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The map label for this gene is glpK [H]

Identifier: 94311176

GI number: 94311176

Start: 2453323

End: 2454855

Strand: Direct

Name: glpK [H]

Synonym: Rmet_2238

Alternate gene names: 94311176

Gene position: 2453323-2454855 (Clockwise)

Preceding gene: 94311173

Following gene: 94311177

Centisome position: 62.46

GC content: 66.73

Gene sequence:

>1533_bases
ATGACATCCACGACATCTCATCAGCCGCAGTACGTGCTGGCCCTGGATCAGGGCACCAGCAGTTCGCGCGCCATCCTGTT
CGATCACCTGGGCAATGTCGTACGCATCGCCCAGCGTGAGTTTCGGCAATATTATCCGCATCCCGGCCACGTCGAGCACG
ACCCCTATGAGATCTGGCAGTCGCAACTGGCCGTGGCGCACGAGGTACTGGGCGACGCGGGCGTCAGCGGCACGCAGATT
TCTGCCATCGGCATCACGAACCAGCGCGAAACCACGGTGCTGTGGGATCGCAAGACCGGTGAACCGGTGGGCCGCGCGCT
GGTCTGGCAAGACCGCCGCACCGCACCGATGTGCGAGGACCTGCAGGCAGCGGGCCACGCGGAGACATTTCAGCGCAAGA
CCGGACTGATCGTCGACGCCTATTTCTCCGGCACCAAACTGCGCTGGATGCTCGACAACTACGAGGGCGCCCGCGCACGC
GCCGAGCGCGGCGAGTTGGCCTTTGGCACCGTCGATAGCTGGCTGATCTGGCAGTTGACCGATGGTGCGCGGCACGTCAC
CGACGTTTCCAATGCTTCGCGCACGATGCTGTTCAACATCCACGATTTCCGCTGGGACGATGAACTCCTGGGCCTGCTGG
ATATTCCGCGCAACCTGCTGCCGGAAGTGGTGCCGTCGAGCGGCGAGGTCGCGCGCGCATCGGCACGGCTGTTCGGCGTG
GAGATTCCGATTGCCGGCATCGCCGGCGACCAGCAGGCCGCCACGTTTGGCCAAGCTTGCCTGCGGCCGGGCATGGCCAA
GAACACCTATGGCACCGGCTGCTTCATCCTGATGAACACCGGCGACAAACCCGTAACATCGCACAACCGGCTGATCACGA
CCATCGGCTGGCAGATCGGCGGCAATACCCAGTACTGTCTGGAAGGCGGCGTATTCATGGGCGGCGCGACGATCCAGTGG
CTGCGCGACGGCCTCAAGCTGATCCACAGCGCCCCCGAGGTAGAGCCTCTGGCCCGCCAGTGCACCGACACCGGCGGCGT
GGTGCTGGTGCCGGCATTCGCCGGCCTCGGCGCGCCGCACTGGGATCCGTTCGCACGCGGCACGTTGGTCGGCATCACGC
GCGGTACCGGCGGGCCGCACATCGCCCGGGCCGCGCTCGAATCCATCGCGCTGCAGAGCGTGGATGTGCTCGACGCCATG
CAGAAGGACGCCGGCATCTCGCTGGCCGAACTCCGGGTCGATGGCGGTGCCTCGCGCAGCGACCTGCTGATGCAGATGCA
GGCCGACCTGCTCGGCACCGTCGTGGTCCGGCCGCGCGTGACCGAAACCACCGCGCTAGGCGCGGCATATCTCGCCGGGC
TGGCAACTGGTTACTGGACCGACCCCGAAGAAATCACGCGGCAGTGGCAGGTGGAGCAGCGCTTCGAGCCGAAGCTGTCC
GCCGACGAACGCGAACGCCGCATTGCGCGTTGGCACCGCGCCGTCGACCGCGCCCGCGACTGGGCGCGCGAGTCCGATGA
CGCCGCACGCTGA

Upstream 100 bases:

>100_bases
CAACGCCAGCAGTGACTACGCTAACGATATCGGCGGGATCGGCGGGGCGGGAAACTGTTTCGGCCATGGCTATTCAATTC
AACACATCCGGATGGAACGC

Downstream 100 bases:

>100_bases
ACCCAGATTCGAATTTCCCATGCAAACTTCCGTCACTCCGATCGCCCCTCCCTCCCGCGACACCCTGCTTCAGACGCTCG
CGCGCGAGCCACGCTGGGAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQSQLAVAHEVLGDAGVSGTQI
SAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEDLQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARAR
AERGELAFGTVDSWLIWQLTDGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGV
EIPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIGGNTQYCLEGGVFMGGATIQW
LRDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAM
QKDAGISLAELRVDGGASRSDLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLS
ADERERRIARWHRAVDRARDWARESDDAAR

Sequences:

>Translated_510_residues
MTSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQSQLAVAHEVLGDAGVSGTQI
SAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEDLQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARAR
AERGELAFGTVDSWLIWQLTDGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGV
EIPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIGGNTQYCLEGGVFMGGATIQW
LRDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAM
QKDAGISLAELRVDGGASRSDLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLS
ADERERRIARWHRAVDRARDWARESDDAAR
>Mature_509_residues
TSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQSQLAVAHEVLGDAGVSGTQIS
AIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEDLQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARARA
ERGELAFGTVDSWLIWQLTDGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGVE
IPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIGGNTQYCLEGGVFMGGATIQWL
RDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAMQ
KDAGISLAELRVDGGASRSDLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLSA
DERERRIARWHRAVDRARDWARESDDAAR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=510, Percent_Identity=43.7254901960784, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI4504007, Length=510, Percent_Identity=43.7254901960784, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI42794763, Length=516, Percent_Identity=43.2170542635659, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI41393575, Length=504, Percent_Identity=44.6428571428571, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=34.6978557504873, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=57.1717171717172, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1787789, Length=514, Percent_Identity=26.8482490272374, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790005, Length=518, Percent_Identity=26.6409266409266, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789987, Length=478, Percent_Identity=25.9414225941423, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789138, Length=499, Percent_Identity=24.4488977955912, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789168, Length=489, Percent_Identity=23.9263803680982, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=508, Percent_Identity=40.748031496063, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=26.1538461538462, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=30.7432432432432, Blast_Score=133, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=551, Percent_Identity=36.8421052631579, Blast_Score=309, Evalue=6e-85,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=40.5511811023622, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=436, Percent_Identity=42.6605504587156, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24655555, Length=475, Percent_Identity=39.3684210526316, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24655560, Length=475, Percent_Identity=39.3684210526316, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24655570, Length=475, Percent_Identity=39.3684210526316, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24655574, Length=475, Percent_Identity=39.3684210526316, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24655565, Length=475, Percent_Identity=39.3684210526316, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI19922058, Length=517, Percent_Identity=31.1411992263056, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24652871, Length=499, Percent_Identity=31.4629258517034, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=29.4573643410853, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI21357867, Length=531, Percent_Identity=28.8135593220339, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=31.8051575931232, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=28.2758620689655, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55987; Mature: 55856

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQ
CCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
SQLAVAHEVLGDAGVSGTQISAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCED
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCCCCHHH
LQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARARAERGELAFGTVDSWLIWQLT
HHHCCCHHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHCCCCEEEECCCCEEEEEEC
DGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGV
CCCHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHEEEEE
EIPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIG
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEC
GNTQYCLEGGVFMGGATIQWLRDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPH
CCEEEEEECCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECHHCCCCCC
WDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAMQKDAGISLAELRVDGGASRS
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
DLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLS
HHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
ADERERRIARWHRAVDRARDWARESDDAAR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQ
CCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
SQLAVAHEVLGDAGVSGTQISAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCED
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCCCCHHH
LQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARARAERGELAFGTVDSWLIWQLT
HHHCCCHHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHCCCCEEEECCCCEEEEEEC
DGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGV
CCCHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHEEEEE
EIPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIG
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEC
GNTQYCLEGGVFMGGATIQWLRDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPH
CCEEEEEECCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECHHCCCCCC
WDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAMQKDAGISLAELRVDGGASRS
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
DLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLS
HHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
ADERERRIARWHRAVDRARDWARESDDAAR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA