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The map label for this gene is nrtB [H]

Identifier: 94311158

GI number: 94311158

Start: 2434056

End: 2435099

Strand: Direct

Name: nrtB [H]

Synonym: Rmet_2220

Alternate gene names: 94311158

Gene position: 2434056-2435099 (Clockwise)

Preceding gene: 94311157

Following gene: 94311159

Centisome position: 61.97

GC content: 66.57

Gene sequence:

>1044_bases
ATGACGACCAACCAAAGCACCCTTAACGCGCGGCTCGCGCAACCCGCCCCCAGGACCGTCGCCCCACGCGTGACGGTCTG
GCCCACGGGCATCGTGGCGGCACTGGCCTGGGGCGTGTTCGGCGCCGTGACCTGGCGCTGGCCCAACAAGATGGCCGGGT
TCAACGACTGGGCCTATACGGAGGAACTCGGCATCGTCGTGCTCGGCGTGTCCGCCGCGCTGCTATTGCTGGCCCTGGCG
GGCGGGTACGCAAGCCGGAGCGGACCGCTTCAGCACGCGGTCGCCACGCTGCGGGCAGCCGGTCCCTGGCTCGTCGCCCT
GCCATTGGTCTTCACCGCGTGGGAGATCCTGACCGCGAAGATGGCAATCCTGCCGACGCCATTCTTCGCGCCGCCCCAGG
CGCTGATCGAGGTCTACCTCGACGACTGGCAACGTCTGGGCGACAGCGCATTCAACACGTTGAAGCTGCTCGGCCTTGGC
GTGGGATACGGCGCGCTGGCCGGCTTCCTGATCGGTGTCTCGATCGGATGGTCGCGCCGCATCGGCTACTGGATTCACCC
CGTGCTGCGTGTGCTTGGCCCCCTGCCCTCCACGGCGCTGCTGCCGCTCACGTTCTACTTCTTTCCGTCGAGCTATTCCG
CAGCGGTGTTCCTGATCGCGCTGGCCACCGCGTTTCCGGTGGCGGTGCTGACGTGGTCGGGCGTGGCCGGCGTCAACAAG
AGCTACTACGACGTGGCGCGCACGATGGGTGCATCGGGCTGGTTCCTGGTGCTGCGGGTGGCGATCCCCGCCGCGCTGCC
GCAGGTATTCGTCGGCCTGTTCATGGGGCTTGGCGCATCGTTCTCCGTGCTCGTCACGGCCGAGATGATGGGCGTGAAAT
CCGGTCTGGGCTGGTACCTGACCTGGGCTCAGGGCTGGGCATCGTACGTGAACATGTATGCAGCGCTGATCGTCATGGCA
CTGCTGTTCTCGGGGGTCATCACATTGCTGTTCGCCGTGCGCGACCGCGCGCTCTCCTGGCAGAAAGGAACCGTCAAATG
GTAG

Upstream 100 bases:

>100_bases
TTCTACGCCGACGACTTCCGCCGCATTGGTGTGCTCAAGAAGTCCACCGATGCCAAACGCCTGGCGCAGCATGTCTACGC
CAACGTGCTGGGGTAAGACC

Downstream 100 bases:

>100_bases
CCGTACTGGAACACGCCCCCGAGGCCGCTGGCGCCCGGATCGACATTCGCGGTGTCAGCCACGCCTTCGGCGACGGCGCG
CGGCGGCTGCCGGTGCTGGA

Product: nitrate/sulfonate/bicarbonate family ABC transporter inner membrane subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYTEELGIVVLGVSAALLLLALA
GGYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAKMAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLG
VGYGALAGFLIGVSIGWSRRIGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNK
SYYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYLTWAQGWASYVNMYAALIVMA
LLFSGVITLLFAVRDRALSWQKGTVKW

Sequences:

>Translated_347_residues
MTTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYTEELGIVVLGVSAALLLLALA
GGYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAKMAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLG
VGYGALAGFLIGVSIGWSRRIGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNK
SYYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYLTWAQGWASYVNMYAALIVMA
LLFSGVITLLFAVRDRALSWQKGTVKW
>Mature_346_residues
TTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYTEELGIVVLGVSAALLLLALAG
GYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAKMAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLGV
GYGALAGFLIGVSIGWSRRIGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNKS
YYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYLTWAQGWASYVNMYAALIVMAL
LFSGVITLLFAVRDRALSWQKGTVKW

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081802, Length=134, Percent_Identity=34.3283582089552, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786564, Length=200, Percent_Identity=30.5, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37320; Mature: 37189

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYT
CCCCCCHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCHH
EELGIVVLGVSAALLLLALAGGYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAK
HHCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLGVGYGALAGFLIGVSIGWSRR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
IGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNK
CHHHHHHHHHHHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SYYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
TWAQGWASYVNMYAALIVMALLFSGVITLLFAVRDRALSWQKGTVKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYT
CCCCCHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCHH
EELGIVVLGVSAALLLLALAGGYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAK
HHCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLGVGYGALAGFLIGVSIGWSRR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
IGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNK
CHHHHHHHHHHHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SYYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
TWAQGWASYVNMYAALIVMALLFSGVITLLFAVRDRALSWQKGTVKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8437564 [H]