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The map label for this gene is folA [H]

Identifier: 94311505

GI number: 94311505

Start: 2794444

End: 2794944

Strand: Direct

Name: folA [H]

Synonym: Rmet_2569

Alternate gene names: 94311505

Gene position: 2794444-2794944 (Clockwise)

Preceding gene: 94311504

Following gene: 94311507

Centisome position: 71.14

GC content: 63.27

Gene sequence:

>501_bases
ATGACGCTGCTGACACTGATCGTCGCGCGCGCGCGCAACGGCGTGATCGGCCGTAACAACACCTTGCCGTGGCGCCTGCC
GGAAGACTTGCAGCATTTCAAGCGCACGACCATGGGCGCGCCGATCATCATGGGTCGCAAGACCTGGGATTCGATCGGCC
GGCCGCTGCCGGGGCGCCGCAATATCGTGGTGACCCGCAACCGCGACCTGCATATCGAGGGCGCCGATGTGGTGGGATCG
ATCGAGGATGCGCAACGGCTGTGCATAGGTGCGGAACAGGTATTCCTGATCGGCGGCGCACAACTCTATGCGGAGGCACT
GCCGAGCGCCGATCGCCTGATCGTCACGGAAATCGACGCGGATGTGGAAGGCGATGCGTTCTTCCCGGCCGTGGACCGCA
CGACATGGGTCGAGACCGCGCGTGAGCTCCACCATTCGGAAACGAATGGCTTCGACTATGCGTTCGTGACGTACGAACGG
CCGGCTTCCGGGGAAGAATAA

Upstream 100 bases:

>100_bases
GAATCAAGCATCGTCCCGACAGCATCTTCGACTACAAGTACGACGATTTCGAGCTGGTCGGCTATGAGTCGCACCCCGCG
ATCAAGGCGCCGGTGGCCGT

Downstream 100 bases:

>100_bases
AAAAGGGCGGCGCAAACGGGTTCACCCGCTTGCGCCGCCCTTCGTCGTCCCTGATCGGCCCTTGTCGTCTCTCCCGTTTG
GAAGAGACGCACAAACCATC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVTRNRDLHIEGADVVGS
IEDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDADVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYER
PASGEE

Sequences:

>Translated_166_residues
MTLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVTRNRDLHIEGADVVGS
IEDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDADVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYER
PASGEE
>Mature_165_residues
TLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVTRNRDLHIEGADVVGSI
EDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDADVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYERP
ASGEE

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=185, Percent_Identity=34.5945945945946, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI28827793, Length=155, Percent_Identity=34.8387096774194, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1786233, Length=163, Percent_Identity=41.7177914110429, Blast_Score=133, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=186, Percent_Identity=32.7956989247312, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=124, Percent_Identity=31.4516129032258, Blast_Score=64, Evalue=8e-12,
Organism=Drosophila melanogaster, GI116008026, Length=181, Percent_Identity=31.4917127071823, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647458, Length=181, Percent_Identity=31.4917127071823, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18486; Mature: 18355

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRR
CHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCC
NIVVTRNRDLHIEGADVVGSIEDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDA
CEEEECCCEEEEECCHHCCCHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEEEEECC
DVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYERPASGEE
CCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
TLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCC
NIVVTRNRDLHIEGADVVGSIEDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDA
CEEEECCCEEEEECCHHCCCHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEEEEECC
DVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYERPASGEE
CCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]