Click here to switch to the map view.

The map label for this gene is mdtK [H]

Identifier: 94311077

GI number: 94311077

Start: 2327927

End: 2329276

Strand: Direct

Name: mdtK [H]

Synonym: Rmet_2139

Alternate gene names: 94311077

Gene position: 2327927-2329276 (Clockwise)

Preceding gene: 94311076

Following gene: 94311079

Centisome position: 59.26

GC content: 62.3

Gene sequence:

>1350_bases
ATGAGCAGCGCGCTGCAGGATATTCGTCGCATTACCCATCTGGCCGCCCCCGTGCTGGTTGGACAACTGGCGGTCATCGC
CTTCGGTGTCATCGACACCATCATGTCCGGCCGCGCCTCGGCCACCGACCTTGCCGCTGTTGGCTTGGGCGGATCGATCT
ATGTCACGGTCTACGTCAGCCTGATGGGCGTGCTGCAAGCGCTCGCGCCTGTCGCGGGACAGTTGTACGGCGCCAACCGA
TCCGCGGAGATCGGCGCCGAGGTACGGCAGGCAGCATGGCTCGGTGTGGCGCTGGCTATCCCCGGCATGCTGTTGCTGCT
GTTTCCCGCACCGTTGCTGGAATTCGCCAAAGCACCACCCGAGTTGGTCGAAAAAGCATCGCACTATCTGCATATCGGTG
CATACGGCCTACCCGCAGCGCTGGGTTTTCGCATCTACATGGCGCTGAACAATGCGCTATCTCGCCCCGTCATGGTGACG
GTGCTGCAGATTGCTGGCCTGGCACTCAAGATTCCACTCAATGCCTGGTTCATCGAAGGCGGTTATGGCCTCGCCCCGAT
GGGCGGGCCAGGCTGCGCGCTGGCATCCACGCTGATCAGCTGGATATGGTTTCTGTCCGGACTGGCGATCCTGCTGCGCG
GTGCGGCGTACCGGTCCCTGGAGATCTTCACGCGTTGGGAGTGGCCACGCTGGGACCGGATTCAGCCTCTTCTGCGCCTG
GGGCTGCCGATGGGACTCACCTACCTGATCGAGATCACGTCATTCACCCTAATGTCGATCTTCATCACACGCATGGGCAC
CGATACCCTTGCGGGGCATCAGATCATCGCCAACCTCAGCGCCGTGGCGTACATGATGCCGCTCTCGCTATCCATCGCGA
CCTCGACGCTCGTCGCGCAGTCCATAGGCGCACGCGATATGGCTGCCGCACGGCGTATCGGCTGGCGTGGCATTCGCTTG
GGCGCCGGACTCGCGCTACTGACCGGCACGCTGCTCTGGCTGCTACGCGAGCCGCTGCTGCATGCCTATGTCAGCGACCC
GGCCGTGATTGCCAAAGCATTGCCGCTGATGCTGTTTGTCGCGTTCTATCAAGCCTTTGATGCTGTACAGGTCATGAGCG
CATTCATCCTGCGCGCGTACAAGATCGCGCTGATCCCGACGCTGATCTACGCCGGCTCACTCTGGGGCGTGGGGCTCGGT
GGCGGCTATGTGATGGGTTTCGGCCTGATCGATGCGTTGCCCGCCTTCACGCGCGGCGCATCGGGCTTCTGGTTGGCCAA
CAGCATCAGCCTGGCAATTGCCGGAACGCTGCTGGTTCGATACTTCATGCGCGTGAGTCGCGTTGGATAA

Upstream 100 bases:

>100_bases
GCGCCGGCTGCCGCGTCAGCCCTGAGCATCCGCCGCCCGGTAGCGTCCGGACGGCGTTGCCACCCGCTGGCACGCAGCCG
GCCCCTACCCTAGAAGAACG

Downstream 100 bases:

>100_bases
CCTGAATCAATGGCGCACAGAAAAAATGCCCGCGTATGCGGGCATTTCTAATTTGGCGAAGCGGACGGGACTCGGCTACA
TCCCACAGGCTGCGGATTCG

Product: Na+ driven multidrug efflux pump (MATE family)

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVSLMGVLQALAPVAGQLYGANR
SAEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPPELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVT
VLQIAGLALKIPLNAWFIEGGYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRL
GLPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQSIGARDMAAARRIGWRGIRL
GAGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFVAFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLG
GGYVMGFGLIDALPAFTRGASGFWLANSISLAIAGTLLVRYFMRVSRVG

Sequences:

>Translated_449_residues
MSSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVSLMGVLQALAPVAGQLYGANR
SAEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPPELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVT
VLQIAGLALKIPLNAWFIEGGYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRL
GLPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQSIGARDMAAARRIGWRGIRL
GAGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFVAFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLG
GGYVMGFGLIDALPAFTRGASGFWLANSISLAIAGTLLVRYFMRVSRVG
>Mature_448_residues
SSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVSLMGVLQALAPVAGQLYGANRS
AEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPPELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVTV
LQIAGLALKIPLNAWFIEGGYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRLG
LPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQSIGARDMAAARRIGWRGIRLG
AGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFVAFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLGG
GYVMGFGLIDALPAFTRGASGFWLANSISLAIAGTLLVRYFMRVSRVG

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=375, Percent_Identity=22.6666666666667, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI38261960, Length=407, Percent_Identity=21.1302211302211, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI22907060, Length=434, Percent_Identity=23.0414746543779, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994890, Length=449, Percent_Identity=31.8485523385301, Blast_Score=197, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321821, Length=354, Percent_Identity=23.728813559322, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=364, Percent_Identity=24.7252747252747, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47891; Mature: 47760

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHH
LMGVLQALAPVAGQLYGANRSAEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVTVLQIAGLALKIPLNAWFIEG
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCEEEEEEC
GYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHH
GLPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGARDMAAARRIGWRGIRLGAGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFV
HCCCHHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLGGGYVMGFGLIDALPAFTRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SGFWLANSISLAIAGTLLVRYFMRVSRVG
CCEEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHH
LMGVLQALAPVAGQLYGANRSAEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVTVLQIAGLALKIPLNAWFIEG
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCEEEEEEC
GYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHH
GLPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGARDMAAARRIGWRGIRLGAGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFV
HCCCHHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLGGGYVMGFGLIDALPAFTRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SGFWLANSISLAIAGTLLVRYFMRVSRVG
CCEEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA