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The map label for this gene is phoB [H]

Identifier: 94311118

GI number: 94311118

Start: 2388147

End: 2388854

Strand: Reverse

Name: phoB [H]

Synonym: Rmet_2180

Alternate gene names: 94311118

Gene position: 2388854-2388147 (Counterclockwise)

Preceding gene: 94311119

Following gene: 94311117

Centisome position: 60.81

GC content: 61.86

Gene sequence:

>708_bases
ATGCCAAGCAGTATTCTCGTTGTCGAAGACGAACCGGCAATCGCCGAGTTGATTGCCGTCAATCTGCAGCACGCCGGGCA
CTATCCCATCCGCGCATACAACGCCGAGCAGGCGCTGTCGCTGATGAGTGACGTGCTGCCGGATCTCGTGCTGCTGGACT
GGATGCTCCCGGGCAAATCGGGTGCCACCTTCGCCAAGGAGCTGCGCAACAACGATCGCACCAAGCAGATCCCGATCATC
ATGCTCACTGCGCGCAGCGAGGAGCAGGACAAGGTGATGGGCCTGGAAGCTGGCGCGGACGACTATGTGACCAAGCCTTT
TTCGCCCAAGGAACTGCTGGCCCGGATCAAGGCGGTATTGCGCCGCCGTGCGCCGCAGCTGACCGACGACGTGGTGGCGA
TCAACGGCCTGCGTCTTGACCCGGCCACGCACCGCGTTACCGGCCAGGATGATGGTGGTCCGATCAAGCTGGATCTGGGA
CCGACGGAGTTCCGTTTGCTGCACTTCTTGATGACGCATCCGGAGCGTGTGCATAGTCGTTCACAGTTGCTGGATCAGGT
TTGGGGCGACCACGTTTTCGTGGAAGAACGGACCGTGGACGTACACATCAAGCGCCTGCGCGCCGCGCTGACCCCGGGTG
GATACAGCAACATGATCGAAACCGTGCGCGGCAGCGGCTATCGCCTGGCGCGATCGCCAAGCCACTGA

Upstream 100 bases:

>100_bases
CGTAAAGGGCACGGACGTGCGTCATGCCTCGCGCGAGGACATGGAGCGCGAAGCGCTAAGCTGATAACGCGCCCGGACTG
AGTTTGCGGAGAACAATTAC

Downstream 100 bases:

>100_bases
GCGTAACCGGCCGGCGGCGGTGCGACGAGGGCCCCGTCCGGTCGAACAGGACCATGCCGGCCACGGCTGTTTCGTGGCAA
ACTCCGTCGCAATGCGACCG

Product: DNA-binding response regulator in two-component regulatory system with PhoR (or CreC)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRNNDRTKQIPII
MLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLG
PTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH

Sequences:

>Translated_235_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRNNDRTKQIPII
MLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLG
PTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH
>Mature_234_residues
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRNNDRTKQIPIIM
LTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLGP
TEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=56.5217391304348, Blast_Score=277, Evalue=4e-76,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=42.0353982300885, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=38.0952380952381, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=38.6266094420601, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=35.3982300884956, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.0807174887892, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=34.7457627118644, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=36.0869565217391, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.3162393162393, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=34.1772151898734, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=34.070796460177, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=33.1858407079646, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=30.4166666666667, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26193; Mature: 26062

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GATFAKELRNNDRTKQIPIIMLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVL
CHHHHHHHHCCCCCCCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLGPTEFRLLHFLMTHPERVHSR
HHCCCCCCCCEEEECCEEECCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
SQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH
HHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GATFAKELRNNDRTKQIPIIMLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVL
CHHHHHHHHCCCCCCCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLGPTEFRLLHFLMTHPERVHSR
HHCCCCCCCCEEEECCEEECCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
SQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH
HHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]