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The map label for this gene is thyA

Identifier: 94311504

GI number: 94311504

Start: 2793653

End: 2794447

Strand: Direct

Name: thyA

Synonym: Rmet_2568

Alternate gene names: 94311504

Gene position: 2793653-2794447 (Clockwise)

Preceding gene: 94311503

Following gene: 94311505

Centisome position: 71.12

GC content: 61.13

Gene sequence:

>795_bases
ATGAAACAGTACCTCGACTTCATGCGCCATGTGTACGAGCATGGCACCGAAAAGTCCGACCGCACCGGCACCGGTACGCG
CTCGGTCTTCGGCTACCAGATGCGCTTCGACCTGAACGAGGGCTTTCCCGTCGTCACCACGAAAAAGCTGCACCTGAAGT
CGATCATCCATGAACTGCTGTGGTTCCTGCAGGGCTCGACCAACATCAAGTACCTGAAGGACAACGGCGTCACCATCTGG
GACGAATGGGCCGATGCCAACGGCGAACTGGGCCCGATCTACGGCTACCAGTGGCGCGCATGGCCGACACCGGACGGTCG
CCATATCGACCAGATCACCGAGGTCGTGCAGCAGATCCGCGACAACCCCGATTCGCGCCGGCTGATTGTGTCGGCCTGGA
ACGTGGGCGAGATCCCGCAGATGAAGCTGCCGCCCTGCCACGCGTTCTTCCAGTTCTACGTGGCCGATGGCAAGCTGTCC
TGCCAGCTCTACCAGCGCAGCGCCGACATTTTCCTCGGCGTGCCGTTCAACATCGCCAGCTACGCGCTGCTGACGCACAT
GATCGCGCAGCAATGCGACCTGGGCGTGGGCGATTTCGTGTGGACCGGTGGCGACTGCCACATCTACAGCAACCACTTCG
AGCAGGTGGAGACGCAGCTTTCGCGCGAGCCGATGAAGCTGCCAACGCTGCGAATCAAGCATCGTCCCGACAGCATCTTC
GACTACAAGTACGACGATTTCGAGCTGGTCGGCTATGAGTCGCACCCCGCGATCAAGGCGCCGGTGGCCGTATGA

Upstream 100 bases:

>100_bases
CGTCTGACGCAGAAAGCCGCCTTCGTGCGGCTTTCTGCTTTCAGGCTAAAATCCCCGCTTGCGGCCCGCACGCCGACGTC
ACTTCCCACTTCCTGTTCCG

Downstream 100 bases:

>100_bases
CGCTGCTGACACTGATCGTCGCGCGCGCGCGCAACGGCGTGATCGGCCGTAACAACACCTTGCCGTGGCGCCTGCCGGAA
GACTTGCAGCATTTCAAGCG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELLWFLQGSTNIKYLKDNGVTIW
DEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIRDNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIF
DYKYDDFELVGYESHPAIKAPVAV

Sequences:

>Translated_264_residues
MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELLWFLQGSTNIKYLKDNGVTIW
DEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIRDNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIF
DYKYDDFELVGYESHPAIKAPVAV
>Mature_264_residues
MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELLWFLQGSTNIKYLKDNGVTIW
DEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIRDNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIF
DYKYDDFELVGYESHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=49.645390070922, Blast_Score=280, Evalue=9e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=73.4848484848485, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=45.9363957597173, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=42.9824561403509, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=50.1766784452297, Blast_Score=280, Evalue=6e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_RALME (Q1LK81)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584714.1
- ProteinModelPortal:   Q1LK81
- SMR:   Q1LK81
- STRING:   Q1LK81
- GeneID:   4039393
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2568
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   ADIPRMK
- PhylomeDB:   Q1LK81
- ProtClustDB:   PRK01827
- BioCyc:   RMET266264:RMET_2568-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30509; Mature: 30509

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGSTNIKYLKDNGVTIWDEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
DNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIF
HHHHHHHHHHHCCCCCCCEEEECCCEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
DYKYDDFELVGYESHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGSTNIKYLKDNGVTIWDEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
DNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIF
HHHHHHHHHHHCCCCCCCEEEECCCEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
DYKYDDFELVGYESHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA