Click here to switch to the map view.

The map label for this gene is fadE25 [H]

Identifier: 94311137

GI number: 94311137

Start: 2408889

End: 2410055

Strand: Reverse

Name: fadE25 [H]

Synonym: Rmet_2199

Alternate gene names: 94311137

Gene position: 2410055-2408889 (Counterclockwise)

Preceding gene: 94311138

Following gene: 94311136

Centisome position: 61.35

GC content: 67.1

Gene sequence:

>1167_bases
ATGAGTGACAAGACCTACCTTGAACTACCGTTCTTCGACGATACGCACCGTTCGCTCGAACGCGATCTGGATGCCTGGTG
CGGCGAGAACCTTTGCGGCGTCGATCACCACGATGCCGACGCGGCGTGCCGCACGCTGGTGCGCCAGCTTGGGCAGGCGG
GATGGCTCCGCTACTGCGTGCCGGCGGCGTTCGGCGGCGCGCTACCCGCGCTCGATTCGCGCGCGCTTTGCCTGCTGCGC
GAGACACTGGCGCGCCATGACGGTCTGGCTGACTTTGCTTTCGCGATGCAGGGCCTTGGCTCGGGCGCGATCACGCTGGC
CGGCAGCGACGCGGTGCGCGAGCGCTATCTTCCGCGCGTGGCGAATGGCGAGGCCATTGCGGCATTCGCGCTGTCCGAGC
CCGAGGCGGGTTCTGACGTGGCGGCGATGCAATGCAGCGCGAAGCTGTCCGATGACGGCACGCACTACGTGCTGAACGGC
GCCAAGACGTGGATATCTAATGGCGGCATCGCCGACTTCTATTGCGTGTTCGCGCGCACTGGGGAGGCAGCCCGCGCGCG
CGGCATCACCGCGTTCGTGGTGGATGCCAATACTCCGGGCCTGTCCATTGCAGAGCGCATCGACGTGATCGCGCCACATC
CTCTGGCAACACTGCGTTTCGATGACTGCCGCGTACCCGTTGGCAACCGCCTGGGCGAGCCGGGGCAGGGCTTCAAGGTG
GCGATGATGACACTCGACATCTTCCGGGCCTCGGTGGCCGCCGCCGCGCTTGGCTTCGGTCGCGCGGCGCTGGACGATGC
GCTGGCACGCGCCACGTCGCGCCCGATGTTCGGCGGCGTGCTGGCGGATCTGCAACTCACGCAGGCGGCGATCGGTGACA
TGGCTACGGCCATTGATGCCGCCGCGCTGCTGACTTATCGCGCCGCCTGGCTGCGCGATGTGAGGCAGCAGCGCACGACG
CGCGAGGCGGCGATGGCCAAGATGGTCGCCACCGAGAACGCGCAGCAGGTCATCGACCGGGCCGTGCAGATGTTCGGCGG
CCTCGGCGTCAAGGTTGGCACGCGTGTGGAAAGCCTCTACCGCGAGATTCGCTCGCTGCGGATCTACGAAGGCGCCACTG
AAGTGCAGAAGCTGATCATTGCGCGCGAGACGCTGGCAGCCCGATAG

Upstream 100 bases:

>100_bases
GGCACAGGCCATCTGCATGCAGACTCGCGACTTCCGGCGAGCCTATGACGCGTTCGTCGCGAAGCAGAAGCCGGTGTTCG
AAGGCGATTGAGGGGCTGGC

Downstream 100 bases:

>100_bases
AACACGAGACAGGAGACAAGTATGGCCGCCCCCCAACCAGCCACCGCTCATGTTGATACCTTCGCACGGGACAGGTTGCC
GCCGCCGCAGACGTGGCCGG

Product: acyl-CoA dehydrogenase-like protein

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSDKTYLELPFFDDTHRSLERDLDAWCGENLCGVDHHDADAACRTLVRQLGQAGWLRYCVPAAFGGALPALDSRALCLLR
ETLARHDGLADFAFAMQGLGSGAITLAGSDAVRERYLPRVANGEAIAAFALSEPEAGSDVAAMQCSAKLSDDGTHYVLNG
AKTWISNGGIADFYCVFARTGEAARARGITAFVVDANTPGLSIAERIDVIAPHPLATLRFDDCRVPVGNRLGEPGQGFKV
AMMTLDIFRASVAAAALGFGRAALDDALARATSRPMFGGVLADLQLTQAAIGDMATAIDAAALLTYRAAWLRDVRQQRTT
REAAMAKMVATENAQQVIDRAVQMFGGLGVKVGTRVESLYREIRSLRIYEGATEVQKLIIARETLAAR

Sequences:

>Translated_388_residues
MSDKTYLELPFFDDTHRSLERDLDAWCGENLCGVDHHDADAACRTLVRQLGQAGWLRYCVPAAFGGALPALDSRALCLLR
ETLARHDGLADFAFAMQGLGSGAITLAGSDAVRERYLPRVANGEAIAAFALSEPEAGSDVAAMQCSAKLSDDGTHYVLNG
AKTWISNGGIADFYCVFARTGEAARARGITAFVVDANTPGLSIAERIDVIAPHPLATLRFDDCRVPVGNRLGEPGQGFKV
AMMTLDIFRASVAAAALGFGRAALDDALARATSRPMFGGVLADLQLTQAAIGDMATAIDAAALLTYRAAWLRDVRQQRTT
REAAMAKMVATENAQQVIDRAVQMFGGLGVKVGTRVESLYREIRSLRIYEGATEVQKLIIARETLAAR
>Mature_387_residues
SDKTYLELPFFDDTHRSLERDLDAWCGENLCGVDHHDADAACRTLVRQLGQAGWLRYCVPAAFGGALPALDSRALCLLRE
TLARHDGLADFAFAMQGLGSGAITLAGSDAVRERYLPRVANGEAIAAFALSEPEAGSDVAAMQCSAKLSDDGTHYVLNGA
KTWISNGGIADFYCVFARTGEAARARGITAFVVDANTPGLSIAERIDVIAPHPLATLRFDDCRVPVGNRLGEPGQGFKVA
MMTLDIFRASVAAAALGFGRAALDDALARATSRPMFGGVLADLQLTQAAIGDMATAIDAAALLTYRAAWLRDVRQQRTTR
EAAMAKMVATENAQQVIDRAVQMFGGLGVKVGTRVESLYREIRSLRIYEGATEVQKLIIARETLAAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=344, Percent_Identity=40.406976744186, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI187960098, Length=346, Percent_Identity=35.5491329479769, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4557231, Length=346, Percent_Identity=35.5491329479769, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4557235, Length=340, Percent_Identity=36.7647058823529, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI76496475, Length=340, Percent_Identity=36.7647058823529, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI226958412, Length=380, Percent_Identity=32.6315789473684, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI21361497, Length=342, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI226958414, Length=340, Percent_Identity=34.7058823529412, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI7656849, Length=377, Percent_Identity=31.2997347480106, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI4501859, Length=343, Percent_Identity=33.5276967930029, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI4501857, Length=384, Percent_Identity=27.34375, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI48976061, Length=291, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI211971008, Length=291, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4503943, Length=347, Percent_Identity=29.971181556196, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI7669494, Length=315, Percent_Identity=30.7936507936508, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI38505218, Length=292, Percent_Identity=28.4246575342466, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081958, Length=345, Percent_Identity=28.695652173913, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786223, Length=264, Percent_Identity=28.4090909090909, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081702, Length=267, Percent_Identity=27.7153558052434, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082384, Length=281, Percent_Identity=24.1992882562278, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71985184, Length=345, Percent_Identity=34.4927536231884, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17569725, Length=348, Percent_Identity=34.4827586206897, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17534899, Length=344, Percent_Identity=34.593023255814, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17570075, Length=347, Percent_Identity=34.2939481268012, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17508101, Length=348, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17538396, Length=352, Percent_Identity=32.6704545454545, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17533517, Length=281, Percent_Identity=34.5195729537367, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17506239, Length=379, Percent_Identity=30.8707124010554, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71985192, Length=302, Percent_Identity=31.7880794701987, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71990804, Length=226, Percent_Identity=36.283185840708, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17505929, Length=382, Percent_Identity=27.7486910994764, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17551932, Length=342, Percent_Identity=28.0701754385965, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982178, Length=212, Percent_Identity=33.4905660377358, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563615, Length=233, Percent_Identity=30.9012875536481, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17534353, Length=283, Percent_Identity=28.6219081272085, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562446, Length=296, Percent_Identity=31.7567567567568, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531909, Length=242, Percent_Identity=30.9917355371901, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI86563383, Length=273, Percent_Identity=26.007326007326, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI86563381, Length=273, Percent_Identity=26.007326007326, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356377, Length=382, Percent_Identity=37.9581151832461, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24646207, Length=381, Percent_Identity=37.007874015748, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI281363737, Length=330, Percent_Identity=35.4545454545455, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24660351, Length=347, Percent_Identity=34.5821325648415, Blast_Score=188, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24666513, Length=277, Percent_Identity=38.2671480144404, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21355753, Length=356, Percent_Identity=32.0224719101124, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920834, Length=347, Percent_Identity=30.5475504322767, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 41543; Mature: 41412

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKTYLELPFFDDTHRSLERDLDAWCGENLCGVDHHDADAACRTLVRQLGQAGWLRYCV
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
PAAFGGALPALDSRALCLLRETLARHDGLADFAFAMQGLGSGAITLAGSDAVRERYLPRV
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCC
ANGEAIAAFALSEPEAGSDVAAMQCSAKLSDDGTHYVLNGAKTWISNGGIADFYCVFART
CCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHCCCCCHHHEEEECCC
GEAARARGITAFVVDANTPGLSIAERIDVIAPHPLATLRFDDCRVPVGNRLGEPGQGFKV
CCHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
AMMTLDIFRASVAAAALGFGRAALDDALARATSRPMFGGVLADLQLTQAAIGDMATAIDA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALLTYRAAWLRDVRQQRTTREAAMAKMVATENAQQVIDRAVQMFGGLGVKVGTRVESLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
REIRSLRIYEGATEVQKLIIARETLAAR
HHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDKTYLELPFFDDTHRSLERDLDAWCGENLCGVDHHDADAACRTLVRQLGQAGWLRYCV
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
PAAFGGALPALDSRALCLLRETLARHDGLADFAFAMQGLGSGAITLAGSDAVRERYLPRV
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCC
ANGEAIAAFALSEPEAGSDVAAMQCSAKLSDDGTHYVLNGAKTWISNGGIADFYCVFART
CCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHCCCCCHHHEEEECCC
GEAARARGITAFVVDANTPGLSIAERIDVIAPHPLATLRFDDCRVPVGNRLGEPGQGFKV
CCHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
AMMTLDIFRASVAAAALGFGRAALDDALARATSRPMFGGVLADLQLTQAAIGDMATAIDA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALLTYRAAWLRDVRQQRTTREAAMAKMVATENAQQVIDRAVQMFGGLGVKVGTRVESLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
REIRSLRIYEGATEVQKLIIARETLAAR
HHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]