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The map label for this gene is rnhA

Identifier: 94311146

GI number: 94311146

Start: 2420845

End: 2421282

Strand: Direct

Name: rnhA

Synonym: Rmet_2208

Alternate gene names: 94311146

Gene position: 2420845-2421282 (Clockwise)

Preceding gene: 94311145

Following gene: 94311147

Centisome position: 61.63

GC content: 63.93

Gene sequence:

>438_bases
ATGCAAGAAGTCACGATTTACTCCGACGGAGCCTGCAAGGGCAACCCCGGCCCCGGCGGCTGGGGCGCCGTGCTCGTCGC
CGGCGGCCACGAGAAAGAACTGTTCGGCGGCGAAAGCCCCACCACCAACAACCGCATGGAGCTGATGGCGGTCATCGAGG
CGCTGCGCGCGCTCAAGCGCCCATGCATCGTCAATATCTATACGGACTCGCAATACGTGCAGAAAGGCATCAGCGAGTGG
ATTCACGGCTGGAAGGCACGCGGCTGGAAGACGGCGGACAAGAAGCCGGTCAAGAACGCCGACCTCTGGCAGGCACTGGA
CGAAGCCCAGAAACCTCACCAGATCACGTGGCACTGGGTGCGCGGTCACAACGGGCACCCCGGCAACGAACGCGCGGATG
CGCTGGCCAATCGCGGGGTGGCGTCGATCAATACCTGA

Upstream 100 bases:

>100_bases
CAAGTCGGCGCTGAAGCCAGTAGCGGCCCCAGTTGCCACGCCCACGGGCACGCACGGCAAGACCCCTGGCCAGGACAGCT
AGGACCATCACCTTATATAC

Downstream 100 bases:

>100_bases
CCCGTCGTTTACAATCCCGCCCATGCGACAAATCGTTCTCGATACCGAAACCACCGGCCTTAATCACGCGACAGGCGACC
GACTGATCGAAATCGGCTGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKRPCIVNIYTDSQYVQKGISEW
IHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWVRGHNGHPGNERADALANRGVASINT

Sequences:

>Translated_145_residues
MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKRPCIVNIYTDSQYVQKGISEW
IHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWVRGHNGHPGNERADALANRGVASINT
>Mature_145_residues
MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKRPCIVNIYTDSQYVQKGISEW
IHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWVRGHNGHPGNERADALANRGVASINT

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=37.3239436619718, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=63.9705882352941, Blast_Score=185, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI115532542, Length=139, Percent_Identity=38.1294964028777, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=36.4864864864865, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=36.4864864864865, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_RALME (Q1LL89)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584356.1
- ProteinModelPortal:   Q1LL89
- SMR:   Q1LL89
- STRING:   Q1LL89
- GeneID:   4039025
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2208
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q1LL89
- ProtClustDB:   PRK00203
- BioCyc:   RMET266264:RMET_2208-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 15966; Mature: 15966

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKR
CCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PCIVNIYTDSQYVQKGISEWIHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWV
CEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
RGHNGHPGNERADALANRGVASINT
ECCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKR
CCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PCIVNIYTDSQYVQKGISEWIHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWV
CEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
RGHNGHPGNERADALANRGVASINT
ECCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA