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The map label for this gene is purF [H]

Identifier: 94311396

GI number: 94311396

Start: 2670812

End: 2672344

Strand: Reverse

Name: purF [H]

Synonym: Rmet_2460

Alternate gene names: 94311396

Gene position: 2672344-2670812 (Counterclockwise)

Preceding gene: 94311397

Following gene: 94311395

Centisome position: 68.03

GC content: 62.95

Gene sequence:

>1533_bases
ATGTGCGGTATCGTCGGTGCGGTTTCCACCAGCCCTGTCAATCAACTCATCTATGACAGCCTGCTGCTGTTGCAACACCG
CGGACAGGATGCAGCCGGCATCGCCACGGCCAATGGCAGCACCTTTCACATGCACAAGGCCAACGGCATGGTGCGTGACG
TGTTCCGCACGCGCAACATGCGCAGCCTGCCGGGCACGTCGGGCATCGGCCAGGTGCGCTACCCGACCGCCGGTTCCGCG
TCGAGCGAGGAAGAAGCACAGCCGTTCTATGTGAACGCGCCGTACGGCGTCATCCTCGCCCACAACGGCAACCTGACCAA
CTCGGAACAGTTGCGCGAGGAAATGTTCCGTCGCGATCGCCGCCACATCAACACGCATTCGGACTCCGAAGTGCTGCTGA
ACGTGCTGGCCGACGAACTGCAACGCGCCAGCAGCGGCAACGAACTGGACCCGGAGACAATCTTCAAGGCCGTCGCGGGC
ATGCACCGCCGCGTCAAGGGTGCCTATGCGATCACCGCGCAGATCGCCGGCTACGGCCTGCTGGCCGTACGCGATCCGTT
CGGCATCCGCCCGCTGTGTATTGGCAGCGTCGACACCCCGACCGGCAAGGAATACCTCATCGCCTCGGAATCCGTGGCCC
TGGAAGGCATCGGCTACCAGATGGAGCGCGATGTGGCGCCGGGCGAGGCGATTTTCATCGACCTGGACGGCTCGTTCCAC
AGCAAGCAGTGCGCTGATAATCCGGTGCTGACGCCATGCATCTTCGAGTATGTCTATCTGGCCCGTCCGGATTCGTGCAT
TGACGGCGTGCCGGTCTACGATGCCCGCCTGCGCATGGGCGACTACCTGGCCGAAAAGATCCGCCGCGAAGTGCCGGCTG
GCGATATTGACGTGGTCATGCCGATTCCCGATTCGAGCCGCCCGGCCGCCATGCAGGTGGCCAATGCGCTCGGCGTGCAG
TACCGCGAGGGCTTCTTCAAGAACCGCTACGTGGGCCGTACGTTCATCATGCCGGGCCAGGCAGTGCGCAAGAAGTCGGT
GCGCCAGAAGCTCAATGCGATGGGTGTCGAGTTCAAGGGCAAGAACGTGCTGATCGTCGACGATTCGATCGTCCGTGGTA
CCACGTCGCTCGAGATCGTGCAGATGGCGCGTGACGCAGGTGCGAAGAAGGTGATCTTCGCGTCGGCAGCGCCGCCGGTG
AAGTTCCCGAACGTCTACGGTATCGACATGCCCACGCGTGCCGAACTGGTGGCCTATGGTCGCACGGACGAAGAAGTGGC
GCGCATGATCGGCGCCGACCAGCTCGTCTACCAGGACGTGGAAGCGATGAAGCAGGCTGTGCGCGACATCAACCCGAACC
TGCGTGACTTTGATGCGTCGTGCTTCGACGGCAAGTACGTTACCGGCGATATCGACGACGCCTATCTGGAGCGCCTCGAG
ACCGCGCGTAGCCTGGCCGATCGTGAAGGCACCAACGGCGGTGAAACCGAACGTTCGCAGCTGCATCTGCAGCGCTCGGG
CGGCAACGAATAA

Upstream 100 bases:

>100_bases
GCTCGACAGAGCGCACCCGCGGCGACAGTGACAACGCAGATCCCGTTCTCCCTTGACGGGATTGCGCCCCTGAATCATTT
GCCTTTCCTGGGAGTTCGGC

Downstream 100 bases:

>100_bases
CAAGAGACAAGAATCTTCGGAAGGCAGCAAAGCAGCATGAGCGAACCGTTCAACCCCGACGCCTACGGCATTGATACCCT
CGGCGTGCGCGCCGGCACGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGVILAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASSGNELDPETIFKAVAG
MHRRVKGAYAITAQIAGYGLLAVRDPFGIRPLCIGSVDTPTGKEYLIASESVALEGIGYQMERDVAPGEAIFIDLDGSFH
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRREVPAGDIDVVMPIPDSSRPAAMQVANALGVQ
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMGVEFKGKNVLIVDDSIVRGTTSLEIVQMARDAGAKKVIFASAAPPV
KFPNVYGIDMPTRAELVAYGRTDEEVARMIGADQLVYQDVEAMKQAVRDINPNLRDFDASCFDGKYVTGDIDDAYLERLE
TARSLADREGTNGGETERSQLHLQRSGGNE

Sequences:

>Translated_510_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGVILAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASSGNELDPETIFKAVAG
MHRRVKGAYAITAQIAGYGLLAVRDPFGIRPLCIGSVDTPTGKEYLIASESVALEGIGYQMERDVAPGEAIFIDLDGSFH
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRREVPAGDIDVVMPIPDSSRPAAMQVANALGVQ
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMGVEFKGKNVLIVDDSIVRGTTSLEIVQMARDAGAKKVIFASAAPPV
KFPNVYGIDMPTRAELVAYGRTDEEVARMIGADQLVYQDVEAMKQAVRDINPNLRDFDASCFDGKYVTGDIDDAYLERLE
TARSLADREGTNGGETERSQLHLQRSGGNE
>Mature_510_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGVILAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASSGNELDPETIFKAVAG
MHRRVKGAYAITAQIAGYGLLAVRDPFGIRPLCIGSVDTPTGKEYLIASESVALEGIGYQMERDVAPGEAIFIDLDGSFH
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRREVPAGDIDVVMPIPDSSRPAAMQVANALGVQ
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMGVEFKGKNVLIVDDSIVRGTTSLEIVQMARDAGAKKVIFASAAPPV
KFPNVYGIDMPTRAELVAYGRTDEEVARMIGADQLVYQDVEAMKQAVRDINPNLRDFDASCFDGKYVTGDIDDAYLERLE
TARSLADREGTNGGETERSQLHLQRSGGNE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=479, Percent_Identity=37.3695198329854, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1788651, Length=504, Percent_Identity=56.9444444444444, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1790167, Length=218, Percent_Identity=29.8165137614679, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=34.7107438016529, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=50.10183299389, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28573187, Length=503, Percent_Identity=35.7852882703777, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=36.3453815261044, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=37.0292887029289, Blast_Score=273, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55868; Mature: 55868

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGMVRDVFRTRNM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RSLPGTSGIGQVRYPTAGSASSEEEAQPFYVNAPYGVILAHNGNLTNSEQLREEMFRRDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTHSDSEVLLNVLADELQRASSGNELDPETIFKAVAGMHRRVKGAYAITAQIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
LAVRDPFGIRPLCIGSVDTPTGKEYLIASESVALEGIGYQMERDVAPGEAIFIDLDGSFH
EEEECCCCCCEEEECCCCCCCCCEEEEECCCEEEECCCCCHHCCCCCCCEEEEECCCCCC
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRREVPAGDIDVVM
CCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVANALGVQYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMGVEFKG
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEECC
KNVLIVDDSIVRGTTSLEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRAELVAYG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCCCCCCEEEEC
RTDEEVARMIGADQLVYQDVEAMKQAVRDINPNLRDFDASCFDGKYVTGDIDDAYLERLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHHH
TARSLADREGTNGGETERSQLHLQRSGGNE
HHHHHHHCCCCCCCCCCHHHEEEECCCCCC
>Mature Secondary Structure
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGMVRDVFRTRNM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RSLPGTSGIGQVRYPTAGSASSEEEAQPFYVNAPYGVILAHNGNLTNSEQLREEMFRRDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTHSDSEVLLNVLADELQRASSGNELDPETIFKAVAGMHRRVKGAYAITAQIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
LAVRDPFGIRPLCIGSVDTPTGKEYLIASESVALEGIGYQMERDVAPGEAIFIDLDGSFH
EEEECCCCCCEEEECCCCCCCCCEEEEECCCEEEECCCCCHHCCCCCCCEEEEECCCCCC
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRREVPAGDIDVVM
CCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVANALGVQYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMGVEFKG
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEECC
KNVLIVDDSIVRGTTSLEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRAELVAYG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCCCCCCEEEEC
RTDEEVARMIGADQLVYQDVEAMKQAVRDINPNLRDFDASCFDGKYVTGDIDDAYLERLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHHH
TARSLADREGTNGGETERSQLHLQRSGGNE
HHHHHHHCCCCCCCCCCHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]