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The map label for this gene is hpaIM [H]

Identifier: 94311402

GI number: 94311402

Start: 2677216

End: 2678151

Strand: Reverse

Name: hpaIM [H]

Synonym: Rmet_2466

Alternate gene names: 94311402

Gene position: 2678151-2677216 (Counterclockwise)

Preceding gene: 94311403

Following gene: 94311401

Centisome position: 68.18

GC content: 64.96

Gene sequence:

>936_bases
ATGCGTACGCTGCCTCCCAATACGACGCTGCCCCAGTCCGAGGCGGGCGGGGCGGGCTCGGAACCGTTCGATATTCTCGA
TACGGCAACCATGCGCCTCTATCAGGAAGACGTGCTCGAAGGGATCAAGCGTATCCCCGACGGCTCGATCGACCTGGTCG
TGGCGGACCCGCCCTATGGCCTTGGCAAGGACTACGGCAACGATTCCGACCTGCTGTCGGGCGATGCCTATCTCGAGTGG
TCGGAGCGCTGGATGGACGCGATCGTGCCCAAGATCGCGCCACGCGGCACGCTGTACCTGTTCTGCACCTGGCAGTACTC
GCCCGAGCTGTTCGTCATGCTCAAGCGGCGCATGACGATGATCAACGAGATCATCTGGGATCGCCGCGTGCCGAGCATGG
GCGGCACCACGCGAAAGTTCTCGTCGGTGCACGACAACATCGGTTTCTTCGCTCGTCAGCGCGACTACTTCTTCGATCTC
GATCCGGTACGGATTCCGTACGACGCCGAGACCAAGAAGGCGCGCAGCCGGCCGCGCTTCGAGGGCAAGAAATGGCTCGA
GGTGGGGTACAACCCGAAGGACCTGTGGAGCGTGCCGCGCATTCACCGCCAGGACCCGGAGCGGGCCGACCATCCGACGC
AGAAGCCGCTCGAGATCGTCGAGCGCATGGTGCTGTCCAGCTGTCCGCCGGGCGGCATCGTGCTCGACCCATTCACGGGC
AGCGGCACGACCGCCGTGGCTTGCGTCCGGCACGGCCGCAGTTTCGTCGGATTCGAGATGAATCCGGAGTACGCCGGCCT
GGTGCGCGAGCGCGTGACCGCCGCGCAGCCCGTGTATGCGCCGCTCAAGCCGCAAGCGTCCGATGTCGCCGATACCGCCG
ACGCCGCCAATGACGATGCCAATGAAGCGGCATCGCCCGTGCAACGCCTTATCTGA

Upstream 100 bases:

>100_bases
CGCCGACGCTGGGCCGCGACAAGCTGCTGCTCGTGAACCTGTCCGGGCGTGGCGACAAGGACATGCATACCGTGGCCGAA
CGCGCGGGGTTGAAGCTCTG

Downstream 100 bases:

>100_bases
TTGCCACTGACATGTCCCGTATCCAGAAGACTTTCGCGGCACTGGCCGCGCAGCAGAAGAAGGGCCTGATTCCGTTCATC
ACCGCCGGCGATCCGGCTCC

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYGLGKDYGNDSDLLSGDAYLEW
SERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTMINEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDL
DPVRIPYDAETKKARSRPRFEGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTG
SGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDANEAASPVQRLI

Sequences:

>Translated_311_residues
MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYGLGKDYGNDSDLLSGDAYLEW
SERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTMINEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDL
DPVRIPYDAETKKARSRPRFEGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTG
SGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDANEAASPVQRLI
>Mature_311_residues
MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYGLGKDYGNDSDLLSGDAYLEW
SERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTMINEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDL
DPVRIPYDAETKKARSRPRFEGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTG
SGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDANEAASPVQRLI

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=263, Percent_Identity=29.277566539924, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 34883; Mature: 34883

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
LGKDYGNDSDLLSGDAYLEWSERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTM
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
INEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDLDPVRIPYDAETKKARSRPRF
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCEECCCCEECCCCCHHHHHHCCCCC
EGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTG
CCCHHEECCCCHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCC
SGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDA
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCH
NEAASPVQRLI
HHHHHHHHHHC
>Mature Secondary Structure
MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
LGKDYGNDSDLLSGDAYLEWSERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTM
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
INEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDLDPVRIPYDAETKKARSRPRF
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCEECCCCEECCCCCHHHHHHCCCCC
EGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTG
CCCHHEECCCCHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCC
SGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDA
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCH
NEAASPVQRLI
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]