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The map label for this gene is rlmB [H]

Identifier: 94311029

GI number: 94311029

Start: 2269958

End: 2270701

Strand: Reverse

Name: rlmB [H]

Synonym: Rmet_2091

Alternate gene names: 94311029

Gene position: 2270701-2269958 (Counterclockwise)

Preceding gene: 94311030

Following gene: 94311028

Centisome position: 57.81

GC content: 66.53

Gene sequence:

>744_bases
ATGGCCAAACACAAACTCCTGATCGGCTTTCATGCCGTCAACGCACGCCTGCGCCAGAACGCGCAGAGCGTGTCCGATAT
CTACATCGAAGCGAATCGCCGTGACCGGCGCATGCAGGACTTTATCCGCCTGGCGGAGAGTCTTGGCGTGACGCTGCATC
CGGTTGATGCCGAGCGCCTGCGCGGCATGGCCGGCACGGATCGCCACCAGGGCGTGGTGGCGCGGGCCGATGATGTCTCG
CTTGCGCTCAACCTCGACGAACTCCTGGATGGCATCGAAGGCACGCCGCTATTGCTCCTGCTCGACGGCGTCACCGATCC
GCACAACCTCGGCGCATGCCTGCGCGTGGCCGATGGCGCTGGCGCTCACGCGGTGATCGCGCCGAAGGACCGTAGCGTGG
GTCTGAACGCCACGGTGGCGAAGGTTGCCAGCGGCGCGGCCGAAACCGTGCCGTACATCACGGTGACGAACCTTGCGCGC
ACGCTGCGCGAACTGCAGGAGCGTGGCATCTGGGTGATCGGCACGGCTGACGGCACCGAGAAGGCGCTGTACGACGTCGA
CCTGAAGGGACCGACCGCCATCGTCATGGGCGCGGAAGGCGAGGGTATGCGCCGCCTGACGCGCGAGACTTGCGATGAAC
TGGTGGCAATTCCGATGGCGGGGGGCGTCGAGAGCCTGAACGTGTCGGTGGCCAGCGGCGTGTGCCTGTACGAAGCGGTG
CGTCAGCGCCGTCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
ACCAAGCGCGCGCCCAAGCCCGCCGGCAAGAGCACGCGGCCGGCCCGCAAGCGCTAATCTGGCGCGTTTGAAGTTGGCCA
TGGCGGGTACAATGCGCGCC

Downstream 100 bases:

>100_bases
TCATGGATAGCCTCGCCCTTGAGCAGGCGCGTGGCTGGGTTGCCGAGGCCCGTCAGATCTTTGTGCTGACGGGTGCCGGG
ATTTCCGCGGAGTCCGGTGT

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MAKHKLLIGFHAVNARLRQNAQSVSDIYIEANRRDRRMQDFIRLAESLGVTLHPVDAERLRGMAGTDRHQGVVARADDVS
LALNLDELLDGIEGTPLLLLLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNATVAKVASGAAETVPYITVTNLAR
TLRELQERGIWVIGTADGTEKALYDVDLKGPTAIVMGAEGEGMRRLTRETCDELVAIPMAGGVESLNVSVASGVCLYEAV
RQRRLPR

Sequences:

>Translated_247_residues
MAKHKLLIGFHAVNARLRQNAQSVSDIYIEANRRDRRMQDFIRLAESLGVTLHPVDAERLRGMAGTDRHQGVVARADDVS
LALNLDELLDGIEGTPLLLLLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNATVAKVASGAAETVPYITVTNLAR
TLRELQERGIWVIGTADGTEKALYDVDLKGPTAIVMGAEGEGMRRLTRETCDELVAIPMAGGVESLNVSVASGVCLYEAV
RQRRLPR
>Mature_246_residues
AKHKLLIGFHAVNARLRQNAQSVSDIYIEANRRDRRMQDFIRLAESLGVTLHPVDAERLRGMAGTDRHQGVVARADDVSL
ALNLDELLDGIEGTPLLLLLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNATVAKVASGAAETVPYITVTNLART
LRELQERGIWVIGTADGTEKALYDVDLKGPTAIVMGAEGEGMRRLTRETCDELVAIPMAGGVESLNVSVASGVCLYEAVR
QRRLPR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=266, Percent_Identity=28.1954887218045, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=53.9419087136929, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1790083, Length=151, Percent_Identity=34.4370860927152, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788935, Length=208, Percent_Identity=28.3653846153846, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=25, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=250, Percent_Identity=29.2, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26518; Mature: 26387

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHKLLIGFHAVNARLRQNAQSVSDIYIEANRRDRRMQDFIRLAESLGVTLHPVDAERL
CCCCEEEEEEHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCHHHH
RGMAGTDRHQGVVARADDVSLALNLDELLDGIEGTPLLLLLDGVTDPHNLGACLRVADGA
CCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEECCC
GAHAVIAPKDRSVGLNATVAKVASGAAETVPYITVTNLARTLRELQERGIWVIGTADGTE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
KALYDVDLKGPTAIVMGAEGEGMRRLTRETCDELVAIPMAGGVESLNVSVASGVCLYEAV
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCEEEHHHCCHHHHHHH
RQRRLPR
HHHCCCC
>Mature Secondary Structure 
AKHKLLIGFHAVNARLRQNAQSVSDIYIEANRRDRRMQDFIRLAESLGVTLHPVDAERL
CCCEEEEEEHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCHHHH
RGMAGTDRHQGVVARADDVSLALNLDELLDGIEGTPLLLLLDGVTDPHNLGACLRVADGA
CCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEECCC
GAHAVIAPKDRSVGLNATVAKVASGAAETVPYITVTNLARTLRELQERGIWVIGTADGTE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
KALYDVDLKGPTAIVMGAEGEGMRRLTRETCDELVAIPMAGGVESLNVSVASGVCLYEAV
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCEEEHHHCCHHHHHHH
RQRRLPR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]