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The map label for this gene is ccmE

Identifier: 94311325

GI number: 94311325

Start: 2599831

End: 2600277

Strand: Direct

Name: ccmE

Synonym: Rmet_2389

Alternate gene names: 94311325

Gene position: 2599831-2600277 (Clockwise)

Preceding gene: 94311324

Following gene: 94311326

Centisome position: 66.19

GC content: 60.63

Gene sequence:

>447_bases
GTGAAGCCACGAAATAGACGCATCGCACTCATCGTCGCCGGGCTATCCGCCCTCGGCATCGCCACCGCGCTGGTGCTCAA
TGCCTTTCAGAGCAACCTTGTGTTCTTCTTCACGCCCTCGCAGGTCAGTGCCGGGGAGGCACCACTGGAGCGCACCTTTC
GAGTCGGCGGCATGGTGGAACGAGGTAGTCTGAAGCGCCAGCGTGGTGAGCTTGCCGTGCAGTTCGTCATCACCGATACG
GTCAAAGCAATCCCGGTCACGTATTCGGGCATCTTGCCTGACCTGTTCAGTGAAGGAAAAGGGGTGGTTGTTCAAGGACG
GCTGGACAGTGCCGGCCTCTTTCGCGCCGAGGAGGTGCTGGCCAAGCACGACGAGAACTACATGCCGCCCGAGGCCCAGC
ATGCGCTGGACGAAGTCCAGAAGAAGCCGGCTTCAAGGAAGCCTTGA

Upstream 100 bases:

>100_bases
ACGGTGGTGTTGCTCTGGACTGAATGCCGAATGCTCCGGCGCCGGCGCCGAGCAGCGCTATGGCGCATCCAGTCGGAATT
GCTGGGCAAGGAGGCGCGGC

Downstream 100 bases:

>100_bases
CCCATGATTCCAGAAATCGGCCACCTCGCCCTCATCGCTGCCTTGTTCGTGGCACTCGCTCAAGGTGTTCTTGCCCTGGC
CGGCGCGGCGCGAGCCAACC

Product: periplasmic heme chaperone

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKPRNRRIALIVAGLSALGIATALVLNAFQSNLVFFFTPSQVSAGEAPLERTFRVGGMVERGSLKRQRGELAVQFVITDT
VKAIPVTYSGILPDLFSEGKGVVVQGRLDSAGLFRAEEVLAKHDENYMPPEAQHALDEVQKKPASRKP

Sequences:

>Translated_148_residues
MKPRNRRIALIVAGLSALGIATALVLNAFQSNLVFFFTPSQVSAGEAPLERTFRVGGMVERGSLKRQRGELAVQFVITDT
VKAIPVTYSGILPDLFSEGKGVVVQGRLDSAGLFRAEEVLAKHDENYMPPEAQHALDEVQKKPASRKP
>Mature_148_residues
MKPRNRRIALIVAGLSALGIATALVLNAFQSNLVFFFTPSQVSAGEAPLERTFRVGGMVERGSLKRQRGELAVQFVITDT
VKAIPVTYSGILPDLFSEGKGVVVQGRLDSAGLFRAEEVLAKHDENYMPPEAQHALDEVQKKPASRKP

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=148, Percent_Identity=43.9189189189189, Blast_Score=127, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME2_PSEAE (Q8GQ96)

Other databases:

- EMBL:   AF440523
- ProteinModelPortal:   Q8GQ96
- SMR:   Q8GQ96
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 16103; Mature: 16103

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: BINDING 123-123

Signals:

None

Transmembrane regions:

HASH(0x1620e94c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRNRRIALIVAGLSALGIATALVLNAFQSNLVFFFTPSQVSAGEAPLERTFRVGGMVE
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHCCEEC
RGSLKRQRGELAVQFVITDTVKAIPVTYSGILPDLFSEGKGVVVQGRLDSAGLFRAEEVL
CCCHHHHCCCEEEEEEEECCCCCCCEECCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHH
AKHDENYMPPEAQHALDEVQKKPASRKP
HHCCCCCCCCHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MKPRNRRIALIVAGLSALGIATALVLNAFQSNLVFFFTPSQVSAGEAPLERTFRVGGMVE
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHCCEEC
RGSLKRQRGELAVQFVITDTVKAIPVTYSGILPDLFSEGKGVVVQGRLDSAGLFRAEEVL
CCCHHHHCCCEEEEEEEECCCCCCCEECCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHH
AKHDENYMPPEAQHALDEVQKKPASRKP
HHCCCCCCCCHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12426355