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The map label for this gene is yfgB [H]

Identifier: 94311047

GI number: 94311047

Start: 2290832

End: 2291986

Strand: Reverse

Name: yfgB [H]

Synonym: Rmet_2109

Alternate gene names: 94311047

Gene position: 2291986-2290832 (Counterclockwise)

Preceding gene: 94311048

Following gene: 94311046

Centisome position: 58.35

GC content: 63.2

Gene sequence:

>1155_bases
ATGAATAATCTCGTCAACCTGCTCGATCTCGACGCGGACGCGCTCACTGCCTATTGCGGCGAGCTCGGCGAGAAGCCATT
CCGTGCGCGGCAGTTGCAGCGCTGGATCCACCAGTTTGGAGCCAGCCACTTCGACGCCATGACGGATCTCGCCAAATCGC
TGCGCGAAAAGCTCGCGACGCGTGCCGAGATTCGCTCGCCCGCTGCCATTTCGGACCATACGTCGTCCGATGGCACGCGC
AAATGGCTGCTCGATGTGGGGGCCGGCAACGCGGTGGAGACGGTGTATATCCCCGAGGACACGCGCGGCACGCTGTGTGT
GTCGTCGCAGGCGGGGTGCGCCGTCAATTGCCGATTCTGTTCGACCGGCAAGCAAGGTTTTTCGCGCAATCTCTCCACCG
GCGAGATTATCGGCCAGCTCTGGATGGCCGAGTTCGCGATGCGTGCCCAGCTGGGTCGCGGCCCCAAGGACGAGCGTGTC
ATCTCCAATGTGGTGATGATGGGCATGGGCGAGCCGCTGCTCAACTATGACGCGGTCGTGCCGGCCATGCGGCTGATGCT
CGATGATAATGCGTACGGCCTGTCGCGTCGGCGCGTGACGTTGTCCACGTCCGGCGTAGTGCCGATGATGGACCGTCTGT
CGAAGGACTTGCCCGTGGCGCTGGCGGTTTCGCTGCACGCCTCGAACGATGCGCTGCGCGACGTGCTCGTGCCGCTCAAC
CGCAAGTACCCGCTGGCGGAGCTGATGGCGGCTTGTCGCCGCTACCTGGAATTCGCTCCGCGCGACTTCATTACTTTCGA
ATACTGCATGCTCGATGGCGTCAACGATGGCGTCGAACATGCGCGTGAACTGCTGAAGCTTGTGGCCGACGTGCCTTGCA
AGTTCAACCTGATTCCGTTCAACCCGTTCCCGGAATCGGGGCTGAAGCGCTCGAACAACGAGCAGATTCGCCGCTTCGCC
CAGGTACTGATGGATGCGGGGATTGTCACGACGATCCGCAAAACACGTGGCGACGACATCGACGCCGCGTGTGGCCAGCT
TGCTGGCGAAGTCATGGATCGTACCCGTCTGGCCGAGCGCGGCAAGTTCGGCAAGATCACGCCACTGGTGCCGGTGGCTG
ACGCCGGCGCCCCCCGGGAGGCCCGTCCTGCATGA

Upstream 100 bases:

>100_bases
GCCGTGGAAGTGGCTTTCTTCTTCCCGGGCATGAACGTTTACAGCCGCTGATTGTCTCTGACATCACGACTGAACCTGTA
GTCAAGTTGGAACTGCCGTC

Downstream 100 bases:

>100_bases
CCCGTCTGATCGTTGCTGCCCTGTTCGGGCTGATGCTGTCCGCCTGTTCGTTGCCGCCTGCACCCGCCCAGGACATCAAG
ACCGCTTCCGATCAGACTCC

Product: Radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLATRAEIRSPAAISDHTSSDGTR
KWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
RKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAERGKFGKITPLVPVADAGAPREARPA

Sequences:

>Translated_384_residues
MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLATRAEIRSPAAISDHTSSDGTR
KWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
RKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAERGKFGKITPLVPVADAGAPREARPA
>Mature_384_residues
MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLATRAEIRSPAAISDHTSSDGTR
KWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
RKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAERGKFGKITPLVPVADAGAPREARPA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=361, Percent_Identity=56.786703601108, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42266; Mature: 42266

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLAT
CCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAEIRSPAAISDHTSSDGTRKWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFC
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERVISNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCC
RKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKITPLVPVADAGAPREARPA
CCCCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLAT
CCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAEIRSPAAISDHTSSDGTRKWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFC
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERVISNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCC
RKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKITPLVPVADAGAPREARPA
CCCCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA