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The map label for this gene is rhtB [H]

Identifier: 94311392

GI number: 94311392

Start: 2666967

End: 2667584

Strand: Reverse

Name: rhtB [H]

Synonym: Rmet_2456

Alternate gene names: 94311392

Gene position: 2667584-2666967 (Counterclockwise)

Preceding gene: 94311395

Following gene: 94311386

Centisome position: 67.91

GC content: 64.89

Gene sequence:

>618_bases
ATGACTTTCGAACACTGGATTACGTTCGTGTTGACCAGCGTGATCCTGCTGATCATTCCAGGGCCGACGATCCTCCTTGT
GATCGGCGATGCGCTCGCGCACCGCGGCCGCAGCGCCTATGCCACGGTGGCTGGTGTGGCTTGTGGCGACCTGACGGCCT
TCACGCTGTCGATGGCCGGCCTGGGAGCGGTGCTGGCGGCCTCTGCCACGCTTTTCACGGCCCTCAAGTGGGTTGGGGCG
GCCTATCTGGTCTATCTCGGTGTGCAGGCGCTGCGTACCGCGAATCAGGCCCGGGAACTCGAGGTGGCGCCGCAGTCCGG
CAGCGGCGCCCAGCGCTTTGCCAAGGCATGGTTGGTCACGGCGCTGAACCCGAAGAGCATCGTGTTCTTCGTGGCCTTTG
TGCCGCAGTTCATGGATGCGAAGTTGGCATTCTGGTCGCAGGCCGTGGTGCTGATCCCGACCTTCGTTGTGCTGGCGAGT
GCCAATGCCACGGTTTATGCGCTGCTGGCGCGTTCGCTGGCGACTCGGCTGACCACGCGACGGGCGCAGGCCAATATGCA
GCGTGCCGGCGGCATTACGCTGATTGGGGCGGGCGCGCTGATCGCCACGCGTTCCTAG

Upstream 100 bases:

>100_bases
TCACGGCGAGACAATTCCATTTGCCGGAGTGTAGGTTGGCTAACGCGCGACGTTCGGCTTACTCTTAATATTTCCGAAGC
CGGACTTCAAAGGAGCCGTG

Downstream 100 bases:

>100_bases
GCGGTGCCGCAGGGACGAAAAAAAACCGCCAGCTTGCTGGCGGTTTTTCTTGGCAATGCGCGAATCAGTAGAGCTTCTTC
GGCAGCATTTGCATACGCAG

Product: LysE family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAGLGAVLAASATLFTALKWVGA
AYLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVTALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLAS
ANATVYALLARSLATRLTTRRAQANMQRAGGITLIGAGALIATRS

Sequences:

>Translated_205_residues
MTFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAGLGAVLAASATLFTALKWVGA
AYLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVTALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLAS
ANATVYALLARSLATRLTTRRAQANMQRAGGITLIGAGALIATRS
>Mature_204_residues
TFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAGLGAVLAASATLFTALKWVGAA
YLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVTALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLASA
NATVYALLARSLATRLTTRRAQANMQRAGGITLIGAGALIATRS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=203, Percent_Identity=34.4827586206897, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788099, Length=138, Percent_Identity=27.536231884058, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21554; Mature: 21422

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAG
CCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAVLAASATLFTALKWVGAAYLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
ALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLASANATVYALLARSLATRLTTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAQANMQRAGGITLIGAGALIATRS
HHHHHHHHCCCEEEEECCEEEEECC
>Mature Secondary Structure 
TFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAG
CHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAVLAASATLFTALKWVGAAYLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
ALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLASANATVYALLARSLATRLTTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAQANMQRAGGITLIGAGALIATRS
HHHHHHHHCCCEEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]