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The map label for this gene is fabI [H]

Identifier: 94311085

GI number: 94311085

Start: 2340195

End: 2340977

Strand: Direct

Name: fabI [H]

Synonym: Rmet_2147

Alternate gene names: 94311085

Gene position: 2340195-2340977 (Clockwise)

Preceding gene: 94311079

Following gene: 94311087

Centisome position: 59.58

GC content: 62.32

Gene sequence:

>783_bases
ATGGGATTTCTGGCAGGTAAGCGGATCCTGATTACCGGTTTGCTTTCGAACCGTTCGATCGCCTACGGCATTGCAGCAGC
GTGCAAGCGTGAAGGCGCGGAACTGGCGTTCACTTATGTCGGCGAACGATTCAAGGACCGCATCACGGACTTCGCCAAGG
AATTCGGCTCCGACATGATCTATGAGTGCGACGTAGGTAGCGATGAACAGATCGACGCCACGTTTGCCGCACTGGGGCAG
CGCTGGGAAAAATTCGACGGTCTGGTGCACTCGATCGGCTTCGCGCCGCGCGAAGCCATCGCCGGCAACTTCCTGGACGG
GCTGTCGCGTGAAGGCTTCCGGATCGCACACGATATCTCGGCCTACAGCTTCCCGGCACTGGCCAAGGCCGCGATGCCGC
TGCTCAATGACAAGGCATCGCTGCTGACGCTGACCTACCTGGGCGCCGAGCGTGTGGTACCGAACTACAACACGATGGGC
CTGGCCAAGGCTTCGCTCGAAGCCAGCGTGCGCTACCTGGCGTCGGCGGTCGGCCCGCGCGGGATGCGTGCCAACGGCAT
TTCGGCCGGTCCGATCAAGACCCTGGCCGCCTCGGGCATCAAGGACTTCAGCCGCCTGCTCAAGCATTTCGAAGACGTGG
CACCGATCCGTCGCAACGTGACGATCGAAGAAGTCGGCAACGTCGCGGCCTTCCTGCTGTCGGATCTGGCCAGCGGCGTG
ACCGGCGAGATCACGTATGTCGACGGCGGCTACAACATCGTGGGCACGGCCGTCGAGGACTGA

Upstream 100 bases:

>100_bases
CCTCTACGCGGTGCAACAAAAGCGGGAAAGTTTGCTTATTGTGCATCGGCCATGGGCCATCGCATAAAATACGCCCGCCC
CACACATATTTGGAGAATCT

Downstream 100 bases:

>100_bases
TCCCGGCTTCGCACCGCCCCTGCGATCCAGGCGCCCCAACACGGGGCGCCTTTTTGTTTCTTCAGGCCACCGCCTCGATC
GCCGCCACGGCCCGCCCGGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMIYECDVGSDEQIDATFAALGQ
RWEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDISAYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMG
LAKASLEASVRYLASAVGPRGMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGV
TGEITYVDGGYNIVGTAVED

Sequences:

>Translated_260_residues
MGFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMIYECDVGSDEQIDATFAALGQ
RWEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDISAYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMG
LAKASLEASVRYLASAVGPRGMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGV
TGEITYVDGGYNIVGTAVED
>Mature_259_residues
GFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMIYECDVGSDEQIDATFAALGQR
WEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDISAYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMGL
AKASLEASVRYLASAVGPRGMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGVT
GEITYVDGGYNIVGTAVED

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=170, Percent_Identity=35.8823529411765, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=63.2411067193676, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=27.6679841897233, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=27.1698113207547, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=27.734375, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27817; Mature: 27686

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMI
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YECDVGSDEQIDATFAALGQRWEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDIS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHCC
AYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMGLAKASLEASVRYLASAVGPR
HHHHHHHHHHHHHHHCCCHHEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TGEITYVDGGYNIVGTAVED
CCEEEEECCCCEEEEEECCC
>Mature Secondary Structure 
GFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMI
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YECDVGSDEQIDATFAALGQRWEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDIS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHCC
AYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMGLAKASLEASVRYLASAVGPR
HHHHHHHHHHHHHHHCCCHHEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TGEITYVDGGYNIVGTAVED
CCEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]