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The map label for this gene is mltD [H]

Identifier: 94311143

GI number: 94311143

Start: 2417370

End: 2419082

Strand: Reverse

Name: mltD [H]

Synonym: Rmet_2205

Alternate gene names: 94311143

Gene position: 2419082-2417370 (Counterclockwise)

Preceding gene: 94311144

Following gene: 94311142

Centisome position: 61.58

GC content: 65.38

Gene sequence:

>1713_bases
ATGCGATTTGGTCGATTTCTGGCGGTAGTTGCGTGTGCCACGCTGCTTGCAGCCTGCGCCAGCACACCCACACCGCCCGC
CGGCGAATTGTCCGGCACGCCGACCGCCCTGGAATCCGGTCCGAAGAAGGACCCGCTCAATACCCTCAACAGCACCGGCC
GCGCCGGCACGTTATCCCGTTCCACCGTCGTGAATGTCGACCAGTCCAGCGTGGACTGGCTGCGCGGGCCGTCCAGCGAT
ATCTGGGACCGCATCCGCAAGGGTTTCGCGATGCCCGACCTGGAAGGCACGCTGGTCGATGATCGCACGCAGTGGTACGC
GGCCCGCCCTGACTATATGGAGCGGATGGTGGGGCGGTCGAGCCGCTACCTCTACCACATCGTCGAGGAGCTCGAACGGC
GCAAGATGCCGACCGAGCTGGCCCTGCTGCCGTTCGTGGAGAGCGCATTCAATCCCCAGGCGGAATCCACGGCCAAGGCG
GCCGGCATGTGGCAGTTCATCCCGAGCACGGGCAAGACGTACAACCTCAAGCAGAACATGTTCCGCGACGAGCGGCGCGA
CGTGCTGGCATCGACCGACGCGGCCCTCGACTATCTGCAGCGGCTCTACGACATGTTCGGCGACTGGCAATTGGCGCTGG
CCGCCTACAACTGGGGCGAAGGCGCCGTGTCGCGAGCCATCGCGCGTAATCAGGCCCGGGGGCTGCCCACGGACTACGCC
AGCCTGACCATGCCGACCGAGACGCGCTATTACGTGCCGAAGCTCCAGGCGGTGAAGAACATCATCGCCAACCCGGCCGC
GTACGGCGTCAGGCTGCCTGAGATCCCCGATCATCCGTACTTCGTGACGGTCACGACCTCGCGGGATATCGACGTGGCGC
TGGCTGCCAAGCTGGCCAACATGCCGCTGGACGAGTTCAAGGCGCTCAATCCGTCGTTCAACCGACCGGTGATCCTGGGT
GCGGCCAATCCGCAGATCCTGCTGCCGTTCGAGAACGCCGAGCGCTTCCAGTACAACCTCAATACCTATCGTGGCGGGTT
GTCGAGCTGGACGGCGGTGACCGTGGATAGCCGTGAACGTGTGGAGTCGCTGGCCGCGCGGCTCAACGTGGATGTGGACA
CGCTGCGCGAGATCAACCGGATTCCCAAGGGGATGCGCCTGAAAGCCGGTTCGACCGTCATGATTCCGCGTACCGAGCGC
CACGACCAGGACATCAGCGCATCGCTGGTCGATACGGCCATGCTGGCCGTGGAGCCAGACGTGCCTGACGCCAAGCGCGT
CGTGGTGCGGGCGGGGCGCAAGGATACGGTGTCCACGGTGGCGCGTCGCTATGGCGTGTCGGTCAAGCAGGTGCAGTCGT
GGAACAAGCTCTCCGGCAGCAAGCTTGTTGCCGGCCAGAGTCTGGTGCTGATGCTGCGTCCGAACTCCGCCGCCGCCATG
CGGGCGGAGCGCGCCGGGGACGAAGACGACACGCCAGCCAAGGCCGCGCCACATGGCAAGTCGGGTGGCAAGACACAGGC
CGTGGCAAAGTCGGAGAAATACGATAAGGTCGAGAAGTCGTCGCGATCGGCCAAGGCCGAACCCCGCAAGCGGGTGGTGG
TGGAGGCCTCGCCGCACAGTTCCACGAGCAAGTCGTCGGGCAAGTCCGCCACGCGCGAAAGCAAGTCGTCGCCGACGCCA
GCGACCAAATCCAAGTCGAGCCGCGCGCAGTAG

Upstream 100 bases:

>100_bases
CAATTGATTCAAATTGCATCATGGGGAAAATCTCATTGACGCGAGGGGGGCTTTTCCATAGCATCAGGCCAACTTTTTGG
CGTCACTTTCAGAGAACTTA

Downstream 100 bases:

>100_bases
CATCTGCGCGCGGCCACCTCCTGACGCCGCGAAATTCTGGCAACCCGGTTCCACGCTAGACGCTGGGCCGGGGACGCGCC
GTGCCTCCGTCATGCAAATT

Product: soluble lytic murein transglycosylase or related regulatory protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRFGRFLAVVACATLLAACASTPTPPAGELSGTPTALESGPKKDPLNTLNSTGRAGTLSRSTVVNVDQSSVDWLRGPSSD
IWDRIRKGFAMPDLEGTLVDDRTQWYAARPDYMERMVGRSSRYLYHIVEELERRKMPTELALLPFVESAFNPQAESTAKA
AGMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLQRLYDMFGDWQLALAAYNWGEGAVSRAIARNQARGLPTDYA
SLTMPTETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVALAAKLANMPLDEFKALNPSFNRPVILG
AANPQILLPFENAERFQYNLNTYRGGLSSWTAVTVDSRERVESLAARLNVDVDTLREINRIPKGMRLKAGSTVMIPRTER
HDQDISASLVDTAMLAVEPDVPDAKRVVVRAGRKDTVSTVARRYGVSVKQVQSWNKLSGSKLVAGQSLVLMLRPNSAAAM
RAERAGDEDDTPAKAAPHGKSGGKTQAVAKSEKYDKVEKSSRSAKAEPRKRVVVEASPHSSTSKSSGKSATRESKSSPTP
ATKSKSSRAQ

Sequences:

>Translated_570_residues
MRFGRFLAVVACATLLAACASTPTPPAGELSGTPTALESGPKKDPLNTLNSTGRAGTLSRSTVVNVDQSSVDWLRGPSSD
IWDRIRKGFAMPDLEGTLVDDRTQWYAARPDYMERMVGRSSRYLYHIVEELERRKMPTELALLPFVESAFNPQAESTAKA
AGMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLQRLYDMFGDWQLALAAYNWGEGAVSRAIARNQARGLPTDYA
SLTMPTETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVALAAKLANMPLDEFKALNPSFNRPVILG
AANPQILLPFENAERFQYNLNTYRGGLSSWTAVTVDSRERVESLAARLNVDVDTLREINRIPKGMRLKAGSTVMIPRTER
HDQDISASLVDTAMLAVEPDVPDAKRVVVRAGRKDTVSTVARRYGVSVKQVQSWNKLSGSKLVAGQSLVLMLRPNSAAAM
RAERAGDEDDTPAKAAPHGKSGGKTQAVAKSEKYDKVEKSSRSAKAEPRKRVVVEASPHSSTSKSSGKSATRESKSSPTP
ATKSKSSRAQ
>Mature_570_residues
MRFGRFLAVVACATLLAACASTPTPPAGELSGTPTALESGPKKDPLNTLNSTGRAGTLSRSTVVNVDQSSVDWLRGPSSD
IWDRIRKGFAMPDLEGTLVDDRTQWYAARPDYMERMVGRSSRYLYHIVEELERRKMPTELALLPFVESAFNPQAESTAKA
AGMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLQRLYDMFGDWQLALAAYNWGEGAVSRAIARNQARGLPTDYA
SLTMPTETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVALAAKLANMPLDEFKALNPSFNRPVILG
AANPQILLPFENAERFQYNLNTYRGGLSSWTAVTVDSRERVESLAARLNVDVDTLREINRIPKGMRLKAGSTVMIPRTER
HDQDISASLVDTAMLAVEPDVPDAKRVVVRAGRKDTVSTVARRYGVSVKQVQSWNKLSGSKLVAGQSLVLMLRPNSAAAM
RAERAGDEDDTPAKAAPHGKSGGKTQAVAKSEKYDKVEKSSRSAKAEPRKRVVVEASPHSSTSKSSGKSATRESKSSPTP
ATKSKSSRAQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=413, Percent_Identity=35.5932203389831, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 62517; Mature: 62517

