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The map label for this gene is phoR [H]

Identifier: 94311117

GI number: 94311117

Start: 2386671

End: 2388023

Strand: Reverse

Name: phoR [H]

Synonym: Rmet_2179

Alternate gene names: 94311117

Gene position: 2388023-2386671 (Counterclockwise)

Preceding gene: 94311118

Following gene: 94311115

Centisome position: 60.79

GC content: 62.97

Gene sequence:

>1353_bases
ATGAATGTCATCTGGGCCCGTTCCGTGGCCATTCTCGTCAGCCTGACGGTCGTCGCCACCGGACTGTACGTCTTTGCCGG
CCCTGTCTCCGGTCTGCTGTTCCTCTGCATTGCGCTGTTTCTGCTGCTCGGCTACTACCTGTTCCAGATCAACCGGCTCT
GGAAGGTGCTTGATGCGCCCGCATACGGCGAGATTCCCAGCGCACTGGGTCTGTGGGGCGAGGTCTACTATCGCCTGCAC
CGCCTGGTGAAGCGCTGGCGCACCCAGGTGTTGCAGGTGGAGCAGCAGCATACCCGCTTTATCCAGGCCATCCAGGCATC
ACCCTATGGCGTGCTGATGCTCGACGACGCCGACCAGATTGAATGGTGCAACGACGTGGCCGAGCAGCATTTCGGCCTTA
ACGCGAAGCGCGACGTGCGTCAGCGCATCACGCACTTGATTCGGCGCCCGGAGTTCGTGCACTACCTGACGCGCGAACGC
TATGACGAGCCCCTGATCATGCGCGATATGGGCGAACACAAACGCAGCAGTACCTCGGTACAGATTCTTCCCTACGGCGA
GAATCGCAAGCTCGTGCTGACGCAGGACATCACCAAGGTCGAGAACACCGAGGCGATGCGGCGGGACTTCGTCGCCAATG
TCTCCCACGAGTTGAAGACGCCGCTGACCGTGCTGACCGGATTCCTCGAGACCGTTCGCGACCTGCCGGTGTCGGAGGAA
GATCGCAAGCGCTACATCGACATGATGCTGGTGCAGTCGATGCGGATGCAGCATATCGTGGAGGATCTTCTGGCGCTGGC
CAAGCTGGAATCGGATGTCCAGCCGCCGACGCTCGACCCAGTGGACGTCGGCGGGCTCGCCGCACACCTCGTACATGATG
CCGAGGCGTTGTCCCAAGGGCGCCACGACATCCATGCGGAGATCGATACTGCCGTGGTGCTGCGCGGCTCGGAAGGCGAG
CTGCTGTCGGCGCTTGGCAATCTGGTGTCGAACGCGGTGCGCTATACCCCGGACGGCGGGCGCATTGCCATCCGTATGAC
GTACACGGACGGACATTCGGTGTTCTCAGTGAGCGATACGGGGCTGGGGATTGCGCCCGAGCATATCCCGCGCCTGACCG
AGCGCTTCTATCGCGTTGATCGCAGCCGCTCACGGGATACCGGCGGCACGGGGCTGGGCTTGGCTATCGTCAAGCACGTC
CTGTCGCGTCACCACGCGGATCTGCGAGTGACCAGTGAAGTGGGGCGGGGCAGCACGTTCCGCATCGTGTTCCCGGTGGA
TCGCAGCGGGTATGAACCTGAATCGGCACAGCCGATGCCGCAACTGGGCGATGCCCCGCGCCACGCCGCCTGA

Upstream 100 bases:

>100_bases
GACGAGGGCCCCGTCCGGTCGAACAGGACCATGCCGGCCACGGCTGTTTCGTGGCAAACTCCGTCGCAATGCGACCGCCG
CTCGAACTTCCTACCCTCGC

Downstream 100 bases:

>100_bases
CATCAACCGCACGCTGCTTCAGCGAATCCAATACAAAAAGGCCGCTTCCTATCGGAGAAGCGGCCTTTTTGCTTGCAAGG
TGCGCCGTTCAGGTACCGAA

Product: sensory histidine kinase in two-component regulatory system with PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAPAYGEIPSALGLWGEVYYRLH
RLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQIEWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRER
YDEPLIMRDMGEHKRSSTSVQILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEE
DRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQGRHDIHAEIDTAVVLRGSEGE
LLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDTGLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHV
LSRHHADLRVTSEVGRGSTFRIVFPVDRSGYEPESAQPMPQLGDAPRHAA

Sequences:

>Translated_450_residues
MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAPAYGEIPSALGLWGEVYYRLH
RLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQIEWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRER
YDEPLIMRDMGEHKRSSTSVQILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEE
DRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQGRHDIHAEIDTAVVLRGSEGE
LLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDTGLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHV
LSRHHADLRVTSEVGRGSTFRIVFPVDRSGYEPESAQPMPQLGDAPRHAA
>Mature_450_residues
MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAPAYGEIPSALGLWGEVYYRLH
RLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQIEWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRER
YDEPLIMRDMGEHKRSSTSVQILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEE
DRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQGRHDIHAEIDTAVVLRGSEGE
LLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDTGLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHV
LSRHHADLRVTSEVGRGSTFRIVFPVDRSGYEPESAQPMPQLGDAPRHAA

Specific function: Member of the two-component regulatory system phoR/phoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates phoB in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=404, Percent_Identity=37.3762376237624, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=34.0248962655602, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693157, Length=381, Percent_Identity=28.0839895013123, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788393, Length=270, Percent_Identity=27.4074074074074, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI48994928, Length=413, Percent_Identity=24.9394673123487, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=35.1851851851852, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788713, Length=272, Percent_Identity=26.8382352941176, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788549, Length=361, Percent_Identity=24.3767313019391, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082128, Length=230, Percent_Identity=29.1304347826087, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=241, Percent_Identity=31.1203319502075, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=27.5229357798165, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=250, Percent_Identity=25.2, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787894, Length=233, Percent_Identity=29.1845493562232, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790551, Length=225, Percent_Identity=30.6666666666667, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789403, Length=225, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=26.431718061674, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=29.5964125560538, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR021766
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR014310 [H]

Pfam domain/function: PF11808 DUF3329; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50711; Mature: 50711

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AYGEIPSALGLWGEVYYRLHRLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
EWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRERYDEPLIMRDMGEHKRSSTSV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCHHCCCCCCE
QILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEE
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHH
DRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RHDIHAEIDTAVVLRGSEGELLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDT
CHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCCEEEEECCC
GLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHVLSRHHADLRVTSEVGRGSTF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHCCCCCEE
RIVFPVDRSGYEPESAQPMPQLGDAPRHAA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AYGEIPSALGLWGEVYYRLHRLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
EWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRERYDEPLIMRDMGEHKRSSTSV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCHHCCCCCCE
QILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEE
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHH
DRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RHDIHAEIDTAVVLRGSEGELLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDT
CHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCCEEEEECCC
GLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHVLSRHHADLRVTSEVGRGSTF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHCCCCCEE
RIVFPVDRSGYEPESAQPMPQLGDAPRHAA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 2115874 [H]