGTGACGCAACGCGGCTTGCGGACATGCGCGAGCAC
GCGCTGACCTTCGCCGGCCTCCATCGTGGCGCGACGGCACGCACGCTGGGCGCGCTGGCGCCGGTGTTGCGCTGA

Upstream 100 bases:

>100_bases
ACTGGTCCGACCGCATTGCCAATGTCGGTGACGATGGCATCGTGCCGACCGTCGACGAAGTCCATGATGCGGCGATCCGC
GTGCGCGGAGTGACTGCCTG

Downstream 100 bases:

>100_bases
TGCGGCGTAAGCGGCGTTCCACTAGACTGAGTGTTGCCTGAGGCTCTTCAAGCGCTTGCGTTCGCTCCCGTATGTCTCCC
ATACGGCGAGAAGGGCGCAA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVGETRAAQPLIDALLAAYPRHR
LLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRFLAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSP
RSYRRTARFGGAAAALYNDFTMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAAST
REGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSAPITADVVLGDSMGEMAMYYA
ASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREH
ALTFAGLHRGATARTLGALAPVLR

Sequences:

>Translated_424_residues
MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVGETRAAQPLIDALLAAYPRHR
LLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRFLAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSP
RSYRRTARFGGAAAALYNDFTMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAAST
REGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSAPITADVVLGDSMGEMAMYYA
ASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREH
ALTFAGLHRGATARTLGALAPVLR
>Mature_424_residues
MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVGETRAAQPLIDALLAAYPRHR
LLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRFLAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSP
RSYRRTARFGGAAAALYNDFTMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAAST
REGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSAPITADVVLGDSMGEMAMYYA
ASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREH
ALTFAGLHRGATARTLGALAPVLR

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=431, Percent_Identity=40.1392111368909, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46321; Mature: 46321

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETRAAQPLIDALLAAYPRHRLLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRF
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHH
LAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSPRSYRRTARFGGAAAALYNDF
HHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
TMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAAST
HHHHHHHCCCHHHHHHHCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECC
REGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSA
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHCC
PITADVVLGDSMGEMAMYYAASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQ
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCEEECCCCCCHHH
ATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREHALTFAGLHRGATARTLGALA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVLR
HHCC
>Mature Secondary Structure
MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETRAAQPLIDALLAAYPRHRLLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRF
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHH
LAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSPRSYRRTARFGGAAAALYNDF
HHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
TMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAAST
HHHHHHHCCCHHHHHHHCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECC
REGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSA
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHCC
PITADVVLGDSMGEMAMYYAASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQ
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCEEECCCCCCHHH
ATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREHALTFAGLHRGATARTLGALA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVLR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]