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The map label for this gene is echA12 [H]

Identifier: 94311138

GI number: 94311138

Start: 2410065

End: 2410916

Strand: Reverse

Name: echA12 [H]

Synonym: Rmet_2200

Alternate gene names: 94311138

Gene position: 2410916-2410065 (Counterclockwise)

Preceding gene: 94311139

Following gene: 94311137

Centisome position: 61.38

GC content: 64.08

Gene sequence:

>852_bases
ATGAGCGATATTGCACTGGACATGCGCCACCACAAGCGCGCCTTTGCGGACTATCAACCGAAGCATTTCCTGTGGTCCGT
CTCGGCGGACGGCAAGGTCGCCACGATCACGCTGAACCGGCCGGAGCGCAAGAATCCGCTGACGTTCGATTCCTACGCCG
AGCTGCGTGATCTGTTCCGTAGTCTGTGCTACGCCACCGACATCAAGACCGTGGTGGTGACCGGCGCGGGCGGGAATTTC
TGCTCTGGCGGCGATGTACACGAGATCATTGGCCCGCTGACGCGCATGACCATGCCGGAGCTGCTCGACTTCACGCGCAT
GACCGGCGACTTGATCAAGGCCATGCGCGCCTGCCCGCAGCCGATCATCAGCGCGATCGACGGCATCTGCGCCGGCGCCG
GCGCGATGATCGCGCTGGCCTCCGATATGCGTCTGGGTACCGCGCTGGCGAAGACCGCTTTCCTGTTCGTGCGCGTTGGT
CTGGCCGGCGCCGATATGGGCGCATGTTCGCTGCTGCCCCGCGTGATTGGGCAGGGGAGAGCGAGCGAGCTGCTTTACAC
CGGCCGCTCGATGAGCGCCGAGGAAGGGCTGCAATGGGGCTTCTTCAACGCGCTGCACGCTTCCGAAACGCTGCTGGCCG
AGGCGCAGTCGCTGGCCACGCAGATCGCCGCGGGTCCGACTTTCGCGCATGGCGTTACCAAGAAGCTGCTCCATCAGGAG
TGGAATATGGGGCTCGACGAAGCGATCGAGGCCGAAGCGGAGGCACAGGCCATCTGCATGCAGACTCGCGACTTCCGGCG
AGCCTATGACGCGTTCGTCGCGAAGCAGAAGCCGGTGTTCGAAGGCGATTGA

Upstream 100 bases:

>100_bases
CCAGCCCTCGGCGTCCGCGATCACGGGGCAAGCCGTGCCGGTGGCCGGCGGCGAGGTGATGGCGGGCTGAACGATCAAAC
AGAGTGGAGTGAGACAACAC

Downstream 100 bases:

>100_bases
GGGGCTGGCATGAGTGACAAGACCTACCTTGAACTACCGTTCTTCGACGATACGCACCGTTCGCTCGAACGCGATCTGGA
TGCCTGGTGCGGCGAGAACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFRSLCYATDIKTVVVTGAGGNF
CSGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQPIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVG
LAGADMGACSLLPRVIGQGRASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQE
WNMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD

Sequences:

>Translated_283_residues
MSDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFRSLCYATDIKTVVVTGAGGNF
CSGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQPIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVG
LAGADMGACSLLPRVIGQGRASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQE
WNMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD
>Mature_282_residues
SDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFRSLCYATDIKTVVVTGAGGNFC
SGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQPIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVGL
AGADMGACSLLPRVIGQGRASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQEW
NMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=30.2238805970149, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=24.3027888446215, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=25.4901960784314, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=242, Percent_Identity=25.2066115702479, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI116235487, Length=184, Percent_Identity=28.2608695652174, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=34.5098039215686, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=27.2373540856031, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=24.4444444444444, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=25.0950570342205, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=30.5418719211823, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=23.5897435897436, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=33.502538071066, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=25.5725190839695, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=27.6264591439689, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=26.7241379310345, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=25.2747252747253, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=23.8866396761134, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=23.7154150197628, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920382, Length=281, Percent_Identity=28.1138790035587, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357171, Length=210, Percent_Identity=30.952380952381, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=30.2681992337165, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24650670, Length=201, Percent_Identity=30.3482587064677, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=26.2548262548263, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=26.2548262548263, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=24.21875, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=23.3962264150943, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30695; Mature: 30563

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFR
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
SLCYATDIKTVVVTGAGGNFCSGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQ
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVGLAGADMGACSLLPRVIGQGR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
ASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WNMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFR
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
SLCYATDIKTVVVTGAGGNFCSGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQ
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVGLAGADMGACSLLPRVIGQGR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
ASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WNMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]