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The map label for this gene is metB [H]

Identifier: 94311395

GI number: 94311395

Start: 2669567

End: 2670775

Strand: Reverse

Name: metB [H]

Synonym: Rmet_2459

Alternate gene names: 94311395

Gene position: 2670775-2669567 (Counterclockwise)

Preceding gene: 94311396

Following gene: 94311392

Centisome position: 67.99

GC content: 64.52

Gene sequence:

>1209_bases
ATGAGCGAACCGTTCAACCCCGACGCCTACGGCATTGATACCCTCGGCGTGCGCGCCGGCACGCTGCGCACTGGCGAATT
CATGGAGCACTCCGAGGCGATGTACCTGACCTCGAGCTTCTGCTTCAACAGTGCAGCCGAAGCCGCCGCACGCTTCGCCA
ACTCGGAAGAGGGTTACACCTACTCGCGCTTCACGAATCCGACCGTGTCGATGTTCCAGTCGCGCCTGGCCGCGCTGGAA
GGCGCGGAGGCCTGCATGGCCACGGCTTCGGGCATGAGCGCGATTCTGTCGGTGGCGCTCGCCACGCTGCAGGCCGGCGA
TCACCTGGTCAGCTCGCGTTCGATCTTCGGCTCGACGATGACGCTGTTCAACTCGATCCTGGCCAAGTTCGGTGTGGAGA
CGACCTACGTCGACGGCACCGATCTGGCTGCCTGGCGTGCCGCTGTGAAGCCGAACACGAAGCTGTTCTTCCTGGAGACG
CCGTCGAACCCGCTGACCGAGGTGTCCGATATCGCGGCAGTTGCCGATATTGCGCACAATGCCGGCGCGCTGTTCGTGGT
CGACAACTGCTTCTGCTCACCGGCTTTGCAGCAGCCGATCAAGTTCGGTGCCGATGTGGTGGTGCATTCGGCCACCAAGC
ACATCGATGGCCAGGGCCGCGTGCTTGGCGGCGCGGTGGTCGGCAAGCACGATTTCATCATGGGCAAGGTGTTCCCGTTC
GTGCGTACGGCGGGCCCGACGCTGTCGGCGTTCAACGCGTGGGTGATGCTCAAGGGCATGGAAACGCTGGCGATCCGCAT
GGAGCGTCACTCGCAGAGCGCGTTGGCGATTGCCGAGTTCCTCGAGTCACATCCGGCCGTGAATCGTGTGTTCCACCCGG
CGCTGAAGTCGCATCCGCAGTACGAGATCGCCCAACGCCAGCAGAGCGGGGGCGGCGCGATCGTGTCGTTCGAGTTGAAG
GGCGATAGCCCCGAAGCCATGCGTGCTGCTGCGTGGCGCGTGATCGACAGCACGAAGCTGTGCTCGATCACCGGCAATCT
CGGCGACACGCGCACGACGATCACCCATCCGTACACCACCACCCACGGTCGCGTGGCGCCTGAAGCCAAGGCCGCCGCCG
GCATCAGCGAAGGGCTGATCCGACTGGCCGTTGGCCTGGAGTCCGTGGAGGATCTCAAGGCCGATCTGCTGCGCGGCCTG
GGCCAGTAA

Upstream 100 bases:

>100_bases
CACCAACGGCGGTGAAACCGAACGTTCGCAGCTGCATCTGCAGCGCTCGGGCGGCAACGAATAACAAGAGACAAGAATCT
TCGGAAGGCAGCAAAGCAGC

Downstream 100 bases:

>100_bases
GCGGTTGGTTTTTCTCCCCTTGCCCATGACTGGGCGAGGGGGGCTTCACCGCAGCATCCTCTTCCTCAGCAGCACCAGCG
CCACCGTCAGCGCCACGACT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYTYSRFTNPTVSMFQSRLAALE
GAEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTMTLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLET
PSNPLTEVSDIAAVADIAHNAGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPF
VRTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQYEIAQRQQSGGGAIVSFELK
GDSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTTTHGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGL
GQ

Sequences:

>Translated_402_residues
MSEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYTYSRFTNPTVSMFQSRLAALE
GAEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTMTLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLET
PSNPLTEVSDIAAVADIAHNAGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPF
VRTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQYEIAQRQQSGGGAIVSFELK
GDSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTTTHGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGL
GQ
>Mature_401_residues
SEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYTYSRFTNPTVSMFQSRLAALEG
AEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTMTLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLETP
SNPLTEVSDIAAVADIAHNAGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPFV
RTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQYEIAQRQQSGGGAIVSFELKG
DSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTTTHGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGLG
Q

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=329, Percent_Identity=35.5623100303951, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI299473759, Length=329, Percent_Identity=32.5227963525836, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI25453487, Length=328, Percent_Identity=29.2682926829268, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=38.860103626943, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1789383, Length=402, Percent_Identity=28.6069651741293, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=334, Percent_Identity=39.5209580838323, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=38.855421686747, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.0540540540541, Blast_Score=209, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=428, Percent_Identity=32.9439252336449, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=361, Percent_Identity=37.3961218836565, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321254, Length=420, Percent_Identity=26.9047619047619, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=294, Percent_Identity=29.2517006802721, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=359, Percent_Identity=25.3481894150418, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922554, Length=346, Percent_Identity=35.2601156069364, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42801; Mature: 42670

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
YSRFTNPTVSMFQSRLAALEGAEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTM
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLETPSNPLTEVSDIAAVADIAHN
HHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
AGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPF
CCEEEEEECCCCCHHHHHHHHCCCCEEEEHHHHCCCCCCEEEECEEECCCHHHHHHHHHH
VRTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQ
HHCCCCCHHHHHHEEEEECHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
YEIAQRQQSGGGAIVSFELKGDSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTT
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCEEC
THGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGLGQ
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
YSRFTNPTVSMFQSRLAALEGAEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTM
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLETPSNPLTEVSDIAAVADIAHN
HHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
AGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPF
CCEEEEEECCCCCHHHHHHHHCCCCEEEEHHHHCCCCCCEEEECEEECCCHHHHHHHHHH
VRTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQ
HHCCCCCHHHHHHEEEEECHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
YEIAQRQQSGGGAIVSFELKGDSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTT
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCEEC
THGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGLGQ
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]