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The map label for this gene is gloB

Identifier: 94311144

GI number: 94311144

Start: 2419219

End: 2420019

Strand: Reverse

Name: gloB

Synonym: Rmet_2206

Alternate gene names: 94311144

Gene position: 2420019-2419219 (Counterclockwise)

Preceding gene: 94311148

Following gene: 94311143

Centisome position: 61.61

GC content: 68.16

Gene sequence:

>801_bases
ATGTTGAAAGTGGAGCCGATCCCCGCCTTTCAGGACAACTATATCTGGGCAATCCACGACGGGCATAGCGCGGCCGTGGT
GGACCCAGGCGAGGCCGCACCGGTCGAGCAGTTTCTGGCGCAACGCGGGCTGGCACTGGGCGCTATTGTAATCACCCATC
ACCATGGGGATCACCAAGGTGGTGTATCAGACCTCCTCGCGCGTCATCCGAGTGGGCCGAACGGCGAGCCGCTGCCGGTG
ATCGGTCCGCAAGGCGAGCGAATTGGCCACCGCACGCAGGCCGTGCGCGAAGGCGACACGGTCACGCTGCGTCATCCGGC
AGTGACGTTCCGGGTACTGGATGTTCCGGGTCACACCGCAGGCCATGTGGCCTATGTCGGCGACCTGCCTGGCGCCGGCC
CCGTGGTGTTCTGTGGCGACACGCTGTTCGCCAGCGGCTGCGGGCGCCTGTTCGAAGGCACGCCGGCCCAGATGCTGGCA
TCCCTGGACAAGCTCGCGGCACTGCCCGGCGACACGCGCGTCTACTGCGCCCACGAGTACACGCGCAGCAACGTCCGCTT
TGCCCAGGCCGTCGAGCCGGCCAACCCGGATCTGGCGGCGTGGGCGGCGCGGGTGGATATCCTGCGCGAGGCCGGCACGC
CTACCGTGCCAACGACCGTGGCGCACGAGCGCGCGGTCAATCCGTTCTTGCGGTCCCGCGAGTCCACGGTCCGCCAGGCT
GTGCGGGCGCAGGGCGGTGACGTGAGCGGTGACGCCGCGGCTTTTGGTGCGCTGCGAGGCTGGAAGGACGGCTTTCGTTA
A

Upstream 100 bases:

>100_bases
CGTACTGCTGGCCTTCCCACTCGAGCACATACTGCCCGGGTGGCGAGGCTAGCCATTCTTCCCAATCTATAATCGGTCGG
TTGGACATAGAGGAGCACGC

Downstream 100 bases:

>100_bases
TGCGGATGGGACAATCCGGTTGCGGGCTGGGCGGGGCAATTGATTCAAATTGCATCATGGGGAAAATCTCATTGACGCGA
GGGGGGCTTTTCCATAGCAT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQGGVSDLLARHPSGPNGEPLPV
IGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTAGHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLA
SLDKLAALPGDTRVYCAHEYTRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQA
VRAQGGDVSGDAAAFGALRGWKDGFR

Sequences:

>Translated_266_residues
MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQGGVSDLLARHPSGPNGEPLPV
IGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTAGHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLA
SLDKLAALPGDTRVYCAHEYTRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQA
VRAQGGDVSGDAAAFGALRGWKDGFR
>Mature_266_residues
MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQGGVSDLLARHPSGPNGEPLPV
IGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTAGHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLA
SLDKLAALPGDTRVYCAHEYTRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQA
VRAQGGDVSGDAAAFGALRGWKDGFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=270, Percent_Identity=39.6296296296296, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=39.6296296296296, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI14150041, Length=246, Percent_Identity=39.4308943089431, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI116642887, Length=273, Percent_Identity=36.996336996337, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI21703352, Length=247, Percent_Identity=38.0566801619433, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI46361987, Length=209, Percent_Identity=38.2775119617225, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786406, Length=266, Percent_Identity=40.6015037593985, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17536925, Length=276, Percent_Identity=34.4202898550725, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324614, Length=250, Percent_Identity=30.8, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=27.8810408921933, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356335, Length=270, Percent_Identity=38.8888888888889, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24667711, Length=270, Percent_Identity=38.8888888888889, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24667703, Length=270, Percent_Identity=38.8888888888889, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_RALME (Q1LL91)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584354.1
- ProteinModelPortal:   Q1LL91
- SMR:   Q1LL91
- STRING:   Q1LL91
- GeneID:   4039023
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2206
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   FGHEYTL
- PhylomeDB:   Q1LL91
- ProtClustDB:   CLSK896990
- BioCyc:   RMET266264:RMET_2206-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28137; Mature: 28137

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQG
CCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCC
GVSDLLARHPSGPNGEPLPVIGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTA
CHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCC
GHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLASLDKLAALPGDTRVYCAHEY
CCEEEEECCCCCCCEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
TRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQA
CCCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHH
VRAQGGDVSGDAAAFGALRGWKDGFR
HHHCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQG
CCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCC
GVSDLLARHPSGPNGEPLPVIGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTA
CHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCC
GHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLASLDKLAALPGDTRVYCAHEY
CCEEEEECCCCCCCEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
TRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQA
CCCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHH
VRAQGGDVSGDAAAFGALRGWKDGFR
HHHCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA