Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 94311174

GI number: 94311174

Start: 2451524

End: 2452390

Strand: Reverse

Name: purU [H]

Synonym: Rmet_2236

Alternate gene names: 94311174

Gene position: 2452390-2451524 (Counterclockwise)

Preceding gene: 94311175

Following gene: 94311172

Centisome position: 62.43

GC content: 63.32

Gene sequence:

>867_bases
ATGAGTTCCACCGGATTTATCCTCACGCTTTCCTGCCCGGACCAGCCGGGCATCGTTCACGCCGTATCCGGCCTGCTGTT
CCAGCACGGCTGCAATATCGTTGACTCCGACCAGTACGGTGATGCCTTCGCCGGCCGCTTCTTTATGCGCGTGCATTTCA
GCGCCGCGCCGGGCGGCCCGGATCTGGCCGAGCTTCGTGCCGCGTTTGCCCCCGTGGGCGACCAGTTCGGCATGCAGTGG
GAGGTGCACGACGCGACCGTGAAGCCGCGCGTGATGATCATGGTGTCGAAGATTGGCCACTGCCTGAACGACCTGCTGTT
CCGCGCCAAGGTTGGCGGTCTGCCGGTGGAGATCGCGGCGATCGTCTCGAACCATCGTGACTTCTATCAGCTCGCCGCGT
CGTACGACGTGCCGTTCTTCCATCTGCCGCTGATGAACGCATCGGCGGAGCAGAAGGCCGCGCAGGAAGCGCGCGTGTTT
GAAGTCGTGCGCGAGCAGAATATCGACCTGGTCGTGCTGGCCCGCTACATGCAGGTGCTGTCCGATGACCTGTGCCGCAA
GCTCCAGGGCCGCGCGATCAACATCCACCATTCGTTCCTGCCGAGCTTCAAGGGCGCCAAGCCTTACTACCAGGCGCATG
ACCGCGGCGTGAAGCTGATTGGCGCGACGGCCCACTACGTGACTGCCGACCTCGACGAAGGCCCGATCATCGAACAGGAG
ATCGAGCGCGTGGACCACAGCATGGATCCGGAGCAACTGACCGCCGTGGGTCGCGACGTCGAATGCGTGGCGCTGGCCCG
CGCCGTCAAGTGGCATGCGGAACATCGGATTCTGCTGAACGGCCACAAGACGGTGGTGTTCAAGTAA

Upstream 100 bases:

>100_bases
TGGTGACGCTGGATGCCCTCCGCCGACGCGGCTGGGCCGGTATCCAGCCCTGAATCGAGGCCTTCACGGTCCCGGCCCGC
TCAATCCCGAATCGCTTACG

Downstream 100 bases:

>100_bases
GGACCGGAAACCGCAATGGTTTTCTCCCCTCTCCCGAACGGGGGAGAGGGGCGGAGAGAGGGACGGGGAGAAGGGCAGGC
GGCTCAAATTGCGACCTGTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGPDLAELRAAFAPVGDQFGMQW
EVHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVF
EVVREQNIDLVVLARYMQVLSDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Sequences:

>Translated_288_residues
MSSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGPDLAELRAAFAPVGDQFGMQW
EVHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVF
EVVREQNIDLVVLARYMQVLSDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
>Mature_287_residues
SSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGPDLAELRAAFAPVGDQFGMQWE
VHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVFE
VVREQNIDLVVLARYMQVLSDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQEI
ERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=153, Percent_Identity=31.3725490196078, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869995, Length=153, Percent_Identity=31.3725490196078, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869993, Length=153, Percent_Identity=31.3725490196078, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=39.0070921985816, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=25.130890052356, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=27.5510204081633, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31913; Mature: 31782

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGP
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCHHHCEEEEEEEEECCCCCC
DLAELRAAFAPVGDQFGMQWEVHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAA
CHHHHHHHHCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
IVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVFEVVREQNIDLVVLARYMQVL
HHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure 
SSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGP
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCHHHCEEEEEEEEECCCCCC
DLAELRAAFAPVGDQFGMQWEVHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAA
CHHHHHHHHCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
IVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVFEVVREQNIDLVVLARYMQVL
HHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]