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The map label for this gene is npdA [H]

Identifier: 94311028

GI number: 94311028

Start: 2269221

End: 2269955

Strand: Reverse

Name: npdA [H]

Synonym: Rmet_2090

Alternate gene names: 94311028

Gene position: 2269955-2269221 (Counterclockwise)

Preceding gene: 94311029

Following gene: 94311026

Centisome position: 57.79

GC content: 66.53

Gene sequence:

>735_bases
ATGGATAGCCTCGCCCTTGAGCAGGCGCGTGGCTGGGTTGCCGAGGCCCGTCAGATCTTTGTGCTGACGGGTGCCGGGAT
TTCCGCGGAGTCCGGTGTGCCGACGTTCCGCGATGCGCTGACCGGCCTATGGGAGCGCTTCGATCCCGAGGAACTGGCGA
CGGAAGAAGCGTACCGGCGTCAACCTGCGCTGGTGTGGCAGTGGTATCAGCACCGGCGCGAGCTGGTGGCGGCGGCCCGG
CCGAATCCAGCCCACCATGCGCTTGCGGTGCTGGCCCGGCTGAAGTCGATGACGCTAGTGACGCAGAATATCGATGGCCT
GCATCAGAAGGCGGGTAGCGAGCAGGTGGTGGAGCTGCACGGCAACCTCTTCGCCAACAAATGGCTTGATGGCTGTGGTC
GTTGCGATACCGTGCCGCCGATTCCGGGCGATCCCCCGCGATGCGCGATATGCGGTGCGATGATGCGTCCTGGCGTGGTG
TGGTTCGGCGAGGACCTGCCGCGAGTGGCCCGTTTCCGCGCCGACCATGCGGCCGAGTCCTGTGACCTCTGCCTGGTTGT
GGGCACGTCGGGGCTGGTGTATCCGGCGGCGGCGCTGCCGGGTGTGGCGAAGGAAAACGGCGCGCGTGTGATTGTGGTGA
ATCCGAAGCCGTCAGCACTGGACGAGACGGCGGATATCGTCCTGCCCGCGCCGGCGGGTGAATGCCTGCCGTTGCTCTGG
CCGCAGGCAGTCTGA

Upstream 100 bases:

>100_bases
CAATTCCGATGGCGGGGGGCGTCGAGAGCCTGAACGTGTCGGTGGCCAGCGGCGTGTGCCTGTACGAAGCGGTGCGTCAG
CGCCGTCTGCCGCGCTGATC

Downstream 100 bases:

>100_bases
ACGACTGTTCGACTGCGGATCCGCGGCTCCCTCCAAGGTGTTGGATAGAGCCGCGATCCCGACTCAACTGGCCAGTTCGC
CGACGAGTTTTTCCAATTTC

Product: NAD-dependent protein deacetylase, SIR2 family

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRRQPALVWQWYQHRRELVAAAR
PNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELHGNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVV
WFGEDLPRVARFRADHAAESCDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLW
PQAV

Sequences:

>Translated_244_residues
MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRRQPALVWQWYQHRRELVAAAR
PNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELHGNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVV
WFGEDLPRVARFRADHAAESCDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLW
PQAV
>Mature_244_residues
MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRRQPALVWQWYQHRRELVAAAR
PNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELHGNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVV
WFGEDLPRVARFRADHAAESCDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLW
PQAV

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=249, Percent_Identity=37.7510040160643, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI6912664, Length=267, Percent_Identity=36.7041198501873, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI13787215, Length=234, Percent_Identity=40.1709401709402, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI6912662, Length=279, Percent_Identity=31.1827956989247, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI300797597, Length=228, Percent_Identity=31.140350877193, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI6912660, Length=215, Percent_Identity=32.093023255814, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI13775602, Length=242, Percent_Identity=30.9917355371901, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI63054862, Length=210, Percent_Identity=32.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI300797577, Length=245, Percent_Identity=29.3877551020408, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI13775600, Length=242, Percent_Identity=30.9917355371901, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI300797705, Length=211, Percent_Identity=31.2796208530806, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI7657575, Length=215, Percent_Identity=30.2325581395349, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=40.3508771929825, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17567771, Length=275, Percent_Identity=27.6363636363636, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990482, Length=276, Percent_Identity=26.4492753623188, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=278, Percent_Identity=26.2589928057554, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17541892, Length=230, Percent_Identity=28.695652173913, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=227, Percent_Identity=29.5154185022026, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571445, Length=263, Percent_Identity=31.5589353612167, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648389, Length=197, Percent_Identity=29.9492385786802, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17137536, Length=227, Percent_Identity=27.7533039647577, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26380; Mature: 26380

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRR
CCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
QPALVWQWYQHRRELVAAARPNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELH
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVVWFGEDLPRVARFRADHAAES
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCC
CDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLW
CCEEEEECCCCCEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCC
PQAV
CCCC
>Mature Secondary Structure
MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRR
CCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
QPALVWQWYQHRRELVAAARPNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELH
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVVWFGEDLPRVARFRADHAAES
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCC
CDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLW
CCEEEEECCCCCEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCC
PQAV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]