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFGRFLAVVACATLLAACASTPTPPAGELSGTPTALESGPKKDPLNTLNSTGRAGTLSR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCCCCCCCCC
STVVNVDQSSVDWLRGPSSDIWDRIRKGFAMPDLEGTLVDDRTQWYAARPDYMERMVGRS
CEEEECCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCEEECCHHHHHHHHCCC
SRYLYHIVEELERRKMPTELALLPFVESAFNPQAESTAKAAGMWQFIPSTGKTYNLKQNM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCEECHHHHH
FRDERRDVLASTDAALDYLQRLYDMFGDWQLALAAYNWGEGAVSRAIARNQARGLPTDYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
SLTMPTETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVALAAKLAN
EEECCCCCCEECHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHC
MPLDEFKALNPSFNRPVILGAANPQILLPFENAERFQYNLNTYRGGLSSWTAVTVDSRER
CCHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHH
VESLAARLNVDVDTLREINRIPKGMRLKAGSTVMIPRTERHDQDISASLVDTAMLAVEPD
HHHHHHHHCCCHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHEEECCC
VPDAKRVVVRAGRKDTVSTVARRYGVSVKQVQSWNKLSGSKLVAGQSLVLMLRPNSAAAM
CCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCEEEEEECCCCHHHH
RAERAGDEDDTPAKAAPHGKSGGKTQAVAKSEKYDKVEKSSRSAKAEPRKRVVVEASPHS
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
STSKSSGKSATRESKSSPTPATKSKSSRAQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFGRFLAVVACATLLAACASTPTPPAGELSGTPTALESGPKKDPLNTLNSTGRAGTLSR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCCCCCCCCC
STVVNVDQSSVDWLRGPSSDIWDRIRKGFAMPDLEGTLVDDRTQWYAARPDYMERMVGRS
CEEEECCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCEEECCHHHHHHHHCCC
SRYLYHIVEELERRKMPTELALLPFVESAFNPQAESTAKAAGMWQFIPSTGKTYNLKQNM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCEECHHHHH
FRDERRDVLASTDAALDYLQRLYDMFGDWQLALAAYNWGEGAVSRAIARNQARGLPTDYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
SLTMPTETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVALAAKLAN
EEECCCCCCEECHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHC
MPLDEFKALNPSFNRPVILGAANPQILLPFENAERFQYNLNTYRGGLSSWTAVTVDSRER
CCHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHH
VESLAARLNVDVDTLREINRIPKGMRLKAGSTVMIPRTERHDQDISASLVDTAMLAVEPD
HHHHHHHHCCCHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHEEECCC
VPDAKRVVVRAGRKDTVSTVARRYGVSVKQVQSWNKLSGSKLVAGQSLVLMLRPNSAAAM
CCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCEEEEEECCCCHHHH
RAERAGDEDDTPAKAAPHGKSGGKTQAVAKSEKYDKVEKSSRSAKAEPRKRVVVEASPHS
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
STSKSSGKSATRESKSSPTPATKSKSSRAQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]