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The map label for this gene is pcm [H]

Identifier: 94311055

GI number: 94311055

Start: 2298818

End: 2299726

Strand: Reverse

Name: pcm [H]

Synonym: Rmet_2117

Alternate gene names: 94311055

Gene position: 2299726-2298818 (Counterclockwise)

Preceding gene: 94311056

Following gene: 94311054

Centisome position: 58.55

GC content: 67.77

Gene sequence:

>909_bases
ATGAGTTCCACGCCGCGCCGATCCCGCTTTCCACTTCCGCTCGATGCGGTGGTTGAGCGCAAGCCCGCGCCAGCACGAAC
CGCCGGAATGCCTGCGGTCGGCAAGCGCGCGGCATCCCCGGCGCCGGCATCGACGGCCGTCAAGCCGCGCAAGCCGGCGG
CTCCACCGGTTGCACCGCCGCCGCCGGGCAGTGCCGTGGAAGCTCGGGCGTCCGCAGCGGTGGCTGGCGGGGGTGGGATG
GCATCGGATCGGGCTCGGGCGGCATTGGCAGCCCGCCTGCGCGCCGGCGGCATTCGCGACGAGCGGGTGCTTGGCGCCAT
TGCCACGGTGCCGCGCCACCTGTTCATGGAGCCGGGCCTGGCTTCCCAGGCGTACGAAGACGCCGCGTTGCCGATTGGAC
ATCAGCAAACCATTTCGAAGCCGTCCGTGGTGGCGCGCATGATTGAGTTGCTGCGCGAAGGGATGCCATCCGATCACGCG
CTGGCGCGCGTGCTGGAGATCGGCACCGGCTGCGGTTATCAGGCGGCCGTGCTGAGTCTGGTGGCGCAGGAAGTGTTCTC
CATAGAGCGGATTCGTCCGCTGCACGAACAAGCCAAGGCGAACCTGCGGCCGTTACGCGTGCCAAACATCCGGTTGCATT
ACGGCGACGGGATGCTTGGCCTGCCGCAGGCCGCGCCGTTTTCGTCGATCATCCTGGCCGCCGCTGGCATGGAGGTTCCG
CAGGCGTTGCTGGAACAACTGGCCATCGGCGGGCGGCTGATCGCCCCGGTGGTCGCGCCCAGCTCTGGCGCGCCCGGGCA
GACCGTGACGCAGCAGTTGCTGCTGATCGAGCGCCTCAACCGGCATCGTTTTCACAGAACTGCGCTTGAAGCGGTTTTCT
TTGTGCCCTTAAAATCGGGCACCATCTGA

Upstream 100 bases:

>100_bases
TGGCTGGGGTGAGCGTGTCATCGCCACGCTGCCTTCAGTCGTTCACACCGAATCCCCGCGCCTGCCTGCGCGTATCCGTC
AGATTGCCGTACCAGTTCAA

Downstream 100 bases:

>100_bases
GTCGAACTATGCACAACATCGTGAAATCGGATTCTTTCGCGCGAGTCGGACAACGTTTAAGCGTCGTGGCGATCGCGGCC
GTCCTGGCCGCCTGCGCCAA

Product: protein-L-isoaspartate carboxylmethyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPPPPGSAVEARASAAVAGGGGM
ASDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHA
LARVLEIGTGCGYQAAVLSLVAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVP
QALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI

Sequences:

>Translated_302_residues
MSSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPPPPGSAVEARASAAVAGGGGM
ASDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHA
LARVLEIGTGCGYQAAVLSLVAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVP
QALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI
>Mature_301_residues
SSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPPPPGSAVEARASAAVAGGGGMA
SDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHAL
ARVLEIGTGCGYQAAVLSLVAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVPQ
ALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=238, Percent_Identity=31.0924369747899, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789100, Length=225, Percent_Identity=44.4444444444444, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=183, Percent_Identity=31.1475409836066, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193207222, Length=183, Percent_Identity=30.0546448087432, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17981723, Length=188, Percent_Identity=34.0425531914894, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 31613; Mature: 31482

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPP
CCCCCCCCCCCCCHHHHHCCCCCCCHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PPGSAVEARASAAVAGGGGMASDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGL
CCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
ASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHALARVLEIGTGCGYQAAVLSL
CHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
VAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVP
HHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
QALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSG
HHHHHHHHHCCEEEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
TI
CC
>Mature Secondary Structure 
SSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPP
CCCCCCCCCCCCHHHHHCCCCCCCHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PPGSAVEARASAAVAGGGGMASDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGL
CCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
ASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHALARVLEIGTGCGYQAAVLSL
CHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
VAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVP
HHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
QALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSG
HHHHHHHHHCCEEEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
TI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA